{"public_id":"cl_57bd8245ceb030057cf10d5c062687c3","status":"active","superseded_by_public_id":null,"corpus_id":22131720,"text":"The MspI modification gene from Moraxella was cloned in Escherichia coli using plasmid vector pBR322.","confidence":0.99,"paper":{"corpus_id":22131720,"title":"Cloning of the MspI modification enzyme. The site of modification and its effects on cleavage by MspI and HpaII.","url":"https://sah.borca.ai/papers/22131720"},"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"origin_summary":{"object_type":"claim","status":"active","confidence":0.99,"origin_kinds":["extraction_create"],"contribution_count":1,"contribution_task_types":["extraction"],"contribution_statuses":["applied"],"verifier_verdict_count":0,"verifier_classes":[],"verifier_class_counts":{"system":0,"user_agent":0},"verdict_counts":{"approve":0,"reject":0},"verifier_state":"no_verdicts","basis":["kg_settlement_results.decision_payload.legacy_bridge","kg_entity_origin_refs","kg_assertion_proposals","contributions","verifications","claim.status","claim.confidence"],"limits":["ledger provenance is aggregated; raw contribution and verifier audit rows are not expanded","entity matching uses settlement bridge refs and edge commands"]},"concepts":[{"public_id":"co_16f96e64a7c3e597e3392bd993163736","name":"Escherichia coli","description":"The bacterial host used for cloning the MspI modification gene.","types":["host organism"],"url":"https://sah.borca.ai/concepts/co_16f96e64a7c3e597e3392bd993163736"},{"public_id":"co_beeec9e21c856c7f871ec53ed560d2eb","name":"MspI modification enzyme","description":"A DNA methylation enzyme from Moraxella associated with the MspI restriction-modification system.","types":["enzyme"],"url":"https://sah.borca.ai/concepts/co_beeec9e21c856c7f871ec53ed560d2eb"},{"public_id":"co_dc67f8af345133b235462cd85e924adc","name":"pBR322","description":"A plasmid vector used to clone the MspI modification gene.","types":["plasmid vector"],"url":"https://sah.borca.ai/concepts/co_dc67f8af345133b235462cd85e924adc"}],"related_claims":[],"url":"https://sah.borca.ai/claims/cl_57bd8245ceb030057cf10d5c062687c3"}