Long-chain fatty acid transport in Escherichia coli. Cloning, mapping, and expression of the fadL gene.

Transport of long-chain fatty acids across the inner membrane of Escherichia coli K-12 requires a functional fadL gene (Maloy, S. R., Ginsburgh, C. L., Simons, R. W., and Nunn, W. D. (1981) J. Biol. Chem. 256, 3735-3742). Mutants defective in the fadL gene lack a 33,000-dalton inner membrane protein as evaluated using two-dimensional pI/sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (Ginsburgh, C. L., Black, P. N., and Nunn, W. D. (1984) J. Biol. Chem. 259, 8437-8443). In an effort to determine whether the fadL gene is the structural gene for this 33,000-dalton protein, we have cloned, mapped, and analyzed the expression of the fadL gene. The fadL gene has been localized on a 2.8-kilobase EcoRV fragment of E. coli genomic DNA. Plasmids containing this gene (i) complement all fadL mutants, (ii) increase the long-chain fatty acid transport activity of fadL strains harboring them by 2- to 3-fold, and (iii) direct the synthesis of a membrane protein which has the same molecular weight and isoelectric point as that described by Ginsburgh et al. This is a heat-modifiable protein which has an apparent molecular weight of 43,000 daltons when solubilized at 100 degrees C in the presence of SDS and 33,000 daltons when solubilized at 50 degrees C in the presence of SDS.

• Publication year

  1985

• Venue

  Journal of Biological Chemistry

• Publication date

  1985-02-10

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0021-9258(18)89661-2PMID 3881440
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular cloning of pldA, the structural gene for outer membrane phospholipase of E. coli K12

  2004cited by this paper
• Cloned structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12

  2004cited by this paper
• Transport of long chain fatty acids in Escherichia coli. Identification of a membrane protein associated with the fadL gene.

  1984cited by this paper
• Cloning, mapping, and expression of genes involved in the fatty acid-degradative multienzyme complex of Escherichia coli

  1984cited by this paper
• sn-Glycerol-3-phosphate transport in Salmonella typhimurium

  1983cited by this paper
• Regulation of fatty acid transport in Escherichia coli: analysis by operon fusion

  1983cited by this paper
• Cytodifferentiation in the accessory glands of Tenebrio molitor. VIII. Crossed immunoelectrophoretic analysis of terminal differentiation in the postecdysial tubular accessory glands.

  1982cited by this paper
• Selection for loss of tetracycline resistance by Escherichia coli

  1981cited by this paper
• Mechanism of long chain fatty acid permeation in the isolated adipocyte.

  1981cited by this paper
• Purification and characterization of acyl coenzyme A synthetase from Escherichia coli.

  1981cited by this paper
• Specific-purpose plasmid cloning vectors. I. Low copy number, temperature-sensitive, mobilization-defective pSC101-derived containment vectors.

  1981cited by this paper
• Transport of long and medium chain fatty acids by Escherichia coli K12.

  1981cited by this paper
• Rapid and efficient cosmid cloning

  1981cited by this paper
• Uptake and metabolism of fatty acids by dispersed adult rat heart myocytes. I. Kinetics of homologous fatty acids.

  1980cited by this paper
• Uptake and metabolism of fatty acids by dispersed adult rat heart myocytes. II. Inhibition by albumin and fatty acid homologues, and the effect of temperature and metabolic reagents.

  1980cited by this paper
• Regulation of fatty acid degradation in Escherichia coli: isolation and characterization of strains bearing insertion and temperature-sensitive mutations in gene fadR

  1980cited by this paper
• Linkage map of Escherichia coli K-12, edition 6.

  1980cited by this paper
• Regulation of fatty acid degradation in Escherichia coli: dominance studies with strains merodiploid in gene fadR

  1980cited by this paper
• Simple method for identification of plasmid-coded proteins

  1979cited by this paper
• Cloning of the structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12.

  1979cited by this paper
• Kinetics of the utilization of medium and long chain fatty acids by mutant of Escherichia coli defective in the fadL gene.

  1979cited by this paper
• A dual, concentration-dependent absorption mechanism of linoleic acid by rat jejunum in vitro.

  1979cited by this paper
• Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

  1979cited by this paper
• Transport of long-chain fatty acids by Escherichia coli: mapping and characterization of mutants in the fadL gene.

  1978cited by this paper
• Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

  1978cited by this paper
• Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

  1977cited by this paper
• Effects of heating in dodecyl sulfate solution on the conformation and electrophoretic mobility of isolated major outer membrane proteins from Escherichia coli K-12.

  1976cited by this paper
• Two-dimensional gel electrophoresis of membrane proteins.

