Thyrotropin-releasing hormone-induced subcellular redistribution and down-regulation of G11alpha: analysis of agonist regulation of coexpressed G11alpha species variants.

Human embryonic kidney 293 cells that had been transfected to express the long isoform of the rat thyrotropin-releasing hormone (TRH) receptor (clone E2) were further transfected with a cDNA encoding the murine version of G11alpha. A clone was isolated (clone E2M11) that stably expressed murine as well as the endogenous human G11alpha. Subcellular fractionation demonstrated identical cellular distribution of the two species variants of this G protein. Sustained exposure of clone E2M11 cells to TRH resulted in substantial cellular redistribution and reduction in total cellular levels of G11alpha immunoreactivity. Fractions of both the exogenously introduced murine and endogenously expressed human isoforms of G11alpha were transferred from plasma membranes to low density membranes (detected as a shift from middle to low density regions on sucrose density gradients) and cytosol fractions. The plasma membrane redistribution to low density membrane was accompanied by a parallel redistribution of G protein beta subunits; however, there was no increase in beta subunits in the cytosol. The total cellular amount of G11alpha subunits was decreased to 21% and 59% for human and murine isoforms, respectively, and beta subunits were decreased to 68% after sustained treatment with TRH compared with controls (100%). Such data are consistent with the notion that the agonist-occupied long isoform of the rat TRH receptor may be able to partially differentiate between the endogenous (human) and exogenous (murine) G11alpha. This was not a reflection that the murine G protein was expressed but incorrectly folded as both species variants of G11alpha were solubilized equally from E2M11 membranes by sodium cholate. Using this system, we demonstrate both agonist-induced subcellular redistribution and down-regulation of G11alpha and beta subunit proteins in response to activation of a phospholipase C coupled receptor.

• Publication year

  1996

• Venue

  Molecular Pharmacology

• Publication date

  1996-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0026-895x(25)08778-4PMID 8609892
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The role of palmitoylation of the guanine nucleotide binding protein G11 alpha in defining interaction with the plasma membrane.

  1995cited by this paper
• Lipid Modifications of Trimeric G Proteins (*)

  1995cited by this paper
• Differential Interaction with and Regulation of Multiple G-proteins by the Rat A3 Adenosine Receptor (*)

  1995cited by this paper
• Degradation of G11 alpha/Gq alpha is accelerated by agonist occupancy of alpha 1A/D, alpha 1B, and alpha 1C adrenergic receptors.

  1995cited by this paper
• The dynamic role of palmitoylation in signal transduction.

  1995cited by this paper
• Gonadotrophin-releasing hormone receptor agonist-mediated down-regulation of Gq alpha/G11 alpha (pertussis toxin-insensitive) G proteins in alpha T3-1 gonadotroph cells reflects increased G protein turnover but not alterations in mRNA levels.

  1995cited by this paper
• Down-regulation of the G-proteins Gq alpha and G11 alpha by transfected human M3 muscarinic acetylcholine receptors in Chinese hamster ovary cells is independent of receptor down-regulation.

  1995cited by this paper
• Visualization of the thyrotropin-releasing hormone receptor and its ligand during endocytosis and recycling.

  1995cited by this paper
• Agonist-induced transfer of the alpha subunits of the guanine-nucleotide-binding regulatory proteins Gq and G11 and of muscarinic m1 acetylcholine receptors from plasma membranes to a light-vesicular membrane fraction.

  1994cited by this paper
• Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G alpha q and not G alpha 11.

  1994cited by this paper
• Agonist regulation of cellular Gs alpha-subunit levels in neuroblastoma x glioma hybrid NG108-15 cells transfected to express different levels of the human beta 2 adrenoceptor.

  1994cited by this paper
• Concurrent specific immunological detection of both primate and rodent forms of the guanine nucleotide binding protein G11 alpha following their coexpression.

  1994cited by this paper
• The long isoform of the rat thyrotropin-releasing hormone receptor down-regulates Gq proteins.

  1994cited by this paper
• Characterization of caveolin-rich membrane domains isolated from an endothelial-rich source: implications for human disease

  1994cited by this paper
• Regulation of basal adenylate cyclase activity in neuroblastoma x glioma hybrid, NG108-15, cells transfected to express the human beta 2 adrenoceptor: evidence for empty receptor stimulation of the adenylate cyclase cascade.

  1994cited by this paper
• Enhanced degradation of the phosphoinositidase C-linked guanine-nucleotide-binding protein Gq alpha/G11 alpha following activation of the human M1 muscarinic acetylcholine receptor expressed in CHO cells.

  1993cited by this paper
• Signal transducing molecules and glycosyl-phosphatidylinositol-linked proteins form a caveolin-rich insoluble complex in MDCK cells

  1993cited by this paper
• Agonist-stimulated internalization of the thyrotropin-releasing hormone receptor is dependent on two domains in the receptor carboxyl terminus.

  1993cited by this paper
• Beta-adrenergic receptor sequestration. A potential mechanism of receptor resensitization.

