Boron- and nitrogen-doped photoluminescent polymer carbon nanoparticles as nanosensors for imaging detection of Cu2+ and biothiols in living cells

Boron and nitrogen co-doped polymer carbon nanoparticles (BNPCNPs) were synthesized by a facile hydrothermal treatment using uric acid as a nitrogen source, and phenylboronic acid as a boron source for the first time. The nanoparticles with sizes in the range of 90 to 180 nm show excellent and stable fluorescence properties. Moreover, these BNPCNPs showed highly efficient fluorescence quenching ability in the presence of copper (Cu2+) ions due to the formed nonfluorescent metal complexes via robust Cu2+–O or Cu2+–N interactions with the O and N of fluorescent BNPCNPs, which allowed the analysis of Cu2+ ions in the range of 0.0033 to 80 μM. Besides Cu2+ sensing, when biothiols were added, the quenched BNPCNPs-Cu2+ system could be regained via the effective coordination/chelation interactions between Cu2+ ions and the plentiful mercapto and amino groups of biothiols. In the light of this theory, simple biothiol sensors were fabricated without complicated, costly and time-consuming operations. The linear range and the limit of detection of the BNPCNPs-Cu2+ system were 0.0078–80 μM and 2.1 nM for Lcy, 0.0085–85 μM and 2.7 nM for Hcy, and 0.013–89 μM and 4.2 nM for GSH, respectively. Especially, the nanoprobe exhibits good cell membrane permeability and excellent biocompatibility by HeLa cells assay, which is favorable for bioimaging applications. So this BNPCNPs probe can be further used for imaging of biothiols in living cells.

• Publication year

  2017

• Venue

  RSC Advances

• Publication date

  2017-10-06

• Fields of study

  Biology, Materials Science, Chemistry

• Identifiers
  DOI 10.1039/C7RA07683E
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• The nanoprobe shows good cell membrane permeability and biocompatibility in HeLa cells, supporting imaging of biothiols in living cells.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review
• A quenched BNPCNPs-Cu2+ system can be restored by biothiols, supporting simple sensing of L-cysteine, homocysteine, and glutathione with nanomolar detection limits.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review
• The nanoparticles act as a fluorescent nanosensor whose emission is efficiently quenched by Cu2+ ions, enabling Cu2+ detection across 0.0033 to 80 μM.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review
• Boron- and nitrogen-doped polymer carbon nanoparticles were synthesized from uric acid and phenylboronic acid by a hydrothermal route.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review

• biothiols

  biomolecule, analyte

  Thiols in biological systems, including cysteine, homocysteine, and glutathione, used as the target class for sensing and imaging.

  Aliases: L-cysteine, homocysteine, glutathione

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review
• boron- and nitrogen-doped polymer carbon nanoparticles

  material, nanoparticle

  Fluorescent carbon nanoparticles co-doped with boron and nitrogen and used here as the sensing and imaging platform.

  Aliases: BNPCNPs, boron- and nitrogen-doped photoluminescent polymer carbon nanoparticles

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review
• cu2+ ions

  ion, analyte

  Divalent copper ions targeted as the analyte for fluorescence-quenching detection.

  Aliases: copper(II) ions

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review
• hela cells

  cell line, model system

  A human cervical cancer cell line used to assess biocompatibility and intracellular imaging performance.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review
• hydrothermal treatment

  method, synthesis process

  A high-temperature, sealed-vessel synthesis process used to prepare the nanoparticles.

  Aliases: hydrothermal route

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review
• phenylboronic acid

  chemical compound, precursor

  An aromatic boronic acid used as the boron source in the nanoparticle synthesis.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review
• uric acid

  chemical compound, precursor

  An organic compound used as the nitrogen source in the nanoparticle synthesis.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review뀨 (7c402c1b98) reviewAK (4715169a40) reviewAnonymous (12632b8b5f) reviewjaeyoung (rb9u4m834j) review

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