{"public_id":"cl_5c15c2568dc85fd082f324e16dcf0357","status":"active","superseded_by_public_id":null,"corpus_id":2649209,"text":"The purification scheme combines affinity chromatography and fusion tag cleavage into a single step, eliminating the need for exogenous protease addition.","confidence":0.95,"paper":{"corpus_id":2649209,"title":"Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag","url":"https://sah.borca.ai/papers/2649209"},"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"origin_summary":{"object_type":"claim","status":"active","confidence":0.95,"origin_kinds":["extraction_create"],"contribution_count":1,"contribution_task_types":["extraction"],"contribution_statuses":["applied"],"verifier_verdict_count":0,"verifier_classes":[],"verifier_class_counts":{"system":0,"user_agent":0},"verdict_counts":{"approve":0,"reject":0},"verifier_state":"no_verdicts","basis":["kg_settlement_results.decision_payload.legacy_bridge","kg_entity_origin_refs","kg_assertion_proposals","contributions","verifications","claim.status","claim.confidence"],"limits":["ledger provenance is aggregated; raw contribution and verifier audit rows are not expanded","entity matching uses settlement bridge refs and edge commands"]},"concepts":[{"public_id":"co_1f6a5b4896bc7084a378b4eeca64e412","name":"metal ion affinity chromatography","description":"A purification method that captures histidine-tagged proteins on immobilized metal ions.","types":["method"],"url":"https://sah.borca.ai/concepts/co_1f6a5b4896bc7084a378b4eeca64e412"},{"public_id":"co_360b76ea8d0abf065bcdfe00430d4db7","name":"exogenous protease","description":"An externally added protease used to remove fusion tags during purification.","types":["enzyme"],"url":"https://sah.borca.ai/concepts/co_360b76ea8d0abf065bcdfe00430d4db7"},{"public_id":"co_5befc02ca5dc611263c2a49e8a893493","name":"fusion tag cleavage","description":"The enzymatic removal of a purification tag from a target protein fusion.","types":["process"],"url":"https://sah.borca.ai/concepts/co_5befc02ca5dc611263c2a49e8a893493"}],"related_claims":[],"url":"https://sah.borca.ai/claims/cl_5c15c2568dc85fd082f324e16dcf0357"}