Molecular and functional characterisation of two elovl4 elongases involved in the biosynthesis of very long-chain (>C24) polyunsaturated fatty acids in black seabream Acanthopagrus schlegelii.

Elongation of very long-chain fatty acid (Elovl) 4 proteins are important fatty acyl elongases that participate in the biosynthesis of long-chain (C20-24) and very long-chain (˃C24) polyunsaturated fatty acids (LC-PUFA and VLC-PUFA, respectively) in teleost fish, especially in marine species. Moreover, knowledge of Elovl4 and other elongases such as Elovl2 has contributed to an advanced understanding of the LC-PUFA biosynthetic pathway in marine fish. In the present study, elovl4a and elovl4b were cloned from black seabream Acanthopagrus schlegelii and functionally characterised using recombinant expression in yeast. The elovl4a and elovl4b cDNA sequences included open reading frames (ORF) of 969 and 918 base pairs (bp), encoding proteins of 322 and 315 amino acids (aa), respectively. The functional characterisation of A. schlegelii Elovl4 proteins showed they were able to utilise all assayed C18-22 PUFA substrates except 22:6n-3. Moreover, it was particularly noteworthy that both A. schlegelii Elovl4a and Elovl4b proteins had the ability to elongate 20:5n-3 and 22:5n-3 to 24:5n-3, which can be potentially desaturated and β-oxidised to 22:6n-3. Tissue transcript abundance analysis showed the highest expression of elovl4a and elovl4b in brain and eye, respectively, suggesting these tissues were major sites for VLC-PUFA biosynthesis in black seabream. The functions of the A. schlegelii Elovl4-like elongases, Elovl4a and Elovl4b, characterised in the present study, along with those of the Elovl5 and fatty acyl desaturase (Fads2) proteins of A. schlegelii characterised previously, provided evidence of the biosynthetic pathways of LC-PUFA and VLC-PUFA in this teleost species.

• Publication year

  2017

• Venue

  Comparative Biochemistry and Physiology Part B Comparative Biochemistry

• Publication date

  2017-10-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.cbpb.2017.06.008PMID 28668330
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Future availability of raw materials for salmon feeds and supply chain implications: the case of Scottish farmed salmon

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  2017influential reference
• Molecular and functional characterization of a fads2 orthologue in the Amazonian teleost, Arapaima gigas.

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• Long-chain polyunsaturated fatty acid biosynthesis in chordates: Insights into the evolution of Fads and Elovl gene repertoire.

  2016influential reference
• Biosynthesis of long-chain polyunsaturated fatty acids in the African catfish Clarias gariepinus: Molecular cloning and functional characterisation of fatty acyl desaturase (fads2) and elongase (elovl2) cDNAs7

  2016influential reference
• Biosynthesis of very long-chain (>C24) polyunsaturated fatty acids in juveniles of gilthead seabream (Sparus aurata)

  2016influential reference
• Impact of sustainable feeds on omega-3 long-chain fatty acid levels in farmed Atlantic salmon, 2006–2015

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• Long-chain polyunsaturated fatty acids in fish: recent advances on desaturases and elongases involved in their biosynthesis

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• Functional characterization of a delta 6-desaturase gene from the black seabream (Acanthopagrus schlegeli)

  2011influential reference
• Biosynthesis of very long-chain fatty acids (C>24) in Atlantic salmon: cloning, functional characterisation, and tissue distribution of an Elovl4 elongase.

  2011influential reference
• Biosynthesis of long-chain polyunsaturated fatty acids in marine fish: Characterization of an Elovl4-like elongase from cobia Rachycentron canadum and activation of the pathway during early life stages

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• Optimum arginine requirement of juvenile black sea bream, Sparus macrocephalus

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• Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development.

  2010influential reference
• The key roles of elongases and desaturases in mammalian fatty acid metabolism: Insights from transgenic mice.

  2010influential reference
• Quantitative L-lysine requirement of juvenile black sea bream (Sparus macrocephalus).

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• Highly Unsaturated Fatty Acid Synthesis in Atlantic Salmon: Characterization of ELOVL5- and ELOVL2-like Elongases

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• Fish oil replacement in finfish nutrition

  2009cited by this paper
• Towards Fish Lipid Nutrigenomics: Current State and Prospects for Fin-Fish Aquaculture

  2008cited by this paper
• Docosahexaenoic acid domains: the ultimate non-raft membrane domain.

  2008cited by this paper
• Role of Stargardt-3 macular dystrophy protein (ELOVL4) in the biosynthesis of very long chain fatty acids

  2008cited by this paper
• Model-based variance-stabilizing transformation for Illumina microarray data

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• Stock enhancement programme for black sea bream, Acanthopagrus schlegelii (Bleeker), in Hiroshima Bay, Japan: a review

  2008cited by this paper
• Effect of dietary supplemental l‐carnitine on growth performance, body composition and antioxidant status in juvenile black sea bream, Sparus macrocephalus

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• Dietary phosphorus requirement of juvenile black seabream, Sparus macrocephalus

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• Changes in Lipids Containing Long- and Very Long-Chain Polyunsaturated Fatty Acids in Cryptorchid Rat Testes1

  2007cited by this paper
• Very Long-chain Polyunsaturated Fatty Acids Are the Major Acyl Groups of Sphingomyelins and Ceramides in the Head of Mammalian Spermatozoa*

  2007cited by this paper
• A Stargardt disease‐3 mutation in the mouse Elovl4 gene causes retinal deficiency of C32–C36 acyl phosphatidylcholines

  2007cited by this paper
• Very long chain fatty acids in higher animals—A review

  2006cited by this paper
• Fatty acid elongases in mammals: their regulation and roles in metabolism.

