Calcium ions function as a booster of chromosome condensation

Chromosome condensation is essential for the faithful transmission of genetic information to daughter cells during cell division. The depletion of chromosome scaffold proteins does not prevent chromosome condensation despite structural defects. This suggests that other factors contribute to condensation. Here we investigated the contribution of divalent cations, particularly Ca2+, to chromosome condensation in vitro and in vivo. Ca2+ depletion caused defects in proper mitotic progression, particularly in chromosome condensation after the breakdown of the nuclear envelope. Fluorescence lifetime imaging microscopy-Förster resonance energy transfer and electron microscopy demonstrated that chromosome condensation is influenced by Ca2+. Chromosomes had compact globular structures when exposed to Ca2+ and expanded fibrous structures without Ca2+. Therefore, we have clearly demonstrated a role for Ca2+ in the compaction of chromatin fibres.

• Publication year

  2016

• Venue

  Scientific Reports

• Publication date

  2016-12-02

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/srep38281PMID 27910894PMCID 5133622
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Shaping mitotic chromosomes: From classical concepts to molecular mechanisms

  2015cited by this paper
• Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins

  2015cited by this paper
• The role of phosphorylation of histone H3 at serine 10 in chromatin condensation in vitro

  2015cited by this paper
• A dark green fluorescent protein as an acceptor for measurement of Förster resonance energy transfer

  2015cited by this paper
• Chromosomes Progress to Metaphase in Multiple Discrete Steps via Global Compaction/Expansion Cycles.

  2015cited by this paper
• A Cascade of Histone Modifications Induces Chromatin Condensation in Mitosis

  2014cited by this paper
• Nuclear CaMKII enhances histone H3 phosphorylation and remodels chromatin during cardiac hypertrophy

  2013cited by this paper
• Condensins: universal organizers of chromosomes with diverse functions.

  2012cited by this paper
• Signaling pathways that regulate cell division.

  2012cited by this paper
• The polyelectrolyte properties of chromatin

  2012cited by this paper
• Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase IIα

  2012cited by this paper
• The divalent cations Ca2+ and Mg2+ play specific roles in stabilizing histone-DNA interactions within nucleosomes that are partially redundant with the core histone tail domains.

  2011cited by this paper
• Controlling the cell cycle: The role of calcium/calmodulin-stimulated protein kinases I and II

  2011cited by this paper
• Electrostatic origin of salt-induced nucleosome array compaction.

  2010cited by this paper
• Chromatin ionic atmosphere analyzed by a mesoscale electrostatic approach.

  2010cited by this paper
• New insight into the mitotic chromosome structure: irregular folding of nucleosome fibers without 30-nm chromatin structure.

  2010cited by this paper
• Quantitative analysis of chromatin compaction in living cells using FLIM–FRET

  2009cited by this paper
• The proteome of human metaphase chromosomes

  2009cited by this paper
• Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions

  2009cited by this paper
• The Middle Region of an HP1-binding Protein, HP1-BP74, Associates with Linker DNA at the Entry/Exit Site of Nucleosomal DNA*

  2009cited by this paper
• Histone Deacetylase 5 Acquires Calcium/Calmodulin-Dependent Kinase II Responsiveness by Oligomerization with Histone Deacetylase 4

  2008cited by this paper
• Coiled internal structure of chromonema within chromosomes suggesting hierarchical coil model for chromosome structure

  2008cited by this paper
• Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis.

  2008cited by this paper
• Chromosome protein framework from proteome analysis of isolated human metaphase chromosomes.

  2007cited by this paper
• Maximal chromosome compaction occurs by axial shortening in anaphase and depends on Aurora kinase

  2007cited by this paper
• Packaging the Genome: the Structure of Mitotic Chromosomes

  2007cited by this paper
• Detecting Protein‐Protein Interactions In Vivo with FRET using Multiphoton Fluorescence Lifetime Imaging Microscopy (FLIM)

  2007cited by this paper
• High resolution SIMS imaging of cations in mammalian cell mitosis, and in Drosophila polytene chromosomes

  2006cited by this paper
• Molecular analysis of mitotic chromosome condensation using a quantitative time-resolved fluorescence microscopy assay

  2006cited by this paper
• Highly compact folding of chromatin induced by cellular cation concentrations. Evidence from atomic force microscopy studies in aqueous solution

  2006cited by this paper
• Timing and checkpoints in the regulation of mitotic progression.

