Normalizing RT-qPCR Data: Are We Getting the Right Answers? An Appraisal of Normalization Approaches and Internal Reference Genes from a Case Study in the Finfish Lates calcarifer

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Marine Biotechnology

• Publication date

  2011-04-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1007/s10126-010-9277-zPMID 20309600
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.

  2009influential reference
• Expression of growth-related genes in muscle during fasting and refeeding of juvenile Atlantic halibut, Hippoglossus hippoglossus L.

  2009cited by this paper
• The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.

  2008cited by this paper
• Molecular characterization, tissue expression and sequence variability of the barramundi (Lates calcarifer) myostatin gene

  2008influential reference
• Stability of reference genes for real-time PCR analyses in channel catfish (Ictalurus punctatus) tissues under varying physiological conditions.

  2008influential reference
• Expression Profiling and Validation of Potential Reference Genes During Paralichthys olivaceus Embryogenesis

  2008cited by this paper
• Bmc Molecular Biology Selection of Housekeeping Genes for Gene Expression Studies in Larvae from Flatfish Using Real-time Pcr

  2008cited by this paper
• Candidate growth genes in finfish — Where should we be looking?

  2007cited by this paper
• Actin, a reliable marker of internal control?

  2007cited by this paper
• Appropriate 'housekeeping' genes for use in expression profiling the effects of environmental estrogens in fish

  2007influential reference
• Validation of Reference Genes for Real-Time Polymerase Chain Reaction Studies in Atlantic Salmon

  2006cited by this paper
• cDNA encoding sequences for myostatin and FGF6 in sea bass (Dicentrarchus labrax, L.) and the effect of fasting and refeeding on their abundance levels.

  2006influential reference
• Alterations in expression of genes associated with muscle metabolism and growth during nutritional restriction and refeeding in rainbow trout.

  2006influential reference
• Characterization of two parvalbumin genes and their association with growth traits in Asian seabass (Lates calcarifer).

  2006cited by this paper
• Effects of 28 day exposure to cold temperature or feed restriction on growth, body composition, and expression of genes related to muscle growth and metabolism in channel catfish

  2005cited by this paper
• PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR

  2004cited by this paper
• Regulation of muscle mass by myostatin.

  2004cited by this paper
• Growth performance, body composition and plasma thyroid hormone status of channel catfish (Ictalurus punctatus) in response to short-term feed deprivation and refeeding

  2004cited by this paper
• Compensatory growth in juvenile rainbow trout, Oncorhynchus mykiss (Walbaum), held individually

  2004cited by this paper
• Up‐regulation of muscle‐specific transcription factors during embryonic somitogenesis of zebrafish (Danio rerio) by knock‐down of myostatin‐1

  2004influential reference
• Prolonged fasting and cortisol reduce myostatin mRNA levels in tilapia larvae; short-term fasting elevates.

  2003cited by this paper
• Glucocorticoid-induced skeletal muscle atrophy is associated with upregulation of myostatin gene expression.

  2003cited by this paper
• A real-time polymerase chain reaction-based evaluation of cDNA synthesis priming methods.

  2003cited by this paper
• Effect of refeeding on IGFI, IGFII, IGF receptors, FGF2, FGF6, and myostatin mRNA expression in rainbow trout myotomal muscle.

  2003cited by this paper
• Compensatory growth in fishes: a response to growth depression

  2003cited by this paper
• Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

  2002influential reference
• Regulation of myostatin activity and muscle growth

  2001cited by this paper
• Rapid Cycle Real-Time PCR

  2001cited by this paper
• Quantification on the LightCycler

  2001cited by this paper
• A new mathematical model for relative quantification in real-time RT-PCR.

  2001cited by this paper
• Beta-actin--an unsuitable internal control for RT-PCR.

  2001cited by this paper
• Changes in the Expression of Genes Encoding Steroidogenic Enzymes in the Channel Catfish (Ictalurus punctatus) Ovary Throughout a Reproductive Cycle1

  2000cited by this paper
• Use of Compensatory Growth to Double Hybrid Sunfish Growth Rates

  1997cited by this paper
• How should the repeatability of clinical measurements be analysed? An assessment of analysis techniques with data from cardiovascular autonomic function tests.

  1993cited by this paper
• Basic local alignment search tool.

