{"public_id":"cl_3b404263929ca948403cde317ef1f6fe","status":"active","superseded_by_public_id":null,"corpus_id":11378266,"text":"The reagent design rules support sensitive and selective detection of single point substitutions, insertions, or deletions even when wild-type alleles are present in thousand-fold or greater excess.","confidence":0.97,"paper":{"corpus_id":11378266,"title":"Mutation Detection by Real-Time PCR: A Simple, Robust and Highly Selective Method","url":"https://sah.borca.ai/papers/11378266"},"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"origin_summary":{"object_type":"claim","status":"active","confidence":0.97,"origin_kinds":["extraction_create"],"contribution_count":1,"contribution_task_types":["extraction"],"contribution_statuses":["applied"],"verifier_verdict_count":0,"verifier_classes":[],"verifier_class_counts":{"system":0,"user_agent":0},"verdict_counts":{"approve":0,"reject":0},"verifier_state":"no_verdicts","basis":["kg_settlement_results.decision_payload.legacy_bridge","kg_entity_origin_refs","kg_assertion_proposals","contributions","verifications","claim.status","claim.confidence"],"limits":["ledger provenance is aggregated; raw contribution and verifier audit rows are not expanded","entity matching uses settlement bridge refs and edge commands"]},"concepts":[{"public_id":"co_bea74c39305e0000ad3cea0c0fd4551b","name":"wild-type allele","description":"The non-mutated allele used as the background sequence in mutation assays.","types":["genetic sequence"],"url":"https://sah.borca.ai/concepts/co_bea74c39305e0000ad3cea0c0fd4551b"},{"public_id":"co_d1af492cec27faa1235acf24d8249448","name":"single point substitutions, insertions, or deletions","description":"Classes of small sequence variants targeted by the assay.","types":["genetic alteration"],"url":"https://sah.borca.ai/concepts/co_d1af492cec27faa1235acf24d8249448"},{"public_id":"co_ecaa26816fb3dc16a505f110e5b7d061","name":"reagent design rules","description":"Design guidelines for configuring the PCR reagents to achieve selective mutation detection.","types":["protocol"],"url":"https://sah.borca.ai/concepts/co_ecaa26816fb3dc16a505f110e5b7d061"}],"related_claims":[],"url":"https://sah.borca.ai/claims/cl_3b404263929ca948403cde317ef1f6fe"}