  1993cited by this paper
• The human muscarinic M1 acetylcholine receptor, when express in CHO cells, activates and downregulates both Gq alpha and G11 alpha equally and non-selectively.

  1993cited by this paper
• Agonist regulation of cellular G protein levels and distribution: mechanisms and functional implications.

  1993cited by this paper
• Plasma-membrane-independent pool of the alpha subunit of the stimulatory guanine-nucleotide-binding regulatory protein in a low-density-membrane fraction of S49 lymphoma cells.

  1992cited by this paper
• Translocation of alpha subunits of stimulatory guanine nucleotide-binding proteins through stimulation of the prostacyclin receptor in mouse mastocytoma cells.

  1992cited by this paper
• Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity

  1992cited by this paper
• G alpha 11 and G alpha q guanine nucleotide regulatory proteins differentially modulate the response to thyrotropin-releasing hormone in Xenopus oocytes.

  1992cited by this paper
• Widespread distribution of Gq alpha/G11 alpha detected immunologically by an antipeptide antiserum directed against the predicted C-terminal decapeptide.

  1991cited by this paper
• Two alpha subunits of the Gq class of G proteins stimulate phosphoinositide phospholipase C-beta 1 activity.

  1991cited by this paper
• Delta-opioid-receptor-mediated inhibition of adenylate cyclase is transduced specifically by the guanine-nucleotide-binding protein Gi2.

  1990cited by this paper
• Down-regulation of Gi sub-types by prolonged incubation of adipocytes with an A1 adenosine receptor agonist.

  1990cited by this paper
• G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.

  1990cited by this paper
• Turning off the signal: desensitization of β‐adrenergic receptor function

  1990cited by this paper
• Regulation of bombesin-stimulated inositol 1,4,5-trisphosphate generation in Swiss 3T3 fibroblasts by a guanine-nucleotide-binding protein.

  1990cited by this paper
• Prostaglandin E1-mediated, cyclic AMP-independent, down-regulation of Gs alpha in neuroblastoma x glioma hybrid cells.

  1990cited by this paper
• Isoproterenol stimulates shift of G proteins from plasma membrane to pinocytotic vesicles in rat adipocytes: a possible means of signal dissemination.

  1990cited by this paper
• Calculating receptor number from binding experiments using same compound as radioligand and competitor.

  1989cited by this paper
• Stimulation of beta-adrenergic receptors of S49 lymphoma cells redistributes the alpha subunit of the stimulatory G protein between cytosol and membranes.

  1989cited by this paper
• Different sensitivity of ATP + Mg + Na (I) and Pi + Mg (II) dependent types of ouabain binding to phospholipase A2

  1988cited by this paper
• Foetal-calf serum stimulates a pertussis-toxin-sensitive high-affinity GTPase activity in rat glioma C6 BU1 cells.

  1987cited by this paper

• Amelioration of Glutamate-induced Toxicity by a New Thyrotropin-releasing Hormone (TRH) Analogue PYR-l-(2,5-Dibromo)-His-l-ProNH2.

  2025cites this paper
• Biochemical and physiological insights into TRH receptor-mediated signaling

  2022cites this paper
• Identification of a preassembled TRH receptor-G(q/11) protein complex in HEK293 cells.

  2012influential citation
• Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells.

  2012cites this paper
• Up-regulation of adenylylcyclases I and II induced by long-term adaptation of rats to morphine fades away 20 days after morphine withdrawal.

  2011cites this paper
• Protein alterations induced by long‐term agonist treatment of HEK293 cells expressing thyrotropin‐releasing hormone receptor and G11α protein

  2010cites this paper
• Long-term adaptation to high doses of morphine causes desensitization of mu-OR- and delta-OR-stimulated G-protein response in forebrain cortex but does not decrease the amount of G-protein alpha subunits.

  2010cites this paper
• NEUROPROTECTIVE EFFECT OF THYROTROPIN-RELEASING HORMONE (TRH) AGAINST GLUTAMATE TOXICITY IN VITRO

  2009cites this paper
• Subcellular shifts of trimeric G-proteins following activation of Baker’s yeast by glucose

  2008cites this paper
• The Time-Course of Agonist-Induced Solubilization of Trimeric Gqα/G11α Proteins Resolved by Two-Dimensional Electrophoresis

  2008cites this paper
• Subcellular redistribution of trimeric G-proteins--potential mechanism of desensitization of hormone response: internalization, solubilization, down-regulation.

  2008cites this paper
• The time-course of agonist-induced solubilization of trimeric G(q)alpha/G(11)alpha proteins resolved by two-dimensional electrophoresis.

  2008cites this paper
• Prolonged agonist stimulation does not alter the protein composition of membrane domains in spite of dramatic changes induced in a specific signaling cascade

  2007cites this paper
• Role of receptor‐mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity

  2007cites this paper
• Disruption of the Plasma Membrane Integrity by Cholesterol Depletion Impairs Effectiveness of TRH Receptor-Mediated Signal Transduction via Gq/G11α Proteins

  2007cites this paper
• An analog of thyrotropin-releasing hormone (TRH) is neuroprotective against glutamate-induced toxicity in fetal rat hippocampal neurons in vitro.

