An In Vitro System Comprising Immortalized Hypothalamic Neuronal Cells (GT1–7 Cells) for Evaluation of the Neuroendocrine Effects of Essential Oils

Aromatherapy and plant-based essential oils are widely used as complementary and alternative therapies for symptoms including anxiety. Furthermore, it was reportedly effective for the care of several diseases such as Alzheimer's disease and depressive illness. To investigate the pharmacological effects of essential oils, we developed an in vitro assay system using immortalized hypothalamic neuronal cells (GT1–7 cells). In this study, we evaluated the effects of essential oils on neuronal death induced by hydrogen peroxide (H2O2), aluminum, zinc, or the antagonist of estrogen receptor (tamoxifen). Among tests of various essential oils, we found that H2O2-induced neuronal death was attenuated by the essential oils of damask rose, eucalyptus, fennel, geranium, ginger, kabosu, mandarin, myrrh, and neroli. Damask rose oil had protective effects against aluminum-induced neurotoxicity, while geranium and rosemary oil showed protective activity against zinc-induced neurotoxicity. In contrast, geranium oil and ginger oil enhanced the neurotoxicity of tamoxifen. Our in vitro assay system could be useful for the neuropharmacological and endocrine pharmacological studies of essential oils.

• Publication year

  2015

• Venue

  Evidence-Based Complementary and Alternative Medicine

• Publication date

  2015-10-21

• Fields of study

  Medicine, Environmental Science

• Identifiers
  DOI 10.1155/2015/343942PMID 26576190PMCID 4631885
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2005influential reference
• Axonal transport of rubidium and thallium in the olfactory nerve of mice.

  2005cited by this paper
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  2002cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2000cited by this paper
• Estrogens stimulate tamoxifen-induced neuronal cell apoptosis in vitro: a possible nongenomic action.

  1997cited by this paper
• Fragrance compounds and essential oils with sedative effects upon inhalation.

  1993cited by this paper
• Immortalization of hypothalamic GnRH by genetically targeted tumorigenesis

  1990cited by this paper
• Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis.

  1990cited by this paper

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