  2006influential reference
• n-3 polyunsaturated fatty acids, inflammation, and inflammatory diseases.

  2006cited by this paper
• The co-existence of two growth hormone receptors in teleost fish and their differential signal transduction, tissue distribution and hormonal regulation of expression in seabream.

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• A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus.

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• Is docosahexaenoic acid, an n−3 long-chain polyunsaturated fatty acid, required for development of normal brain function? An overview of evidence from cognitive and behavioral tests in humans and animals

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• Is docosahexaenoic acid, an n-3 long-chain polyunsaturated fatty acid, required for development of normal brain function? An overview of evidence from cognitive and behavioral tests in humans and animals.

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• Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

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• Elongation of long-chain fatty acids.

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• Zebrafish cDNA Encoding Multifunctional Fatty Acid Elongase Involved in Production of Eicosapentaenoic (20:5n-3) and Docosahexaenoic (22:6n-3) Acids

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• Feeding ecology of larval and juvenile black seabream (Acanthopagrus schlegeli) and Japanese seaperch (Lateolabrax japonicus) in Tolo Harbour, Hong Kong

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• Elovl4 mRNA distribution in the developing mouse retina and phylogenetic conservation of Elovl4 genes.

  2003influential reference
• Very long chain PUFA in murine testicular triglycerides and cholesterol esters

  2003cited by this paper
• Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish

  2003influential reference
• A new mathematical model for relative quantification in real-time RT-PCR.

  2001cited by this paper
• Incorporation, distribution, and metabolism of polyunsaturated fatty acids in the pineal gland of rainbow trout (Oncorhynchus mykiss) in vitro

  2001cited by this paper
• A vertebrate fatty acid desaturase with Δ5 and Δ6 activities

  2001cited by this paper
• Human requirement for N-3 polyunsaturated fatty acids.

  2000influential reference
• Metabolism of highly unsaturated n-3 and n-6 fatty acids.

  2000influential reference
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  1997cited by this paper
• Fatty acid desaturation and chain elongation in eukaryotes

  1996cited by this paper
• Origins and functions of egg lipids : nutritional implications

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  1993cited by this paper
• Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.

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• Unique molecular species of phosphatidylcholine containing very-long-chain (C24-C38) polyenoic fatty acids in rat brain.

  1990cited by this paper
• Phospholipid species containing long and very long polyenoic fatty acids remain with rhodopsin after hexane extraction of photoreceptor membranes.

  1988cited by this paper
• The neighbor-joining method: a new method for reconstructing phylogenetic trees.

  1987influential reference
• A novel group of very long chain polyenoic fatty acids in dipolyunsaturated phosphatidylcholines from vertebrate retina.

  1987cited by this paper
• The dynamic structure of fatty acyl chains in a phospholipid bilayer measured by deuterium magnetic resonance.

  1974cited by this paper
• The enzymic chain elongation of fatty acids by rat-liver microsomes.

  1965cited by this paper

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• Evaluation of cottonseed oil as a substitute for dietary fish oil of juvenile black seabream (Acanthopagrus schlegelii): Based on growth, lipid metabolism, antioxidant capacity and pi3k/akt pathway

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• Desaturases and elongases involved in long-chain polyunsaturated fatty acid biosynthesis in aquatic animals: From genes to functions.

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• The repertoire of the elongation of very long-chain fatty acids (Elovl) protein family is conserved in tambaqui (Colossoma macropomum): Gene expression profiles offer insights into the sexual differentiation process.

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• Regulation of long-chain polyunsaturated fatty acid biosynthesis in teleost fish.

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  2020cites this paper
• Molecular and functional characterisation of a putative elovl4 gene and its expression in response to dietary fatty acid profile in Atlantic bluefin tuna (Thunnus thynnus).

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  2019cites this paper
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  2019cites this paper
• Cloning, tissue distribution, functional characterization and nutritional regulation of a fatty acyl Elovl5 elongase in chu's croaker Nibea coibor.

  2018cites this paper
• Essential fatty acid metabolism and requirements of the cleaner fish, ballan wrasse Labrus bergylta: Defining pathways of long-chain polyunsaturated fatty acid biosynthesis

  2018cites this paper
• Cloning, tissue distribution and nutritional regulation of a fatty acyl Elovl4-like elongase in mud crab, Scylla paramamosain (Estampador, 1949).

  2018cites this paper
• Investigating the long-chain polyunsaturated fatty acid biosynthesis of the African catfish Clarias gariepinus (Burchell, 1822)

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  2018cites this paper