  1976cited by this paper
• Uptake and metabolism of fatty acids and analogues by cultured cardiac cells from chick embryo.

  1976cited by this paper
• Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.

  1975cited by this paper
• A simple method for the preparation of large quantities of pure plasmid DNA.

  1975cited by this paper
• High resolution two-dimensional electrophoresis of proteins.

  1975cited by this paper
• Analysis of Endonuclease R·EcoRI Fragments of DNA from Lambdoid Bacteriophages and Other Viruses by Agarose-Gel Electrophoresis

  1974cited by this paper
• Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants.

  1971cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• Degradation of Exogenous Fatty Acids in Escherichia coli

  2022cites this paper
• A revolution in biochemistry and molecular biology education informed by basic research to meet the demands of 21st century career paths

  2021cites this paper
• Aryl Coenzyme A Ligases, a Subfamily of the Adenylate-Forming Enzyme Superfamily

  2021cites this paper
• Gonococcal adaptation to palmitic acid through farAB expression and FadD activity mutations increases in vivo fitness in a murine genital tract infection model.

  2020cites this paper
• A Novel Butanol Tolerance-Promoting Function of the Transcription Factor Rob in Escherichia coli

  2020cites this paper
• A revolution in biochemistry and molecular biology education informed by basic research to meet the demands of 21st century career paths

  2020cites this paper
• Incorporación de alcanos por bacterias degradadoras de hidrocarburos mediada por una proteína de 70 KDA unida a membranas durante la biodegradación de combustible diesel

  2013cites this paper
• The Acyl-Acyl Carrier Protein Synthetase from Synechocystis sp. PCC 6803 Mediates Fatty Acid Import1[C][W][OA]

  2012cites this paper
• Myristoylcoa:Protein N‐Myristoyltransferase

  2006cites this paper
• Tissue-cell- and species-specific expression of gonadotropin-regulated long chain acyl-CoA synthetase (GR-LACS) in gonads, adrenal and brain. Identification of novel forms in the brain.

  2006cites this paper
• Incorporation of Exogenous Docosahexaenoic Acid into Triacylglycerols and Polar Lipids of Chlorella vulgaris

  2005cites this paper
• The gonadotropin-regulated long-chain acyl CoA synthetase gene: a novel downstream Sp1/Sp3 binding element critical for transcriptional promoter activity.

  2005cites this paper
• Differential Protein Expression during Growth of Acidithiobacillus ferrooxidans on Ferrous Iron, Sulfur Compounds, or Metal Sulfides

  2004cites this paper
• Escherichia coli—a model system that benefits from and contributes to the evolution of proteomics

  2003cites this paper
• Escherichia coli—A Model System That Benefits From and Contributes to the Evolution of Proteomics

  2003cites this paper
• In vitro identification of two adherence factors required for in vivo virulence of Pseudomonas fluorescens.

  2003cites this paper
• Transmembrane Movement of Exogenous Long-Chain Fatty Acids: Proteins, Enzymes, and Vectorial Esterification

  2003cites this paper
• Skeletal muscle fatty acid transport and transporters.

  2001cites this paper
• Fatty acid transport proteins: a current view of a growing family.

  2001cites this paper
• Characterization of P1-deficient isogenic mutant of Haemophilus influenzae biogroup aegyptius associated with Brazilian purpuric fever.

  2000cites this paper
• Fatty Acid Competition as a Mechanism by Which Enterobacter cloacae Suppresses Pythium ultimum Sporangium Germination and Damping-Off

  2000cites this paper
• Localization of adipocyte long-chain fatty acyl-CoA synthetase at the plasma membrane.

  1999cites this paper
• Molecular inroads into the regulation and metabolism of fatty acids, lessons from bacteria.

  1999cites this paper
• Skeletal Muscle Metabolism in Exercise and Diabetes

  1998cites this paper
• Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map

  1998cites this paper
• Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map

  1998cites this paper
• Medium- and long-chain fatty acid uptake and utilization by Streptomyces coelicolor A3(2): first characterization of a gram-positive bacterial system.

  1997cites this paper
• Acyl Coenzyme a Synthetase and the Transport of Long-Chain Fatty Acids

  1996cites this paper
• Protein-Mediated Facilitated Uptake Processes for Fatty Acids, Bilirubin, and Other Amphipathic Compounds

  1996cites this paper
• Incorporation and turnover of fatty acids in Escherichia coli membrane phospholipids

  1996cites this paper
• Membrane permeation and intracellular trafficking of long chain fatty acids: insights from Escherichia coli and 3T3-L1 adipocytes.

