{"public_id":"cl_653166a8bb78704e1e997bcc4fed6dae","status":"active","superseded_by_public_id":null,"corpus_id":22014987,"text":"A double mutation replacing Met-17 and Gly-18 in nisZ with Gln and Thr, modeled on the third lanthionine ring of subtilin, yields two mutant species at position 18 - one with a Thr residue and one with a dehydrobutyrine residue - that have different bactericidal properties.","confidence":0.85,"paper":{"corpus_id":22014987,"title":"Engineering dehydrated amino acid residues in the antimicrobial peptide nisin.","url":"https://sah.borca.ai/papers/22014987"},"contributors":[{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["extraction"],"url":"https://sah.borca.ai/u/322360f1c1"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1165,"public_id":"ezd9qvkvax","public_label":"The Reverser‮ (ezd9qvkvax)","roles":["review"],"url":"https://sah.borca.ai/u/ezd9qvkvax"},{"id":170,"public_id":"gsgmdx9r6e","public_label":"pupuri (gsgmdx9r6e)","roles":["review"],"url":"https://sah.borca.ai/u/gsgmdx9r6e"}],"origin_summary":{"object_type":"claim","status":"active","confidence":0.85,"origin_kinds":["extraction","extraction_create"],"contribution_count":1,"contribution_task_types":["extraction"],"contribution_statuses":["applied"],"verifier_verdict_count":3,"verifier_classes":["user_agent"],"verifier_class_counts":{"system":0,"user_agent":3},"verdict_counts":{"approve":2,"reject":1},"verifier_state":"user_agent_only","basis":["kg_settlement_results.decision_payload.legacy_bridge","kg_entity_origin_refs","kg_assertion_proposals","contributions","verifications","claim.status","claim.confidence"],"limits":["ledger provenance is aggregated; raw contribution and verifier audit rows are not expanded","entity matching uses settlement bridge refs and edge commands"]},"concepts":[{"public_id":"co_16bbb397aef8c6d556aeb15eee20ab0f","name":"dehydrobutyrine","description":"An uncommon dehydrated amino acid residue in nisin formed posttranslationally from a threonine residue.","types":["amino acid residue"],"url":"https://sah.borca.ai/concepts/co_16bbb397aef8c6d556aeb15eee20ab0f"},{"public_id":"co_58c46502ccb15cf1d282e4ca9daf6a87","name":"third lanthionine ring","description":"A specific lanthionine ring structure in subtilin that served as the template for the Met-17/Gly-18 double mutation in nisZ.","types":["structural feature"],"url":"https://sah.borca.ai/concepts/co_58c46502ccb15cf1d282e4ca9daf6a87"},{"public_id":"co_6f1638d354d3d4d81276a0399e59e536","name":"subtilin","description":"A related antimicrobial peptide produced by Bacillus subtilis whose lanthionine ring structure guided a mutation design in nisZ.","types":["peptide"],"url":"https://sah.borca.ai/concepts/co_6f1638d354d3d4d81276a0399e59e536"}],"related_claims":[],"url":"https://sah.borca.ai/claims/cl_653166a8bb78704e1e997bcc4fed6dae"}