Enhanced rhamnolipid production by Pseudomonas aeruginosa overexpressing estA in a simple medium

A modified Pseudomonas aeruginosa strain capable of overexpressing the estA gene, an encoding gene for a membrane-bound esterase, was constructed and its rhamnolipid (RML) production was studied. Fermentations using wild-type (WT) and modified P. aeruginosa strains were conducted until exhaustion of glycerol in Medium Salt Production, using two different C/N ratios. At a C/N of 83.2, the modified strain produced up to 3.9 times more RMLs than the WT, yielding a maximum concentration of 14.62 g/L RML when measured by HPLC and 22 g/L by the orcinol assay. Cell-free supernatant from the modified strain reduced surface tension to 29.4 mN/m and had a CMC of 240 mg/L and CMD of 56.05. This is the first report on the construction of an estA-based recombinant strain for RML production.

• Publication year

  2017

• Venue

  PLoS ONE

• Publication date

  2017-08-24

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1371/journal.pone.0183857PMID 28837648PMCID 5570425
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Rhamnolipids in perspective: gene regulatory pathways, metabolic engineering, production and technological forecasting.

  2016cited by this paper
• Physicochemical Properties of Rhamnolipid Biosurfactant from Pseudomonas aeruginosa PA1 to Applications in Microemulsions

  2015cited by this paper
• Rhamnolipid biosurfactant from Pseudomonas aeruginosa - from discovery to application in contemporary technology

  2015cited by this paper
• Rhamnolipid biosurfactants—past, present, and future scenario of global market

  2014cited by this paper
• Molecular characterization of vulnibactin biosynthesis in Vibrio vulnificus indicates the existence of an alternative siderophore

  2013cited by this paper
• The use of raw cheese whey and olive oil mill wastewater for rhamnolipid production by recombinant Pseudomonas aeruginosa

  2013cited by this paper
• Rhamnolipid production by Pseudomonas aeruginosa engineered with the Vitreoscilla hemoglobin gene

  2012cited by this paper
• Characterization of rhamnolipids produced by wild-type and engineered Burkholderia kururiensis

  2012cited by this paper
• Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440

  2011cited by this paper
• Evaluation of rhamnolipid production capacity of Pseudomonas aeruginosa PAO1 in comparison to the rhamnolipid over-producer strains DSM 7108 and DSM 2874

  2011cited by this paper
• The structural biology of β-barrel membrane proteins: a summary of recent reports.

  2011cited by this paper
• High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface.

  2010cited by this paper
• Crystal structure of a full-length autotransporter.

  2010cited by this paper
• Extracellular enzymes affect biofilm formation of mucoid Pseudomonas aeruginosa.

  2010cited by this paper
• Pseudomonas aeruginosa PAO1 as a model for rhamnolipid production in bioreactor systems

  2010cited by this paper
• Rhamnolipids: diversity of structures, microbial origins and roles

  2010cited by this paper
• Natural surfactants used in cosmetics: glycolipids

  2009cited by this paper
• Heterologous production of Pseudomonas aeruginosa EMS1 biosurfactant in Pseudomonas putida.

  2008cited by this paper
• Single-cell high-throughput screening to identify enantioselective hydrolytic enzymes.

  2008cited by this paper
• The Autotransporter Esterase EstA of Pseudomonas aeruginosa Is Required for Rhamnolipid Production, Cell Motility, and Biofilm Formation

  2007cited by this paper
• A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation.

  2006cited by this paper
• A generic system for the Escherichia coli cell‐surface display of lipolytic enzymes

  2005cited by this paper
• Type V Protein Secretion Pathway: the Autotransporter Story

  2004cited by this paper
• Use of methylumbeliferyl-derivative substrates for lipase activity characterization

  2003cited by this paper
• Evaluation of different carbon and nitrogen sources in production of rhamnolipids by a strain of Pseudomonas aeruginosa

  2002cited by this paper
• Rhamnolipid production by Pseudomonas aeruginosa LBI growing on soapstock as the sole carbon source

  2002cited by this paper
• A Novel Lipolytic Enzyme Located in the Outer Membrane of Pseudomonas aeruginosa

  1999cited by this paper
• High production of rhamnolipids by Pseudomonas aeruginosagrowing on ethanol

  1997cited by this paper
• Isolation, characterization, and expression in Escherichia coli of the Pseudomonas aeruginosa rhlAB genes encoding a rhamnosyltransferase involved in rhamnolipid biosurfactant synthesis.

  1994cited by this paper
• Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa.

  1994cited by this paper
• Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants

  1991cited by this paper
• CONSTITUENT ANALYSES OF GLYCOSAMINOGLYCANS

  1980cited by this paper
• Chromosomal genetics of Pseudomonas.

  1979cited by this paper

• Prospects and challenges regarding biosurfactants in advancing the petroleum industry

  2026cites this paper
• Next-generation strategies for rhamnolipid biosurfactant production: Metabolic optimization and sustainable process development

  2026cites this paper
• Hyperproduction of Rhamnolipid in P. putida by Protein and Metabolic Engineering.