  2004cited by this paper
• CyclinB1 expression is elevated and mitosis is delayed in HeLa cells expressing autonomous CaMKII.

  2003cited by this paper
• Regulation of cell cycle progression by calcium/calmodulin-dependent pathways.

  2003cited by this paper
• Ca2+ signal blockers can inhibit M/A transition in mammalian cells by interfering with the spindle checkpoint.

  2003cited by this paper
• Emi1 is required for cytostatic factor arrest in vertebrate eggs

  2002cited by this paper
• Cation–chromatin binding as shown by ion microscopy is essential for the structural integrity of chromosomes

  2001cited by this paper
• Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome.

  1999cited by this paper
• Calcium/Calmodulin-dependent Phosphorylation and Activation of Human Cdc25-C at the G2/M Phase Transition in HeLa Cells*

  1999cited by this paper
• Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells.

  1998cited by this paper
• Crystal structure of the nucleosome core particle at 2.8 Å resolution

  1997cited by this paper
• Cell-cycle calcium transients driven by cyclic changes in inositol trisphosphate levels

  1994cited by this paper
• The role of calcium in cell division.

  1994cited by this paper
• Intracellular free calcium and mitosis in mammalian cells: anaphase onset is calcium modulated, but is not triggered by a brief transient

  1989cited by this paper
• Effect of microinjected calcium-calmodulin on mitosis in PtK2 cells.

  1987cited by this paper
• Specific Binding of Calcium to Soluble Chromatin

  1986cited by this paper
• The role of calcium ions during mitosis

  1983cited by this paper
• Architecture of metaphase chromosomes and chromosome scaffolds

  1983cited by this paper
• Metaphase chromosome structure: evidence for a radial loop model.

  1979cited by this paper
• Spheroid Chromatin Units (ν Bodies)

  1974cited by this paper
• Spheroid chromatin units (v bodies).

  1974cited by this paper

• Gene expression regulation by Ca2+ signaling: an updated systematic review.

  2026cites this paper
• Selective binding of divalent cations reshapes nucleosome mechanics and unlocks histone tail dynamics.

  2026cites this paper
• Nucleus softens during herpesvirus infection

  2026cites this paper
• Calcium waves and nuclear tension changes coordinate mechanical stress dissipation in locally folded epithelia

  2025cites this paper
• Where, When, and How? Integrating Spatiotemporal Cues in Cell Division

  2025cites this paper
• IVF and Thermal Manipulation at the First Cleavage Stage Alter Offspring Circadian Phenotype, Sleep, and Brain Epigenetics

  2025cites this paper
• Imaging and mechanism of DNA-DNA recognition mediated by divalent ions

  2025cites this paper
• Mechanosensitive and Reversible Chromatin-Lamina Dewetting Triggers Cellular Contraction During Wound Healing

  2025cites this paper
• Nuclear destabilisation – a possible genesis of cancer?

  2025cites this paper
• Structure and compaction of plant chromosomes: Studies using advanced electron microscopy.

  2025cites this paper
• Irregular Swelling of Tomato Fruit Chambers During the Early Stages of Fruit Formation is Due to the Local Distribution of Cell Wall-Loosening Proteins Under Ca-deficient Conditions

  2025cites this paper
• Nucleus softens during herpesvirus infection

  2025cites this paper
• Astroglial calcium signaling and homeostasis in tuberous sclerosis complex

  2024cites this paper
• Toxic Effects of Synthesized Bismuth Oxide/Reduced Graphene Oxide (Bi2O3/RGO) Nanocomposites in Two Distinct Mammalian Cell Lines: Role Oxidative Stress and Apoptosis

  2024cites this paper
• Depolarization induces calcium-dependent BMP4 release from mouse embryonic palate mesenchymal cells

  2024cites this paper
• Effect of Modified Bioceramic Mineral Trioxide Aggregate Cement with Mesoporous Nanoparticles on Human Gingival Fibroblasts

  2024cites this paper
• Physiology of intracellular calcium buffering

  2023cites this paper
• The restriction of calcium influx in metaphase and post‐metaphase stages of cell division revealed by imaging secondary ion mass spectrometry (SIMS)

  2023cites this paper
• The role of Ca2+-signaling in the regulation of epigenetic mechanisms.

