High Throughput Identification and Quantification of Phospholipids in Complex Mixtures.

We present a fully automatic method, autoP, for identification and quantification of lipids in complex lipid mixtures from 1D (31)P and 2D (1)H-(31)P NMR spectra. The (31)P chemical shifts in lipids are highly sensitive to experimental conditions such as pH and temperature, so the present method uses the much more unambiguous (1)H chemical shifts for assignment and (31)P intensities for quantification. By using 2D (1)H-(31)P total correlation spectroscopy (TOCSY) correlation experiments, we demonstrate that approximately 20 different lipids can be automatically and unambiguously assigned and quantified by this automatic method.

• Publication year

  2016

• Venue

  Analytical Chemistry

• Publication date

  2016-02-02

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1021/acs.analchem.5b03798PMID 26797086
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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