  1990cited by this paper

• Implantation of aromatase inhibitor fadrozole and 17β-estradiol antagonistically affect gonad development in the protandrous barramundi (Lates calcarifer)

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• Molecular forensics: RNA degradation as a marker for postmortem interval determination.

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  2022cites this paper
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  2022cites this paper
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  2021cites this paper
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  2021cites this paper
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  2020cites this paper
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  2020cites this paper
• Adequate supply of dietary taurine stimulates expression of molecular markers of growth and protein turnover in juvenile barramundi (Lates calcarifer)

  2020cites this paper
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  2020cites this paper
• Postprandial changes in plasma free amino acids and hepatic gene expression in response to variable methionine inclusion in the diet of barramundi ( Lates calcarifer ).

  2019cites this paper
• Assessment of Suitable Reference Genes for RT-qPCR Normalization with Developmental Samples in Pacific Abalone Haliotis discus hannai

  2019cites this paper
• Postprandial plasma free amino acid profile and hepatic gene expression in juvenile barramundi (Lates calcarifer) is more responsive to feed consumption than to dietary methionine inclusion

  2019cites this paper
• Sex-specific dmrt1 and cyp19a1 methylation and alternative splicing in gonads of the protandrous hermaphrodite barramundi

  2018cites this paper
• Characterization of lipid metabolism genes and the influence of fatty acid supplementation in the hepatic lipid metabolism of dusky grouper (Epinephelus marginatus).

  2018cites this paper
• Usual normalization strategies for gene expression studies impair the detection and analysis of circadian patterns

  2018cites this paper
• Analysis of Different Approaches for the Selection of Reference Genes in RT-qPCR Experiments: A Case Study in Skeletal Muscle of Growing Mice

  2017cites this paper
• Morphological changes and regulation of the genes dmrt1 and cyp11b during the sex differentiation of barramundi (Lates calcarifer Bloch)

  2017cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
• Identification of suitable reference genes in Taxodium ‘Zhongshanshan’ under abiotic stresses

  2017cites this paper
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  2016cites this paper
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• Identification of Sex and Female’s Reproductive Stage in Commercial Fish Species through the Quantification of Ribosomal Transcripts in Gonads

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  2016cites this paper
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  2016cites this paper
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  2016cites this paper
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  2016cites this paper
• Effects of canola meal on growth, feed utilisation, plasma biochemistry, histology of digestive organs and hepatic gene expression of barramundi (Asian seabass; Lates calcarifer)

  2016cites this paper
• Fatty acid metabolism in barramundi (Lates Calcarifer)

  2016cites this paper
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  2015cites this paper
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  2015cites this paper
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  2015cites this paper
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  2015cites this paper
• Evaluation of candidate reference genes for expression study in Saccharum spp. hybrids under heavy metal stress

  2015influential citation
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• Comprehensive Selection of Reference Genes for Gene Expression Normalization in Sugarcane by Real Time Quantitative RT-PCR

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  2014cites this paper
• Postprandial molecular responses in the liver of the barramundi, Lates calcarifer

  2014influential citation
• Persian sturgeon insulin-like growth factor I: molecular cloning and expression during various nutritional conditions

  2014cites this paper
• Identification and Validation of Different HouseKeeping Genes for real time RT-PCR in Plants

  2014cites this paper
• Inbred strains of zebrafish exhibit variation in growth performance and myostatin expression following fasting.

  2013cites this paper
• Gelatinases impart susceptibility to high-fat diet-induced obesity in mice.

  2013cites this paper
• Gene expression and activity of digestive enzymes during the larval development of Asian seabass (Lates calcarifer).

  2013cites this paper
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  2013cites this paper
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  2013cites this paper
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  2013cites this paper
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  2013cites this paper
• Otx2 expression and implications for olfactory imprinting in the anemonefish, Amphiprion percula

  2013cites this paper
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  2012cites this paper
• Expression of zona pellucida B proteins in juvenile rare minnow (Gobiocypris rarus) exposed to 17α-ethinylestradiol, 4-nonylphenol and bisphenol A.

  2012cites this paper
• Gene expression specificity of the mussel antifungal mytimycin (MytM).

  2012cites this paper
• Abundance of myostatin gene transcripts and their correlation with muscle hypertrophy during the development of barramundi, Lates calcarifer.

  2012cites this paper
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  2012cites this paper
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  2012cites this paper
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  2012cites this paper
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  2012cites this paper
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