Immunoprotective Efficacy of Six In vivo-Induced Antigens against Actinobacillus pleuropneumoniae as Potential Vaccine Candidates in Murine Model

Six in vivo-induced (IVI) antigens—RnhB, GalU, GalT, Apl_1061, Apl_1166, and HflX were selected for a vaccine trial in a mouse model. The results showed that the IgG levels in each immune group was significantly higher than that of the negative control (P < 0.001). Except rRnhB group, proliferation of splenocytes was observed in all immunized groups and a relatively higher proliferation activity was observed in rGalU and rGalT groups (P < 0.05). In the rGalT vaccinated group, the proportion of CD4+ T cells in spleen was significant higher than that of negative control (P < 0.05). Moreover, proportions of CD4+ T cells in other vaccinated groups were all up-regulated to varying degrees. Up-regulation of both Th1 (IFN-γ, IL-2) and Th2 (IL-4) cytokines were detected. A survival rate of 87.5, 62.5, and 62.5% were obtained among rGalT, rAPL_1166, and rHflX group, respectively while the remaining three groups was only 25%. Histopathological analyses of lungs indicated that surviving animals from the vaccinated groups showed relatively normal pulmonary structure alveoli. These findings confirm that IVI antigens used as vaccine candidates provide partial protection against Actinobacillus pleuropneumoniae infection in a mouse model, which could be used as potential vaccine candidates in piglets.

• Publication year

  2016

• Venue

  Frontiers in Microbiology

• Publication date

  2016-10-21

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3389/fmicb.2016.01623PMID 27818646PMCID 5073529
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Nasal immunization with mannan-decorated mucoadhesive HPMCP microspheres containing ApxIIA toxin induces protective immunity against challenge infection with Actinobacillus pleuropneumoiae in mice.

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  2015influential reference
• An O Antigen Capsule Modulates Bacterial Pathogenesis in Shigella sonnei

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  2015cited by this paper
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  2015cited by this paper
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  2014influential reference
• ACTINOBACILLUS PLEUROPNEUMONIAE SEROPREVALENCE IN FARMED WILD BOARS IN FINLAND

  2014cited by this paper
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  2013cited by this paper
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• Immunogenicity and protective efficacy of recombinant Haemophilus parasuis SH0165 putative outer membrane proteins.

  2013influential reference
• Bacterial pathogens associated with lung lesions in slaughter pigs from 125 herds.

  2012cited by this paper
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  2012cited by this paper
• Magnetic beads-based enzymatic spectrofluorometric assay for rapid and sensitive detection of antibody against ApxIVA of Actinobacillus pleuropneumoniae.

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  2012cited by this paper
• Antimicrobial resistance of Actinobacillus pleuropneumoniae isolated from swine.

  2012cited by this paper
• Antimicrobial Susceptibilities and Resistance Genes of Canadian Isolates of Actinobacillus pleuropneumoniae

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  2012cited by this paper
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  2011influential reference
• Evaluation of Actinobacillus pleuropneumoniae diagnostic tests using samples derived from experimentally infected pigs.

  2011cited by this paper
• Field experience with two different vaccination strategies aiming to control infections with Actinobacillus pleuropneumoniae in a fattening pig herd

  2010cited by this paper
• Risk factors associated with pleuritis and cranio-ventral pulmonary consolidation in slaughter-aged pigs.

  2010cited by this paper
• Pleuritis in slaughter pigs: relations between lung lesions and bacteriology in 10 herds with high pleuritis.

  2010influential reference
• Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice

  2010cited by this paper
• AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge.

  2009cited by this paper
• Immunogenicity and protective efficacy of ApxIA and ApxIIA DNA vaccine against Actinobacillus pleuropneumoniae lethal challenge in murine model.

  2009cited by this paper
• Positive role for rApxIVN in the immune protection of pigs against infection by Actinobacillus pleuropneumoniae.

  2009cited by this paper
• Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae.

  2008cited by this paper
• Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemically infected swine herd

  2008cited by this paper
• Development of a multiplex PCR test for identification of Actinobacillus pleuropneumoniae serovars 1, 7, and 12.

  2008cited by this paper
• Selective capture of transcribed sequences (SCOTS) of Actinobacillus pleuropneumoniae in the chronic stage of disease reveals an HlyX-regulated autotransporter protein.

  2007cited by this paper
• Development of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae infection.

  2006cited by this paper
• Argonaute—a database for gene regulation by mammalian microRNAs

  2005cited by this paper
• Use of In Vivo-Induced Antigen Technology for Identification of Escherichia coli O157:H7 Proteins Expressed during Human Infection

  2005cited by this paper
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  2005cited by this paper
• Identification of Group A Streptococcus Antigenic Determinants Upregulated In Vivo

  2005cited by this paper
• Biofilm formation is prevalent among field isolates of Actinobacillus pleuropneumoniae.

  2005cited by this paper
• Detection of Actinobacillus pleuropneumoniae by PCR on Field Strains from Healthy and Diseased Pigs

  2003cited by this paper
• Construction of an Actinobacillus pleuropneumoniae Serotype 2 Prototype Live Negative-Marker Vaccine

  2002cited by this paper
• Actinobacillus species and their role in animal disease.

  2000cited by this paper
• IVIAT: a novel method to identify microbial genes expressed specifically during human infections.

  2000cited by this paper
• Intramuscular immunization with genetically inactivated (ghosts) Actinobacillus pleuropneumoniae serotype 9 protects pigs against homologous aerosol challenge and prevents carrier state.

  2000cited by this paper
• Identification of Actinobacillus pleuropneumoniae virulence genes using signature-tagged mutagenesis in a swine infection model.

  2000cited by this paper
• Isolation and characterization of mini-Tn10 lipopolysaccharide mutants of Actinobacillus pleuropneumoniae serotype 1.

  1999cited by this paper
• Susceptibility testing of Actinobacillus pleuropneumoniae in Denmark. Evaluation of three different media of MIC-determinations and tablet diffusion tests.

  1999influential reference
• Pigs aerogenously immunized with genetically inactivated (ghosts) or irradiated Actinobacillus pleuropneumoniae are protected against a homologous aerosol challenge despite differing in pulmonary cellular and antibody responses.

  1999cited by this paper
• Cross-protection experiments in pigs vaccinated with Actinobacillus pleuropneumoniae subtypes 1A and 1B.

  1995cited by this paper
• Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene.

  1990cited by this paper
• Identification and serotyping of Haemophilus pleuropneumoniae by coagglutination test

  1983cited by this paper
• The identification of a Haemophilus-like organism associated with pneumonia and pleurisy in the pig.

  1961cited by this paper
• Oral immunization.

  1948cited by this paper

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