  1995cites this paper
• Incorporation of exogenous docosahexaenoic acid into various bacterial phospholipids

  1994cites this paper
• Molecular and biochemical analyses of fatty acid transport, metabolism, and gene regulation in Escherichia coli.

  1994cites this paper
• Use of transposon TnphoA to identify genes for cell envelope proteins of Escherichia coli required for long-chain fatty acid transport: the periplasmic protein Tsp potentiates long-chain fatty acid transport

  1994cites this paper
• Bacterial long-chain fatty acid transport. Identification of amino acid residues within the outer membrane protein FadL required for activity.

  1993cites this paper
• Fatty acid uptake in Escherichia coli: regulation by recruitment of fatty acyl-CoA synthetase to the plasma membrane.

  1993cites this paper
• Osmoregulation of the fatty acid receptor gene fadL in Escherichia coli

  1993cites this paper
• Regulation of transcription of genes required for fatty acid transport and unsaturated fatty acid biosynthesis in Escherichiacoli by FadR

  1993cites this paper
• The Kinetics of Fatty Acid Uptake By Paramecium Tetraurelia

  1993cites this paper
• Cloning, sequencing, and expression of the fadD gene of Escherichia coli encoding acyl coenzyme A synthetase.

  1992cites this paper
• Stable, conserved outer membrane epitope of strains of Haemophilus influenzae biogroup aegyptius associated with Brazilian purpuric fever

  1992cites this paper
• Linker mutagenesis of a bacterial fatty acid transport protein. Identification of domains with functional importance.

  1991cites this paper
• Primary sequence of the Escherichia coli fadL gene encoding an outer membrane protein required for long-chain fatty acid transport

  1991cites this paper
• Linkage map of Escherichia coli K-12, edition 8

  1990cites this paper
• Mechanism for binding of fatty acids to hepatocyte plasma membranes: Different interpretation

  1990cites this paper
• Characterization of FadL-specific fatty acid binding in Escherichia coli.

  1990cites this paper
• Elongation of exogenous fatty acids by the bioluminescent bacterium Vibrio harveyi

  1989cites this paper
• Uptake and utilization of lipids by Paramecium tetraurelia.

  1989cites this paper
• Biosynthesis, processing and half-life of P-glycoprotein in a human multidrug-resistant KB cell.

  1989cites this paper
• Nucleotide sequencing and expression of the fadL gene involved in long‐chain fatty acid transport in Escherichia coli

  1988cites this paper
• Nucleotide sequence of the fadR gene, a multifunctional regulator of fatty acid metabolism in Escherichia coli.

  1988cites this paper
• The cellular fatty acid binding proteins: aspects of structure, regulation, and function.

  1988cites this paper
• Electrophoretic analysis of P-glycoproteins produced by mouse J774.2 and Chinese hamster ovary multidrug-resistant cells.

  1988cites this paper
• The fadL gene product of Escherichia coli is an outer membrane protein required for uptake of long-chain fatty acids and involved in sensitivity to bacteriophage T2

  1988cites this paper
• Flow microcalorimetry investigation of the influence of surfactants on a heterogeneous aerobic culture

  1987cites this paper
• Role of plasma membrane ligand‐binding proteins in the hepatocellular uptake of albumin‐bound organic anions

  1987cites this paper
• Genetic and molecular characterization of the genes involved in short-chain fatty acid degradation in Escherichia coli: the ato system

  1987cites this paper
• Purification and characterization of an outer membrane-bound protein involved in long-chain fatty acid transport in Escherichia coli.

  1987cites this paper
• A Model for Studying Membrane Fatty Acid Transport : Acyl-Coenzyme a Synthesis in Human Erythrocyte Ghosts

  1986cites this paper
• Molecular genetics of membrane phospholipid synthesis.

  1986cites this paper
• Transport of long-chain fatty acids in Escherichia coli. Evidence for role of fadL gene product as long-chain fatty acid receptor.

  1986cites this paper
• A molecular view of fatty acid catabolism in Escherichia coli

  1986cites this paper
• New locus (ttr) in Escherichia coli K-12 affecting sensitivity to bacteriophage T2 and growth on oleate as the sole carbon source

  1986cites this paper
• A molecular view of fatty acid catabolism in Escherichia coli.

  1986cites this paper
• Pathways for the incorporation of exogenous fatty acids into phosphatidylethanolamine in Escherichia coli.

  1985cites this paper
• A Molecular View of Fatty Acid Catabolism in Escherichia coli

  year unknowncites this paper
• Use of Transposon TnphoA To Identify Genes for Cell Envelope Proteins of Escherichia coli Required for Long-Chain Fatty Acid Transport : the Periplasmic Protein Tsp Potentiates Long-Chain Fatty Acid Transport

  year unknowncites this paper