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Effective thermal pretreatment of water hyacinth (Eichhornia crassipes) for the enhancement of biomethanation: VIT® gene probe technology for microbial community analysis with special reference to methanogenic Archaea

S. Ali,Jianzhong Sun

Published 2019 in Journal of Environmental Chemical Engineering

ABSTRACT

Abstract Water Hyacinth (WH) remains an intractable challenge worldwide hindering the aquatic ecosystems but a potential lignocellulosic biomass for the generation of renewable biogas through Anaerobic Digestion (AD). Here, autoclave pretreatment at 121 °C for 30 min was utilized to pretreat WH in order to enhance its solubilization. Cow Dung (CD), as a source of microorganisms, was mixed with the pretreated WH (1:1 w/w dry basis) followed by mixing with water to form slurry and digested for 50 days to explore its prospective biomethanation. The untreated WH exhibited its highest yield of methane (113 ± 11 mL CH4/g VS) on the 35th day whereas for the pretreated WH slurry, this yield was increased to 150 ± 9 mL CH4/g VS on the 21st day. Clearly, the autoclave pretreated WH exhibited enhanced CH4-containing biogas production as the highest methane yield obtained in a short time. The microbial communities were tested by using VIT® gene probe. The total bacterial communities were quantified by qPCR targeting 16S rRNA genes using a LIVE/DEAD test. The functional gene, mcrA, was used as a target for detecting the most abundant methanogenic Archaea. The results revealed that six bacterial phyla were dominant. Of these, Proteobacteria, Bacteroidetes and Chloroflexi were the most abundant phyla (86.1 ± 5.2%). The qPCR showed viable and total cell counts of 2.45E + 09 and 5.11E + 09 cells/mL, respectively. The mcrA gene qPCR decreased by approximately 10.7% viable cells overall after the biomethanation. Using specific gene probes, the methanogenic Archaea were identified as members of the genus Methanosarcina.

PUBLICATION RECORD

  • Publication year

    2019

  • Venue

    Journal of Environmental Chemical Engineering

  • Publication date

    2019-02-01

  • Fields of study

    Biology, Chemistry, Environmental Science

  • Identifiers
    DOI 10.1016/J.JECE.2018.102853
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar

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