  2007cites this paper
• The time-course of agonist-induced solubilisation of trimeric Gqa/G11a proteins resolved by two-dimensional electrophoresis Short title: Trimeric Ga protein solubilisation

  2007cites this paper
• Agonist-induced tyrosine phosphorylation of Gq/G11 alpha requires the intact structure of membrane domains.

  2005cites this paper
• Dominant portion of thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and Gqalpha/G11alpha protein.

  2005cites this paper
• Long-term agonist stimulation of IP prostanoid receptor depletes the cognate G(s)alpha protein in membrane domains but does not change the receptor level.

  2004cites this paper
• Biochemistry of transmembrane signaling mediated by trimeric G proteins.

  2004cites this paper
• Resolution of Gsα and Gqα/G11α proteins in membrane domains by two-dimensional electrophoresis: the effect of long-term agonist stimulation.

  2004cites this paper
• Resolution of G(s)alpha and G(q)alpha/G(11)alpha proteins in membrane domains by two-dimensional electrophoresis: the effect of long-term agonist stimulation.

  2004cites this paper
• δ‐Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains

  2003cites this paper
• Hormone-induced subcellular redistribution of trimeric G proteins

  2002cites this paper
• Mechanisms of regulation of G(11)alpha protein by dexamethasone in osteoblastic UMR 106-01 cells.

  2002cites this paper
• Mechanisms of regulation of G11α protein by dexamethasone in osteoblastic UMR 106–01 cells

  2002cites this paper
• A functional enhanced green fluorescent protein (EGFP)-tagged angiotensin II at(1a) receptor recruits the endogenous Galphaq/11 protein to the membrane and induces its specific internalization independently of receptor-g protein coupling in HEK-293 cells.

  2001cites this paper
• Agonist Regulation of D2 Dopamine Receptor/G Protein Interaction

  2001cites this paper
• Kinetic analysis of the internalization and recycling of [3H]TRH and C-terminal truncations of the long isoform of the rat thyrotropin-releasing hormone receptor-1.

  2000cites this paper
• Growth Hormone and Dexamethasone Stimulate Lipolysis and Activate Adenylyl Cyclase in Rat Adipocytes by Selectively Shifting Giα2 to Lower Density Membrane Fractions.

  1999cites this paper
• Thyrotropin-releasing hormone-induced depletion of G(q)alpha/G(11)alpha proteins from detergent-insensitive membrane domains.

  1999cites this paper
• Overexpression of the G protein G11alpha prevents desensitization of Ca2+ response to thyrotropin-releasing hormone.

  1999cites this paper
• Resolution and identification of Gq/G11alpha and Gialpha/Goalpha proteins in brown adipose tissue: effect of cold acclimation.

  1999cites this paper
• Activation of the β2-Adrenergic Receptor-Gαs Complex Leads to Rapid Depalmitoylation and Inhibition of Repalmitoylation of Both the Receptor and Gαs *

  1999cites this paper
• Agonist-induced translocation of Gq/11α immunoreactivity directly from plasma membrane in MDCK cells.

  1999cites this paper
• Visualization of Agonist-induced Association and Trafficking of Green Fluorescent Protein-tagged Forms of Both β-Arrestin-1 and the Thyrotropin-releasing Hormone Receptor-1*

  1999cites this paper
• Diversity in the signalling and regulation of G-protein-coupled receptors.

  1999cites this paper
• Thyrotropin‐releasing hormone‐induced depletion of Gqα/G11α proteins from detergent‐insensitive membrane domains

  1999cites this paper
• Resolution and identification of G q /G 11 Æ and G i Æ/G o Æ proteins in brown adipose tissue: eVect of cold acclimation

  1999cites this paper
• Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation.

  1999cites this paper
• New aspects of G-protein-coupled receptor signalling and regulation.

  1998cites this paper
• The long (Gs(alpha)-L) and short (Gs(alpha)-S) variants of the stimulatory guanine nucleotide-binding protein. Do they behave in an identical way?

  1998cites this paper
• Real Time Visualization of Agonist-mediated Redistribution and Internalization of a Green Fluorescent Protein-tagged Form of the Thyrotropin-releasing Hormone Receptor*

  1998cites this paper
• Protein kinase C does not mediate phenylephrine-induced down-regulation of Madin-Darby canine kidney cell alpha-1B adrenoceptors.

  1998cites this paper
• Agonist-induced Internalization of the G Protein G11α and Thyrotropin-releasing Hormone Receptors Proceed on Different Time Scales*

  1998cites this paper
• Interaction of the G-protein G11alpha with receptors and phosphoinositidase C: the contribution of G-protein palmitoylation and membrane association.

  1997cites this paper
• Interaction of the G‐protein G11α with receptors and phosphoinositidase C:

  1997cites this paper
• Inverse agonist-induced up-regulation of the human beta2-adrenoceptor in transfected neuroblastoma X glioma hybrid cells.

  1996cites this paper