PURIFICAÇÃO DE FRAGMENTOS Fab POR IMAC EM AGAROSE-CM-Asp-Co(II)

RESUMO – As imunoglobulinas G (IgG) e seus fragmentos Fab, F(ab)’2 e Fv apresentam aplicacoes relevantes na area medica e de diagnostico. O elevado custo de purificacao dessas biomoleculas por tecnicas de afinidade justifica a investigacao de alternativas de baixo custo para a sua purificacao. O objetivo deste trabalho e avaliar a eficacia do quelato CM-Asp-Co(II) imobilizado em agarose na purificacao dos fragmentos Fab de IgG humana, a partir de uma solucao de IgG clivada pela enzima papaina. O efeito do sistema tamponante e do cloreto de sodio foram avaliados. O tampao Hepes na ausencia de sal, permitiu obter os fragmentos Fab com pureza superior a 90%. Esse tampao possibilitou a maior recuperacao seletiva dos fragmentos, em comparacao com o tampao Hepes contendo NaCl e com os tampoes, Tris-HCl e fosfato de sodio. Os resultados evidenciaram a potencialidade desse ligante para purificacao de fragmentos Fab.

• Publication year

  2014

• Venue

  Unknown venue

• Publication date

  2014-09-26

• Fields of study

  Not labeled

• Identifiers
  DOI 10.5151/CHEMENG-COBEQ2014-1083-21161-143063
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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