A new lignan from the non-transformed root in vitro cultures of Phyllanthus amarus was isolated. The structure of the compound was established on the basis of one- and two-dimensional NMR, as well as mass spectrometry data, as 7'-oxocubebin dimethylether (1,4-bis(benzo[d][1,3]dioxol-5-yl)-2,3-bis(methoxymethyl)butan-1-on). The non-transformed root cultures of P. amarus showed to be a selective source of this compound. The lignan revealed strong cytotoxic activity against HeLa cell line with an IC50 value of 3.8 µg/mL.
Cytotoxic Lignan from the Non-Transformed Root Culture of Phyllanthus amarus
B. Sparzak,M. Krauze-Baranowska,A. Kawiak,P. Sowiński
Published 2015 in Molecules
ABSTRACT
PUBLICATION RECORD
- Publication year
2015
- Venue
Molecules
- Publication date
2015-04-30
- Fields of study
Biology, Medicine, Chemistry
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- 7'-oxocubebin dimethylether
A lignan compound isolated from the root cultures and structurally characterized in this paper.
Aliases: 1,4-bis(benzo[d][1,3]dioxol-5-yl)-2,3-bis(methoxymethyl)butan-1-on
- cytotoxic activity
Biological activity describing the compound's ability to inhibit or kill cultured cells in the assay reported here.
- hela cell line
A human cervical cancer cell line used in the cytotoxicity assay reported in the abstract.
Aliases: HeLa
- mass spectrometry
An analytical technique used here alongside NMR to support identification of the isolated lignan.
Aliases: MS
- non-transformed root in vitro cultures
In vitro root cultures of Phyllanthus amarus maintained without transformation and used as the plant material in this work.
Aliases: non-transformed root cultures
- one- and two-dimensional nmr
Nuclear magnetic resonance spectroscopic methods used here for structural elucidation of the isolated compound.
Aliases: NMR, 1D and 2D NMR
- phyllanthus amarus
The plant species whose non-transformed root in vitro cultures were investigated as a source of lignans.
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