THE CELLULAR SITE OF ACTION OF ANGIOTENSIN

The site of action and the distribution of angiotensin II have been studied in the mouse. A comparison of the ratios of angiotensin-14C and inulin-3H at the time of the pressor effect reveals an extracellular pattern of distribution. Morphological studies were made using angiotensin coupled to exogenous enzymes which can be demonstrated histochemically. Coupling of angiotensin to horseradish peroxidase or cytochrome c, with glutaraldehyde or difluorodinitrodiphenylsulfone (FNPS) as the coupling agent, does not alter the pattern of its vasopressor response or that of its inactivation; nor are differences present between angiotensin and the angiotensin-enzyme complexes in the stimulation of in vitro tissue preparations. Dissociation of the complexes was shown not to occur in vitro, but the possibility of a serum factor splitting the complexes immediately after intravenous injection cannot be excluded. Since these complexes are localized on the endothelium and not on the smooth muscle at the time of maximum hypertension, the endothelium is proposed as the site of action for angiotensin.

• Publication year

  1971

• Venue

  Journal of Cell Biology

• Publication date

  1971-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.51.2.419PMID 4329616PMCID 2108140
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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