Rearing Honey Bees, Apis mellifera, in vitro 1: Effects of Sugar Concentrations on Survival and Development

Abstract A new method for rearing honey bees, Apis mellifera L. (Hymenoptera: Apidae), in vitro was developed and the effects of sugar concentrations on survival and development were studied. Seven different glucose (G) and fructose (F) compositions (0%G+0%F, 3%G+3%F, 6%G+6%F, 12%G+12%F, 0%G+12%F, 12%G+0%F, and 4%G+8%F) were tested. Larvae were able to grow to the post defecation stage without addition of sugars (Diet 1), but they were not able to metamorphose and pupate. Adults were reared from diets 2–7. The average larval survival, prepupal larval weights, adult weights, and ovariole numbers were affected significantly due to the sugar compositions in the diets. High sugar concentrations (12%G+12%F) increased the number of queens and intercastes.

• Publication year

  2011

• Venue

  Journal of Insect Science

• Publication date

  2011-07-27

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology

• Identifiers
  DOI 10.1673/031.011.9601PMID 22208776PMCID 3391908
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Flight performance of artificially reared honeybees (Apis mellifera)

  2009cited by this paper
• Improvement of artificial feeding in a standard in vitro method for rearing Apis mellifera larvae

  2005cited by this paper
• A single gene (yes) controls pigmentation of eyes and scales in Heliothis virescens

  2001cited by this paper
• Effects of chlortetracycline of honey bee worker larvae reared

  1992cited by this paper
• Technique for rearing worker honeybees in the laboratory

  1987cited by this paper
• Behavioural and physiological aspects of nurse bees in relation to the composition of larval food during caste differentiation in the honeybee.

  1987cited by this paper
• An Artificial Diet for Laboratory Rearing of Honeybees

  1986cited by this paper
• Feeding behaviour of nurse bees, larval food composition and caste differentiation in the honey bee (Apis mellifera L.)

  1985cited by this paper
• Composition of freshly harvested and commercial royal jelly

  1985cited by this paper
• Glucose/fructose ratio in the food of honeybee larvae during caste differentiation

  1984cited by this paper
• Rearing of honeybee larvae in vitro: effect of yeast extract on queen differentiation

  1981influential reference
• Growth and Development of Honeybees in the Laboratory on Altered Queen and Worker Diets

  1978cited by this paper
• The effect of sugars and juvenile hormone on the differentiation of the female honeybee larvae (Apis mellifera L.) to queens.

  1976influential reference
• Control of Dimorphism in the Female Honeybee. 3. The Balance of Nutrients

  1974cited by this paper
• The chemical basis of honeybee, Apis mellifera, caste formation. Partial purification of queen bee determinator from royal jelly

  1974cited by this paper
• Control of Dimorphism in the Female Honeybee 2. Methods of Rearing Larvae in the Laboratory and of Preserving Royal Jelly

  1974cited by this paper
• Control of Dimorphism in the Female Honeybee. 4. Techniques for Fractionating Royal Jelly Into its Major Components

  1974cited by this paper
• Caste determination in honey bees. I. The significane of moisture in larval food.

  1971cited by this paper
• Caste Determination in Honey Bees. II. Food Consumption of Individual Honey Bee Larvae, Determined with 32P-Labeled Royal Jelly

  1970cited by this paper
• Honey Bee Nutrition

  1969influential reference
• Physiology of caste determination.

  1966cited by this paper
• Growth Rates of Young Queen and Worker Honeybee Larvae

  1965cited by this paper
• Biologically active substances in royal jelly.

  1965influential reference
• LABORATORY REARING STUDIES OF THE POSTCAPPING STAGES OF THE HONEY BEE (APIS MELLIFERA L.). I. REARING BROOD OUTSIDE CELLS.

  1965influential reference
• Egg-Weight Variance in Honey Bees

  1965cited by this paper
• STARVATION STUDIES OF LARVAL HONEY BEES

  1964cited by this paper
• Studies on Rearing Honey Bee Larvae in the Laboratory. I. The Effect of Royal Jelly Taken from Different Ages of Queen Cells on Queen Differentiation

  1964cited by this paper
• Egg Weight Variability and its Inheritance in the Honey Bee

  1963cited by this paper
• Rearing Worker Honeybee Larvae in an Incubator

  1960cited by this paper
• Annual Review of Entomology

  1960cited by this paper
• The Journal of Economic Entomology

  1957cited by this paper
• Rearing Honeybee Larvae on Royal Jelly in the Laboratory

  1955cited by this paper
• A Method of Rearing Honey Bee Larvae in vitro

  1955cited by this paper
• Rearing of Honeybee Larvae on Royal Jelly in the Laboratory.

  1955cited by this paper
• Ein Beitrag zur Frage der Arbeitsteilung im Bienenstaat

  1952cited by this paper
• Larval Food and Development of Castes in the Honeybee

  1943cited by this paper

• Substantial variation in larval honey bee nutrition within and among Apis mellifera colonies.

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• Social and nutritional factors controlling the growth of honey bee (Apis mellifera) queens

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• Eat to Reproduce: The Role of Diet Quantity on Honey Bee (Apis Mellifera) Caste Determination

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• Gene expression changes in honey bees induced by sublethal imidacloprid exposure during the larval stage.

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