Designer self-assembling hydrogel scaffolds can impact skin cell proliferation and migration

There is a need to develop economical, efficient and widely available therapeutic approaches to enhance the rate of skin wound healing. The optimal outcome of wound healing is restoration to the pre-wound quality of health. In this study we investigate the cellular response to biological stimuli using functionalized nanofibers from the self-assembling peptide, RADA16. We demonstrate that adding different functional motifs to the RADA16 base peptide can influence the rate of proliferation and migration of keratinocytes and dermal fibroblasts. Relative to unmodified RADA16; the Collagen I motif significantly promotes cell migration and reduces proliferation.

• Publication year

  2014

• Venue

  Scientific Reports

• Publication date

  2014-11-11

• Fields of study

  Medicine, Materials Science, Chemistry, Engineering

• Identifiers
  DOI 10.1038/srep06903PMID 25384420PMCID 4227029
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The International Journal of Biochemistry & Cell Biology

  2015cited by this paper
• Scratch-wound assay.

  2011cited by this paper
• Elucidating the role of matrix stiffness in 3D cell migration and remodeling.

  2011cited by this paper
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  2010cited by this paper
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  2009cited by this paper
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  2008cited by this paper
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  2007cited by this paper
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  2007cited by this paper
• Cultured epithelial autograft (CEA) in burn treatment: three decades later.

  2007cited by this paper
• Molecular Biology of the Cell 4th edition

  2007cited by this paper
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  2005cited by this paper
• Nanoprocessing of polymers: applications in medicine, sensors, catalysis, photonics

  2005cited by this paper
• Dynamic reassembly of peptide RADA16 nanofiber scaffold.

  2005cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2003cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2000cited by this paper
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  2000cited by this paper
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  1998cited by this paper
• The role of apoptosis in wound healing.

  1998cited by this paper
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  1997cited by this paper
• Wound Healing--Aiming for Perfect Skin Regeneration

  1997cited by this paper
• General principles of wound healing.

  1997cited by this paper
• Sterilization, toxicity, biocompatibility and clinical applications of polylactic acid/polyglycolic acid copolymers.

  1996cited by this paper
• State-of-the-art burn treatment.

  1996cited by this paper
• RGD and other recognition sequences for integrins.

  1996cited by this paper
• What role does the extracellular matrix serve in skin grafting and wound healing

  1994cited by this paper
• What role does the extracellular matrix serve in skin grafting and wound healing?

  1994cited by this paper
• Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines.

  1993cited by this paper
• Integrins: versatility, modulation, and signaling in cell adhesion.

  1992cited by this paper
• Type IV collagen and fibronectin enhance human keratinocyte thymidine incorporation and spreading in the absence of soluble growth factors.

  1990cited by this paper
• Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate.

  1989cited by this paper
• Laminin inhibits human keratinocyte migration

  1988cited by this paper
• Fibronectin, as well as other extracellular matrix proteins, mediate human keratinocyte adherence.

  1985cited by this paper
• Formation of cell-to-substrate contacts during fibroblast motility: an interference-reflexion study.

  1980cited by this paper
• Three Decades Later

  1974cited by this paper
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  1953cited by this paper

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