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pH-Dependent Selective Protein Adsorption into Mesoporous Silica

Sebastian T. Moerz,P. Huber

Published 2015 in Journal of Physical Chemistry C

ABSTRACT

The adsorption of lysozyme, cytochrome c, and myoglobin, similar-sized globular proteins of approximately 1.5 nm radius, into the mesoporous silica material Santa Barbara Amorphous-15 (SBA-15) with 3.3 nm mean pore radius has been studied photometrically for aqueous solutions containing a single protein type and for binary protein mixtures. Distinct variations in the absolute and relative adsorption behavior are observed as a function of the solution’s pH value, and thus pore wall and protein charge. The proteins exhibit the strongest binding below their isoelectric points pI, which indicates the dominance of electrostatic interactions between charged amino acid residues and the −OH groups of the silica surface in the mesopore adsorption process. Moreover, we find for competitive adsorption in the restricted, tubular pore geometry that the protein type which shows the favored binding to the pore wall can entirely suppress the adsorption of the species with lower binding affinity, even though the latter wo...

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