  2025cites this paper
• Optimizing rhamnolipid bio-surfactant production in a bioreactor using waste glycerol

  2025cites this paper
• Critical review on revamping circular economy strategies for the co-production of biosurfactants and lipase from agro-industrial wastes through resource recovery and life cycle assessment

  2025cites this paper
• Attenuated Strains of Pseudomonas aeruginosa : A Promising Cell Factory for Rhamnolipid Production

  2025cites this paper
• Pseudomonas aeruginosa Rhamnolipids Produced by Andiroba (Carapa guianensis Aubl.) (Sapindales: Meliaceae) Biomass Waste from Amazon: A Potential Weapon Against Aedes aegypti L. (Diptera: Culicidae)

  2025cites this paper
• A critical review on scale-up strategies of biosurfactant production and its applications

  2024cites this paper
• Optimized Rhamnolipid Production by a Pseudomonas marginalis C9 Strain Isolated from a Biopurification System to Enhance Pesticide Solubilization

  2024cites this paper
• Rhamnolipids: Green Surfactant for Environmental Bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs)

  2024cites this paper
• Rhamnolipids production by Pseudomonas aeruginosa RW9 using palm oil mill effluent sludge oil as a carbon source

  2024cites this paper
• Single batch co-production of Pseudomonad polyhydroxybutyrate and rhamnolipids on wasted cooking oil under salinity stress

  2023cites this paper
• Prospects of Microbial Bio‐surfactants To Endorse Prolonged Conservation in the Pharmaceutical and Agriculture Industries

  2023cites this paper
• Sustainable rhamnolipids production in the next decade - Advancing with Burkholderia thailandensis as a potent biocatalytic strain.

  2023cites this paper
• Genome-wide analysis reveals a rhamnolipid-dependent modulation of flagellar genes in Pseudomonas aeruginosa PAO1

  2022cites this paper
• Adaptation to an Amoeba Host Leads to Pseudomonas aeruginosa Isolates with Attenuated Virulence

  2022cites this paper
• Characterization of a potent biosurfactant produced from Franconibacter sp. IITDAS19 and its application in enhanced oil recovery.

  2022cites this paper
• Overview on Glycosylated Lipids Produced by Bacteria and Fungi: Rhamno-, Sophoro-, Mannosylerythritol and Cellobiose Lipids.

  2022cites this paper
• Production and Characterization of Rhamnolipids Produced by Pseudomonas aeruginosa DBM 3774: Response Surface Methodology Approach

  2022cites this paper
• Gentamicin Augments the Quorum Quenching Potential of Cinnamaldehyde In Vitro and Protects Caenorhabditis elegans From Pseudomonas aeruginosa Infection

  2022cites this paper
• Bioreactor-scale production of rhamnolipids from food waste digestate and its recirculation into anaerobic digestion for enhanced process performance: Creating closed-loop integrated biorefinery framework.

  2022cites this paper
• Genome-Wide Analysis Reveals a RhlA-Dependent Modulation of Flagellar Genes in Pseudomonas Aeruginosa PAO1

  2021cites this paper
• Best Medium for Production and the Activity of Biosurfactant from Local Strain of Pseudomonas

  2021cites this paper
• Optimization and characterization of rhamnolipid production by Pseudomonas aeruginosa NY3 using waste frying oil as the sole carbon

  2021cites this paper
• Type 3 secretion system of Pseudomonas aeruginosa.

  2021cites this paper
• The Potential Use of Biosurfactants in Cosmetics and Dermatological Products

  2021cites this paper
• Environmentally friendly rhamnolipid production for petroleum remediation.

  2020cites this paper
• Sodium salicylate interferes with quorum-sensing-regulated virulence in chronic wound isolates of Pseudomonas aeruginosa in simulated wound fluid

  2020cites this paper
• The Use of Biosurfactants in the Bioremediation of Oil Spills in Water

  2020cites this paper
• A novel approach for the production of green biosurfactant from Pseudomonas aeruginosa using renewable forest biomass.

  2020cites this paper
• Glycerol chlorination reaction mechanism

  2020cites this paper
• Light-triggered Nitric Oxide Release Photosensitizer to Combat Bacterial Biofilm Infections.

  2020cites this paper
• Rhamnolipids produced by Pseudomonas: from molecular genetics to the market

  2020cites this paper
• Rhamnolipid production by Pseudomonas aeruginosa grown on membranes of bacterial cellulose supplemented with corn bran water extract

  2020cites this paper
• Pseudomonas aeruginosa isolates co-incubated with Acanthamoeba castellanii exhibit phenotypes similar to chronic cystic fibrosis isolates

  2020cites this paper
• Production and evaluation of mono- and di-rhamnolipids produced by Pseudomonas aeruginosa VM011

  2019cites this paper
• Terrestrial Microorganisms: Cell Factories of Bioactive Molecules with Skin Protecting Applications

  2019cites this paper
• Gut bacteria of Cuora amboinensis (turtle) produce broad-spectrum antibacterial molecules

  2019cites this paper
• Optimization and characterization of a glycolipid produced by Achromobacter sp. to use in petroleum industries

  2019cites this paper
• Optimization of rhamnolipid production from bioconversion of palm oil mill effluent (POME) waste by Pseudomonas stutzeri BK-AB12 using response surface methodology

  2018cites this paper