The C‐terminus of human glutaminase L mediates association with PDZ domain‐containing proteins 1

The enzyme glutaminase in brain is responsible for the synthesis of neurotransmitter glutamate. We used the two‐hybrid genetic selection system in yeast to look for interactors of glutaminase in human brain. We have identified two proteins containing PDZ domains, α1‐syntrophin and a glutaminase‐interacting protein, named GIP, that showed association with human glutaminase L, as deduced from specificity test of the two‐hybrid system. The complete GIP cDNA clone has 1315 nucleotides with a 372‐base open reading frame encoding a 124‐amino acids protein. Glutaminase associates with both PDZ proteins through its C‐terminal end; mutagenesis of single amino acids revealed the sequence ‐ESXV as essential for the interaction. These data suggest the possibility that PDZ domain‐containing proteins are involved in the regulation of glutaminase in brain.

• Publication year

  2001

• Venue

  FEBS Letters

• Publication date

  2001-01-19

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0014-5793(00)02373-5PMID 11163757
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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