Silk Fibroin Films for Corneal Endothelial Regeneration: Transplant in a Rabbit Descemet Membrane Endothelial Keratoplasty.

Purpose Develop a silk fibroin (SF)-based artificial endothelial graft for its use in a rabbit Descemet membrane endothelial keratoplasty (DMEK). Methods Human and rabbit artificial corneal endothelial grafts were developed through the culture of human and rabbit corneal endothelial cells (CECs) on SF films. Rabbit artificial SF endothelial grafts were transplanted in a DMEK surgery into a rabbit in vivo model. Results SF artificial endothelial grafts showed the characteristic endothelial markers: zonula occludens (ZO-1) and Na+/K+ ATPase. In a rabbit model of DMEK surgery, SF artificial endothelial graft restored the corneal transparency and thickness at 6 week of follow-up. Anterior segment optical coherence tomography revealed the SF graft as a fully integrated component in the corneal tissue, displaying a similar corneal thickness and endothelial cell count when compared with its healthy contralateral cornea. Histologic analysis showed that the SF artificial endothelial graft was attached and integrated on the surface of the corneal stroma without a significant inflammatory reaction, and rabbit CECs consisted in a monolayer that showed their characteristic markers ZO-1 and Na+/K+ ATPase, suggesting proper intercellular junctions and cellular pump function. Conclusions We have developed SF films with biological properties that supported the growth of rabbit and human CECs, which showed normal morphology and characteristic markers; and with mechanical properties that allowed its use in a DMEK surgery, proving its in vivo functionality in a rabbit model of endothelial dysfunction.

• Publication year

  2017

• Venue

  Investigative Ophthalmology and Visual Science

• Publication date

  2017-07-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1167/iovs.17-21797PMID 28687847
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Corneal Transplantation

  2024cited by this paper
• Human Bone Derived Collagen for the Development of an Artificial Corneal Endothelial Graft. In Vivo Results in a Rabbit Model

  2016cited by this paper
• Rho kinase inhibitor enables cell-based therapy for corneal endothelial dysfunction

  2016cited by this paper
• Descemet Membrane Endothelial Keratoplasty (DMEK)

  2016cited by this paper
• Characterization of a Novel Collagen Scaffold for Corneal Tissue Engineering

  2015cited by this paper
• Functional fabrication of recombinant human collagen-phosphorylcholine hydrogels for regenerative medicine applications.

  2015cited by this paper
• Optimizing outcomes with Descemet's membrane endothelial keratoplasty

  2014cited by this paper
• Development and evaluation of porcine atelocollagen vitrigel membrane with a spherical curve and transplantable artificial corneal endothelial grafts.

  2014cited by this paper
• Surgical strategies to improve visual outcomes in corneal transplantation

  2014cited by this paper
• Transplantation of tissue-engineered human corneal endothelium in cat models

  2013cited by this paper
• Descemet's membrane endothelial keratoplasty surgery: update on the evidence and hurdles to acceptance

  2013cited by this paper
• Keratin-chitosan membranes as scaffold for tissue engineering of human cornea.

  2013cited by this paper
• Ultrathin chitosan-poly(ethylene glycol) hydrogel films for corneal tissue engineering.

  2013cited by this paper
• Descemet membrane endothelial keratoplasty versus descemet stripping automated endothelial keratoplasty.

  2012cited by this paper
• Corneal transplantation.

  2012cited by this paper
• Biomechanics of the anterior human corneal tissue investigated with atomic force microscopy.

  2012cited by this paper
• Methods being developed for preparation, delivery and transplantation of a tissue-engineered corneal endothelium.

  2012cited by this paper
• ROCK inhibitor converts corneal endothelial cells into a phenotype capable of regenerating in vivo endothelial tissue.

  2012cited by this paper
• Visual performance with wave aberration correction after penetrating, deep anterior lamellar, or endothelial keratoplasty.

  2012cited by this paper
• Human corneal endothelial cell growth on a silk fibroin membrane.

