Analysis of somatic mutations in kappa transgenes

We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations identified were single base substitutions. Mutations were localized to the variable (V) region and its flanking sequences. In every case, the nuclear matrix association region, kappa enhancer, and C gene were spared. These data indicate that the rearranged kappa gene contains the necessary sequences for targeting of the mutation process, and suggest that the observed localization of mutations to the V region reflects the inherent specificity of this mutation process.

• Publication year

  1990

• Venue

  Journal of Experimental Medicine

• Publication date

  1990-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.172.1.131PMID 2358776PMCID 2188163
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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