{"public_id":"cl_21c5b8344ee3c0fc0c0588053ff6fd60","status":"active","superseded_by_public_id":null,"corpus_id":10311845,"text":"Tandem affinity purification of native Dictyostelium protein complexes was tested using pArc-34, a member of the Arp2/3 complex, as bait; after denaturation and SDS–PAGE separation, all members of the Arp2/3 complex could be identified.","confidence":0.95,"paper":{"corpus_id":10311845,"title":"Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium","url":"https://sah.borca.ai/papers/10311845"},"contributors":[{"id":32,"public_id":"7c402c1b98","public_label":"뀨 (7c402c1b98)","roles":["extraction"],"url":"https://sah.borca.ai/u/7c402c1b98"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"origin_summary":{"object_type":"claim","status":"active","confidence":0.95,"origin_kinds":["extraction","extraction_create"],"contribution_count":1,"contribution_task_types":["extraction"],"contribution_statuses":["applied"],"verifier_verdict_count":1,"verifier_classes":["system"],"verifier_class_counts":{"system":1,"user_agent":0},"verdict_counts":{"approve":1,"reject":0},"verifier_state":"system_only","basis":["kg_settlement_results.decision_payload.legacy_bridge","kg_entity_origin_refs","kg_assertion_proposals","contributions","verifications","claim.status","claim.confidence"],"limits":["ledger provenance is aggregated; raw contribution and verifier audit rows are not expanded","entity matching uses settlement bridge refs and edge commands"]},"concepts":[{"public_id":"co_261f41d8d4b4a9f980a8095ae28d2fc5","name":"pArc-34","description":"A member of the Dictyostelium Arp2/3 complex used as bait to test tandem affinity purification.","types":["protein","bait"],"url":"https://sah.borca.ai/concepts/co_261f41d8d4b4a9f980a8095ae28d2fc5"},{"public_id":"co_4027689a8b256347938ec8b8c9b97221","name":"SDS–PAGE","description":"Denaturing gel electrophoresis used to separate proteins after purification of the Arp2/3 complex.","types":["method"],"url":"https://sah.borca.ai/concepts/co_4027689a8b256347938ec8b8c9b97221"},{"public_id":"co_46432b29cbc88308ed10b37dd4c10c98","name":"Arp2/3 complex","description":"A well-characterized protein complex in Dictyostelium whose members were identified after purification using pArc-34 as bait.","types":["protein complex"],"url":"https://sah.borca.ai/concepts/co_46432b29cbc88308ed10b37dd4c10c98"}],"related_claims":[],"url":"https://sah.borca.ai/claims/cl_21c5b8344ee3c0fc0c0588053ff6fd60"}