  2011cited by this paper
• Endothelial Keratoplasty: Fellow Eyes Comparison of Descemet Stripping Automated Endothelial Keratoplasty and Descemet Membrane Endothelial Keratoplasty

  2011cited by this paper
• Graft thickness, graft folds, and aberrations after descemet stripping endothelial keratoplasty for fuchs dystrophy.

  2011cited by this paper
• Descemet's membrane endothelial keratoplasty: prospective study of 1-year visual outcomes, graft survival, and endothelial cell loss.

  2011cited by this paper
• Advancing Towards a Tissue-engineered Tympanic Membrane: Silk Fibroin as a Substratum for Growing Human Eardrum Keratinocytes

  2010cited by this paper
• Corneal higher-order aberrations after Descemet's stripping automated endothelial keratoplasty.

  2010cited by this paper
• Endothelial keratoplasty – a review

  2010cited by this paper
• Human Anterior Lens Capsule as Carrier Matrix for Cultivated Human Corneal Endothelial Cells

  2009cited by this paper
• Cultivated Corneal Endothelial Transplantation in a Primate: Possible Future Clinical Application in Corneal Endothelial Regenerative Medicine

  2008cited by this paper
• Bombyx mori Silk Fibroin Membranes as Potential Substrata for Epithelial Constructs Used in the Management of Ocular Surface Disorders

  2008cited by this paper
• Silk as a Biomaterial.

  2007cited by this paper
• Cultivated corneal endothelial cell sheet transplantation in a primate model.

  2007cited by this paper
• Tissue-Engineered Human Corneal Endothelial Cell Sheet Transplantation in a Rabbit Model Using Functional Biomaterials

  2007cited by this paper
• Silk as Substratum for Cell Attachment and Proliferation

  2007cited by this paper
• Stem cell-based tissue engineering with silk biomaterials.

  2006cited by this paper
• The inflammatory responses to silk films in vitro and in vivo.

  2005cited by this paper
• Long-term outcome of iron-endocytosing cultured corneal endothelial cell transplantation with magnetic attraction.

  2005cited by this paper
• Water‐Stable Silk Films with Reduced β‐Sheet Content

  2005cited by this paper
• Human corneal endothelial cell precursors isolated by sphere-forming assay.

  2005cited by this paper
• Transplantation of corneas reconstructed with cultured adult human corneal endothelial cells in nude rats.

  2004cited by this paper
• Cultured human corneal endothelial cell transplantation with a collagen sheet in a rabbit model.

  2004cited by this paper
• Amniotic membrane as a carrier for cultivated human corneal endothelial cell transplantation.

  2004cited by this paper
• Biodegradation of Bombyx mori silk fibroin fibers and films

  2004cited by this paper
• Prospects for endothelial transplantation.

  2004cited by this paper
• Long-term graft survival after penetrating keratoplasty.

  2003cited by this paper
• Macrophage responses to silk.

  2003cited by this paper
• Magnetic attraction of iron-endocytosed corneal endothelial cells to Descemet's membrane.

  2003cited by this paper
• Regenerated silk fibroin films: Thermal and dynamic mechanical analysis

  2002cited by this paper
• Transplantation of Descemet's Membrane Carrying Viable Endothelium Through a Small Scleral Incision

  2002cited by this paper
• Materials: Surprising strength of silkworm silk

  2002cited by this paper
• Transplantation of Adult Human Corneal Endothelium Ex Vivo: A Morphologic Study

  2001cited by this paper
• Suture-related Complications Following Keratoplasty: A 5-Year Retrospective Study

  2001cited by this paper
• In Vitro Culture Characteristics of Corneal Epithelial, Endothelial, and Keratocyte Cells in a Native Collagen Matrix

  2000cited by this paper
• Cell cycle kinetics in corneal endothelium from old and young donors.

  2000cited by this paper
• Preoperative risk factors for surface disease after penetrating keratoplasty.

  1997cited by this paper
• Suprachoroidal hemorrhage in penetrating keratoplasty.

  1994cited by this paper
• Corneal endothelial cell transplantation using Descemet's membrane as a carrier

  1993cited by this paper
• Expulsive hemorrhage in penetrating keratoplasty.

