Structural characterization of an iron–sulfur cluster assembly protein IscU in a zinc‐bound form

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Proteins: Structure, Function, and Bioinformatics

• Publication date

  2005-06-01

• Fields of study

  Biology, Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1002/prot.20421PMID 15815978
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Solution NMR structure of the iron-sulfur cluster assembly protein U (IscU) with zinc bound at the active site.

  2004cited by this paper
• Bacterial IscU is a well folded and functional single domain protein.

  2004cited by this paper
• Contributions of the LPPVK Motif of the Iron-Sulfur Template Protein IscU to Interactions with the Hsc66-Hsc20 Chaperone System*

  2003influential reference
• Thermotoga maritima IscU. Structural characterization and dynamics of a new class of metallochaperone.

  2003cited by this paper
• Cys-328 of IscS and Cys-63 of IscU are the sites of disulfide bridge formation in a covalently bound IscS/IscU complex: Implications for the mechanism of iron-sulfur cluster assembly

  2002influential reference
• Iron-Sulfur Cluster Biosynthesis

  2002cited by this paper
• Iron-Sulfur Cluster Assembly CHARACTERIZATION OF IscA AND EVIDENCE FOR A SPECIFIC AND FUNCTIONAL COMPLEX WITH FERREDOXIN*

  2001cited by this paper
• Iron-Sulfur Cluster Assembly

  2001cited by this paper
• Sulfur transfer from IscS to IscU: the first step in iron-sulfur cluster biosynthesis.

  2001cited by this paper
• A Mutant Human IscU Protein Contains a Stable [2Fe−2S]2+ Center of Possible Functional Significance

  2000influential reference
• Biogenesis of iron-sulfur proteins in eukaryotes: a novel task of mitochondria that is inherited from bacteria.

  2000cited by this paper
• Iron-sulfur proteins: ancient structures, still full of surprises

  2000cited by this paper
• IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU.

  2000cited by this paper
• Distinct iron–sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells

  2000cited by this paper
• Modular organization and identification of a mononuclear iron-binding site within the NifU protein

  2000cited by this paper
• Transfer of Iron-Sulfur Cluster from NifU to Apoferredoxin*

  2000cited by this paper
• Automated MAD and MIR structure solution

  1999cited by this paper
• Saccharomyces cerevisiae ISU1 and ISU2: members of a well-conserved gene family for iron-sulfur cluster assembly.

  1999cited by this paper
• Assembly of Iron-Sulfur Clusters

  1998cited by this paper
• [20] Processing of X-ray diffraction data collected in oscillation mode.

  1997cited by this paper
• Processing of X-ray diffraction data collected in oscillation mode.

  1997cited by this paper
• Methods used in the structure determination of bovine mitochondrial F1 ATPase.

  1996cited by this paper
• A modular domain of NifU, a nitrogen fixation cluster protein, is highly conserved in evolution

  1996cited by this paper
• The CCP4 suite: programs for protein crystallography.

  1994cited by this paper
• Protein structure comparison by alignment of distance matrices.

  1993cited by this paper
• Sparse matrix sampling: a screening method for crystallization of proteins

  1991cited by this paper
• Improved methods for building protein models in electron density maps and the location of errors in these models.

  1991cited by this paper
• Ligation-independent cloning of PCR products (LIC-PCR).

  1990cited by this paper

• Mechanism of mitochondrial [2Fe2S] cluster biosynthesis.

  2024cites this paper
• Light-driven biodegradation of chloramphenicol by photosensitized Shewanella oneidensis MR-1.

  2024cites this paper
• The reduced interaction between SufS and SufU in Mycoplasma penetrans results in diminished sulfotransferase activity.