  2023cites this paper
• Ion-mediated condensation controls the mechanics of mitotic chromosomes

  2023cites this paper
• Function and regulation of transcription factors during mitosis-to-G1 transition

  2022cites this paper
• 3D Ultrastructural Imaging of Chromosomes Using Serial Block-Face Scanning Electron Microscopy (SBFSEM)

  2022cites this paper
• COMPARISON OF SEMEN QULAITY FOR THREE LINES OF HOLSTEIN BULLS: 1. SOME IMMEDIATE AND MICROSCOPIC CHARACTERISTICS

  2022cites this paper
• Making Mitotic Chromosomes in a Test Tube

  2022cites this paper
• In vivo and in vitro performances of chitosan-coated Mg–Zn–Zr–Gd–Ca alloys as bone biodegradable materials in rat models

  2022cites this paper
• Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity is required for V(D)J recombination

  2021cites this paper
• Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure

  2021cites this paper
• Calcium ion significance on the maintenance of barley (Hordeum vulgare) chromosome compaction.

  2021cites this paper
• ATR prevents Ca2+ overload‐induced necrotic cell death through phosphorylation‐mediated inactivation of PARP1 without DNA damage signaling

  2021cites this paper
• Mitotic chromosomes

  2021cites this paper
• Mechanical Forces in Nuclear Organization.

  2021cites this paper
• Twisting DNA by salt

  2021cites this paper
• Imaging the inner structure of chromosomes: contribution of focused ion beam/scanning electron microscopy to chromosome research

  2021cites this paper
• The Winner Takes It All: Auxin—The Main Player during Plant Embryogenesis

  2020cites this paper
• Application of the Chromosome Image Analyzing System (CHIAS) for Straightening Cation‐treated Bent Chromosomes

  2020cites this paper
• A potent antagonist antibody targeting connexin hemichannels alleviates Clouston syndrome symptoms in mutant mice

  2020cites this paper
• Cold‐induced chromatin compaction and nuclear retention of clock mRNAs resets the circadian rhythm

  2020cites this paper
• DNA damage in paediatric obesity: a promoter and predictor of cancer in adulthood

  2020cites this paper
• Reversible Changes of Chromosome Structure upon Different Concentrations of Divalent Cations

  2019cites this paper
• First proteomic analysis of diestrus and anestrus canine oocytes at the germinal vesicle reveals candidate proteins involved in oocyte meiotic competence.

  2019cites this paper
• Cellular dynamics of mammalian red blood cell production in the erythroblastic island niche

  2019cites this paper
• Spermidine strongly increases the fidelity of Escherichia coli CRISPR Cas1–Cas2 integrase

  2019cites this paper
• Reorganisation of chromatin during erythroid differentiation

  2019cites this paper
• Nucleosome-level 3D organization of the genome.

  2018cites this paper
• Cdk1-dependent phosphorylation of KIF4A at S1186 triggers lateral chromosome compaction during early mitosis

  2018cites this paper
• The effect of linker DNA on the structure and interaction of nucleosome core particles.

  2018cites this paper
• Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes

  2018cites this paper
• Calcium Dynamics as a Machine for Decoding Signals.

  2018cites this paper
• A Transient Rise in Free Mg2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation.

  2018cites this paper
• Functional analysis of chromosomal proteins and cations on mitotic chromosome structure based on imaging techniques

  2018cites this paper
• High-Yield Method To Fabricate and Functionalize DNA Nanoparticles from the Products of Rolling Circle Amplification.

  2018cites this paper
• The interplay of nanointerface curvature and calcium binding in weak polyelectrolyte-coated nanoparticles.

  2018cites this paper
• Epigenome Interactions with Patterned Neuronal Activity

  2018cites this paper
• High-resolution studies of chromatin arrays and their salt-dependent compaction

  2017cites this paper
• Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ

  2017cites this paper
• Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore

  2017cites this paper
• Mitotic Chromosome Assembly In Vitro: Functional Cross Talk between Nucleosomes and Condensins.

  2017cites this paper
• Structural analysis of human chromosome by FIB/SEM

  2016cites this paper
• Digital Commons @ East Tennessee State University Digital Commons @ East Tennessee State University

  year unknowncites this paper