  1982cited by this paper
• Morphology of transplanted corneal endothelium derived from tissue culture.

  1981cited by this paper
• Corneal epithelium in penetrating keratoplasty.

  1980cited by this paper
• A gelatin membrane substrate for the transplantation of tissue cultured cells.

  1980cited by this paper
• Corneal endothelial transplantation.

  1980cited by this paper
• Bacterial endophthalmitis following suture removal after penetrating keratoplasty.

  1975cited by this paper

• Silk Fibroin Sheets Improve the Strength of Colon Anastomoses in Wistar Rats

  2026cites this paper
• Silk Fibroin in Medicine: A Review on ISO10993 Biocompatibility, Preclinical Evidence, and Clinical Perspectives

  2026cites this paper
• Advances in Corneal Tissue Engineering: Comparative Performance of Bioengineered Grafts in Animal Models

  2025cites this paper
• Pathogenesis and treatment: research progress on corneal endothelial decompensation

  2025cites this paper
• Thermoplastic silk-plasticizer membranes for biodegradable artificial corneal endothelium.

  2025cites this paper
• Human Omental Mesothelial Cells Exhibit a Corneal Endothelial-Like Cell Phenotype for Tissue Engineering of a Corneal Endothelium Biomimetic

  2025cites this paper
• Application of silk fibroin-based composite films in biomedicine and biotechnology.

  2025cites this paper
• Silk biomaterials for corneal tissue engineering: From research approaches to therapeutic potentials; A review.

  2025cites this paper
• Silk Fibroin-Based Materials for Tissue Engineering

  2025cites this paper
• Exploring Single-cell RNA sequencing as a decision-making tool in the clinical management of Fuchs' Endothelial Corneal Dystrophy.

  2024cites this paper
• Advancements in Polymer Biomaterials as Scaffolds for Corneal Endothelium Tissue Engineering

  2024cites this paper
• Проблема получения клеточной культуры эндотелиальных клеток роговицы для регенеративных целей

  2024cites this paper
• Alginate Hydrogel Integrated with a Human Fibroblast-Derived Extracellular Matrix Supports Corneal Endothelial Cell Functionality and Suppresses Endothelial-Mesenchymal Transition.

  2024cites this paper
• In Vitro Profiling of the Extracellular Matrix and Integrins Expressed by Human Corneal Endothelial Cells Cultured on Silk Fibroin-Based Matrices.

  2023cites this paper
• Development of Thermoplastic Polyurethane Films for the Replacement of Corneal Endothelial Function of Transparency Maintenance.

  2023cites this paper
• An effective method for culturing functional human corneal endothelial cells using a xenogeneic free culture medium

  2023cites this paper
• Biomaterials used for tissue engineering of barrier-forming cell monolayers in the eye

  2023cites this paper
• Medizinische Ansätze zur Regeneration des Hornhautendothels

  2023cites this paper
• Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

  2023cites this paper
• Novel Corneal Endothelial Cell Carrier Couples a Biodegradable Polymer and a Mesenchymal Stem Cell-Derived Extracellular Matrix.

  2022cites this paper
• Promising Role of Silk-Based Biomaterials for Ocular-Based Drug Delivery and Tissue Engineering

  2022cites this paper
• Corneal endothelial transplantation from bench to bedside: A review of animal models and their translational value for therapeutic development.

  2022cites this paper
• Modification and release of calcitonin gene-related peptide on silk fibroin film

  2022cites this paper
• Use of Bombyx mori silk fibroin in tissue engineering: From cocoons to medical devices, challenges, and future perspectives.

  2022cites this paper
• Comparative culture of human corneal endothelial cells following treatment with human platelet lysate/fibrin hydrogel versus Y-27632 ROCK inhibitor: in vitro and ex vivo study

  2022cites this paper
• Potential of a novel scaffold composed of human platelet lysate and fibrin for human corneal endothelial cells

  2021cites this paper
• The Human Tissue-Engineered Cornea (hTEC): Recent Progress

  2021cites this paper
• Biomaterials for corneal endothelial cell culture and tissue engineering

  2021cites this paper
• Current development of alternative treatments for endothelial decompensation: Cell-based therapy.