  2024cites this paper
• Native mass spectrometric studies of IscSU reveal a concerted, sulfur-initiated mechanism of iron–sulfur cluster assembly

  2022cites this paper
• Structural diversity of cysteine desulfurases involved in iron-sulfur cluster biosynthesis

  2022cites this paper
• Characteristics of the Isu1 C-terminus in relation to [2Fe-2S] cluster assembly and ISCU Myopathy

  2022cites this paper
• Protein interactions in the biological assembly of iron–sulfur clusters in Escherichia coli : Molecular and mechanistic aspects of the earliest assembly steps

  2022cites this paper
• Mechanistic Insights into IscU Conformation Regulation for Fe-S Cluster Biogenesis Revealed by Variable Temperature Electrospray Ionization Native Ion Mobility Mass Spectrometry.

  2022cites this paper
• Molecular Details of the Frataxin–Scaffold Interaction during Mitochondrial Fe–S Cluster Assembly

  2021cites this paper
• Mechanism of Iron–Sulfur Cluster Assembly: In the Intimacy of Iron and Sulfur Encounter

  2020cites this paper
• Unique roles of iron and zinc binding to the yeast Fe-S cluster scaffold assembly protein "Isu1".

  2019cites this paper
• Physiologically relevant reconstitution of iron-sulfur cluster biosynthesis uncovers persulfide-processing functions of ferredoxin-2 and frataxin

  2019cites this paper
• Zinc(II) binding on human wild-type ISCU and Met140 variants modulates Fe-S complex activity

  2018cites this paper
• Comparison of the Response of Bacterial IscU and SufU to Zn2+ and Select Transition-Metal Ions.

  2018cites this paper
• Zinc(II) binding on human wild-type ISCU and Met140 variants modulates NFS1 desulfurase activity

  2018cites this paper
• Zinc-Ligand Swapping Mediated Complex Formation and Sulfur Transfer between SufS and SufU for Iron-Sulfur Cluster Biogenesis in Bacillus subtilis.

  2017cites this paper
• In Vitro Studies of Cellular Iron-Sulfur Cluster Biosynthesis, Trafficking, and Transport.

  2017cites this paper
• New Techniques for Ancient Proteins: Direct Coupling Analysis Applied on Proteins Involved in Iron Sulfur Cluster Biogenesis

  2017cites this paper
• Myopathy with Deficiency of ISCU

  2016cites this paper
• An Overview on Fe–S Protein Biogenesis from Prokaryotes to Eukaryotes

  2015cites this paper
• Tangled web of interactions among proteins involved in iron-sulfur cluster assembly as unraveled by NMR, SAXS, chemical crosslinking, and functional studies.

  2015influential citation
• Anatomy of an iron-sulfur cluster scaffold protein: Understanding the determinants of [2Fe-2S] cluster stability on IscU.

  2015cites this paper
• Human Frataxin Activates Fe–S Cluster Biosynthesis by Facilitating Sulfur Transfer Chemistry

  2014cites this paper
• Insights into the Function of YciM, a Heat Shock Membrane Protein Required To Maintain Envelope Integrity in Escherichia coli

  2014cites this paper
• Fe-S cluster biogenesis in Gram-positive bacteria: SufU is a zinc-dependent sulfur transfer protein.

  2014cites this paper
• The role of zinc in the stability of the marginally stable IscU scaffold protein

  2014cites this paper
• coli Maintain Envelope Integrity in Escherichia Shock Membrane Protein Required To Insights into the Function of YciM , a Heat

  2013cites this paper
• Human Mitochondrial Chaperone (mtHSP70) and Cysteine Desulfurase (NFS1) Bind Preferentially to the Disordered Conformation, Whereas Co-chaperone (HSC20) Binds to the Structured Conformation of the Iron-Sulfur Cluster Scaffold Protein (ISCU)*

  2013cites this paper
• Iron–Sulfur Cluster Biogenesis in Archaea and Bacteria

  2013cites this paper
• Iron-Sulfur Centers: New Roles for Ancient Metal Sites

  2013cites this paper
• (IscS-IscU)2 complex structures provide insights into Fe2S2 biogenesis and transfer.

  2012cites this paper
• Three-dimensional structure and determinants of stability of the iron-sulfur cluster scaffold protein IscU from Escherichia coli.

