Expression of hepatitis C virus nonstructural 4B in transgenic mice

Hepatitis C virus (HCV) is a pathogen that is of great medical significance in chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide. Although the HCV proteins have been intensively investigated over the past decade, the biochemical functions of the NS4B protein are still largely unknown. To investigate NS4B as a potential causative agent of liver disease, transgenic mice expressing the NS4B protein in liver tissue were produced. The transgenic animals were phenotypically similar to their normal littermates for up to 18 months of age. Our results suggest that the HCV NS4B protein is not directly cytopathic or oncogenic in our transgenic mice model.

• Publication year

  2006

• Venue

  Experimental and Molecular Medicine

• Publication date

  2006-06-30

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/emm.2006.29PMID 16819282
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  1999cited by this paper
• The nineteenth United States‐Japan joint hepatitis panel meeting

  1998cited by this paper
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  1997cited by this paper
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• Hepatitis B and C virus hepatocarcinogenesis: lessons learned and future challenges.

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