  2021cites this paper
• Approaches for corneal endothelium regenerative medicine.

  2021cites this paper
• Recent Advances in Natural Materials for Corneal Tissue Engineering

  2021cites this paper
• Use of biomaterials in corneal endothelial repair

  2021cites this paper
• Tissue engineered endothelial keratoplasty in rabbit: tips and tricks

  2021cites this paper
• Designing Scaffolds for Corneal Regeneration

  2020cites this paper
• The silk of gorse spider mite Tetranychus lintearius represents a novel natural source of nanoparticles and biomaterials

  2020cites this paper
• Experimental design of a culture approach for corneal endothelial cells of New Zealand white rabbit

  2020cites this paper
• Corneal stromal regeneration by hybrid oriented poly (ε-caprolactone)/lyophilized silk fibroin electrospun scaffold.

  2020cites this paper
• Recombinant Spider Silk Forms Tough and Elastic Nanomembranes that are Protein‐Permeable and Support Cell Attachment and Growth

  2020cites this paper
• Reduced graphene oxide membranes in ocular regenerative medicine.

  2020cites this paper
• Biosynthetic alternatives for corneal transplant surgery

  2020cites this paper
• In Vitro Culture of Human Corneal Endothelium on Non-Mulberry Silk Fibroin Films for Tissue Regeneration

  2020cites this paper
• New developments in corneal endothelial cell replacement

  2020cites this paper
• Silk fibroin scaffolds seeded with Wharton’s jelly mesenchymal stem cells enhance re-epithelialization and reduce formation of scar tissue after cutaneous wound healing

  2019cites this paper
• Dysfunctional Corneal Endothelium: Delivery of Cell Therapy

  2019cites this paper
• Corneal Endothelial Cell Transplantation: Animal Models

  2019cites this paper
• Corneal Endothelium Regeneration: Future Prospects

  2019cites this paper
• In Vitro Evaluation and Transplantation of Human Corneal Endothelial Cells Cultured on Biocompatible Carriers

  2019cites this paper
• Corneal Endothelial Cells Over the Past Decade: Are We Missing the Mark(er)?

  2019cites this paper
• Experimental models of corneal endothelial cell therapy and translational challenges to clinical practice.

  2019cites this paper
• Poly-ε-lysine based hydrogels as synthetic substrates for the expansion of corneal endothelial cells for transplantation

  2019cites this paper
• Corneal Repair and Regeneration: Current Concepts and Future Directions

  2019cites this paper
• Investigating the effect of chitosan/ polycaprolactone blends in differentiation of corneal endothelial cells and extracellular matrix compositions.

  2019cites this paper
• Effect of different cocoon stifling methods on the properties of silk fibroin biomaterials

  2019cites this paper
• Characterization of Human iPSC-RPE on a Prosthetic Bruch's Membrane Manufactured From Silk Fibroin

  2018cites this paper
• Biofunctionalized Lysophosphatidic Acid/Silk Fibroin Film for Cornea Endothelial Cell Regeneration

  2018cites this paper
• UNIVERSITY OF CALIFORNIA, MERCED Mechanical Signaling and Patterning Stem Cell-derived Vascular Cells A dissertation submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy in Materials and Biomaterials Science Engineering by

  2018cites this paper
• A Review of the Emerging Role of Silk for the Treatment of the Eye

  2018influential citation
• The present and the prospect of bioengineering cornea

  2018cites this paper
• Vascular Cell Co-Culture on Silk Fibroin Matrix

  2018cites this paper
• Ex Vivo Functionality of 3D Bioprinted Corneal Endothelium Engineered with Ribonuclease 5‐Overexpressing Human Corneal Endothelial Cells

  2018cites this paper
• Biomaterials for Regenerative Medicine Approaches for the Anterior Segment of the Eye

  2018cites this paper