Identification and conformational changes of the intestinal proline carrier.

Fluorescein isothiocyanate (FITC) was used to selectively label the rabbit intestinal brush-border imino carrier, identify the binding protein on SDS-polyacrylamide gel electrophoresis, and monitor the effect of ions on fluorescein quenching. FITC inhibits Na+-dependent L-proline transport irreversibly, but transport is protected by physiological concentrations of Na+ and L-proline. About 1 nmol of FITC/mg of protein binds specifically to the transporter, which was identified by SDS-polyacrylamide gel electrophoresis as a 100 +/- 5-kDa peptide. Na+ produced a specific, saturable quench in the fluorescence of FITC bound to the proline carrier. Both transport and FITC quenching are inhibited by n-acetylimidazole, and membranes are protected from acetylation by Na+. We conclude that Na+ binds to the proline carrier (100-kDa peptide) to produce a change in conformation that results in an increase in the affinity of the carrier for proline.

• Publication year

  1984

• Venue

  Journal of Biological Chemistry

• Publication date

  1984-12-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(17)42499-9PMID 6439715
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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