  2012cites this paper
• Functions and properties of IscU-type and Nfu-type proteins in the biogenesis of iron-sulfur clusters

  2012influential citation
• Charakterisierung der essentiellen Eisen-Schwefel-Cluster Biosynthesekomponenten des Suf-Systems in Bacillus subtilis

  2011cites this paper
• Genetic and functional studies of hereditary myopathy with lactic acidosis

  2011cites this paper
• Facilitated transfer of IscU-[2Fe2S] clusters by chaperone-mediated ligand exchange.

  2011cites this paper
• Caracterização da maquinaria SUF responsável pela formação e associação dos cofatores [Fe-S] em Enterococcus faecalis

  2011influential citation
• Molecular details of the yeast frataxin-Isu1 interaction during mitochondrial Fe-S cluster assembly.

  2010cites this paper
• Copper Stress Affects Iron Homeostasis by Destabilizing Iron-Sulfur Cluster Formation in Bacillus subtilis

  2010cites this paper
• Structural studies of the Enterococcus faecalis SufU [Fe-S] cluster protein

  2009influential citation
• Iron-sulfur cluster biosynthesis: characterization of a molten globule domain in human NFU.

  2009cites this paper
• Function and biogenesis of iron–sulphur proteins

  2009cites this paper
• Augmenter of liver regeneration: substrate specificity of a flavin-dependent oxidoreductase from the mitochondrial intermembrane space.

  2009cites this paper
• Cluster Assembly Pathways in Bacteria

  2008cites this paper
• Splice mutation in the iron-sulfur cluster scaffold protein ISCU causes myopathy with exercise intolerance.

  2008cites this paper
• Glu43 is an essential residue for coordinating the [Fe2S2] cluster of IscR from Acidithiobacillus ferrooxidans

  2008cites this paper
• Fe-S Cluster Assembly Pathways in Bacteria

  2008cites this paper
• Iron-sulfur cluster biosynthesis.

  2008cites this paper
• The asymmetric trimeric architecture of [2Fe-2S] IscU: implications for its scaffolding during iron-sulfur cluster biosynthesis.

  2008cites this paper
• Structure of Human J-type Co-chaperone HscB Reveals a Tetracysteine Metal-binding Domain*

  2008cites this paper
• Asp97 is a crucial residue involved in the ligation of the [Fe4S4] cluster of IscA from Acidithiobacillus ferrooxidans.

  2008cites this paper
• Mapping iron binding sites on human frataxin: implications for cluster assembly on the ISU Fe–S cluster scaffold protein

  2008cites this paper
• Iron-sulfur cluster biosynthesis in bacteria: Mechanisms of cluster assembly and transfer.

  2008cites this paper
• The IscA from Acidithiobacillus ferrooxidans is an iron-sulfur protein which assemble the [Fe4S4] cluster with intracellular iron and sulfur.

  2007cites this paper
• Characterization and crystallization of an IscU-type scaffold protein with bound [2Fe-2S] cluster from the hyperthermophile, aquifex aeolicus.

  2007cites this paper
• Expression, purification and characterization of an iron-sulfur cluster assembly protein, IscU, from Acidithiobacillus ferrooxidans

  2007cites this paper
• In vitro activation of apo-aconitase using a [4Fe-4S] cluster-loaded form of the IscU [Fe-S] cluster scaffolding protein.

  2007cites this paper
• Formation and properties of [4Fe-4S] clusters on the IscU scaffold protein.

  2007cites this paper
• HscA and HscB stimulate [2Fe-2S] cluster transfer from IscU to apoferredoxin in an ATP-dependent reaction.

  2006cites this paper
• Identification of Zinc-ligated Cysteine Residues Based on 13Cα and 13Cβ Chemical Shift Data

  2006cites this paper
• Identification of zinc-ligated cysteine residues based on 13Calpha and 13Cbeta chemical shift data.

  2006cites this paper
• The Structure and Function of Frataxin

  2006influential citation
• Structural characterization of an iron–sulfur cluster assembly protein IscU in a zinc‐bound form

  2006cites this paper