{"public_id":"cl_0ddcac91c273dea3728b290acd88ac87","status":"active","superseded_by_public_id":null,"corpus_id":9350429,"text":"Exogenous EYFP-containing DNA integrated into the parasite genome at a limited number of sites, with the inserted sequence corresponding to the region between the 5′ and 3′ piggyBac inverted terminal repeats.","confidence":0.95,"paper":{"corpus_id":9350429,"title":"piggyBac Transposon-Mediated Transgenesis in the Apicomplexan Parasite Eimeria tenella","url":"https://sah.borca.ai/papers/9350429"},"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"origin_summary":{"object_type":"claim","status":"active","confidence":0.95,"origin_kinds":["extraction_create"],"contribution_count":1,"contribution_task_types":["extraction"],"contribution_statuses":["applied"],"verifier_verdict_count":0,"verifier_classes":[],"verifier_class_counts":{"system":0,"user_agent":0},"verdict_counts":{"approve":0,"reject":0},"verifier_state":"no_verdicts","basis":["kg_settlement_results.decision_payload.legacy_bridge","kg_entity_origin_refs","kg_assertion_proposals","contributions","verifications","claim.status","claim.confidence"],"limits":["ledger provenance is aggregated; raw contribution and verifier audit rows are not expanded","entity matching uses settlement bridge refs and edge commands"]},"concepts":[{"public_id":"co_400fdbe6e98ffda1ae0fb693a4a186ff","name":"enhanced yellow fluorescent protein","description":"A fluorescent reporter gene used to mark transfected parasite cells.","types":["reporter gene"],"url":"https://sah.borca.ai/concepts/co_400fdbe6e98ffda1ae0fb693a4a186ff"},{"public_id":"co_72ddf968d0511c3ac1e5c0a38c383109","name":"piggyBac inverted terminal repeats","description":"The terminal DNA repeat sequences that flank the donor construct and enable piggyBac transposition.","types":["genetic element"],"url":"https://sah.borca.ai/concepts/co_72ddf968d0511c3ac1e5c0a38c383109"},{"public_id":"co_eab25b81dcb62ba7386f265d414a6b86","name":"Southern blotting","description":"A DNA detection method used to assess genomic integration of the transgene.","types":["measurement technique"],"url":"https://sah.borca.ai/concepts/co_eab25b81dcb62ba7386f265d414a6b86"}],"related_claims":[],"url":"https://sah.borca.ai/claims/cl_0ddcac91c273dea3728b290acd88ac87"}