Nitrogen Metabolism in Lignifying Pinus taeda Cell Cultures (*)

The primary metabolic fate of phenylalanine, following its deamination in plants, is conscription of its carbon skeleton for lignin, suberin, flavonoid, and related metabolite formation. Since this accounts for 30-40% of all organic carbon, an effective means of recycling the liberated ammonium ion must be operative. In order to establish how this occurs, the uptake and metabolism of various N-labeled precursors (N-Phe, NHCl, N-Gln, and N-Glu) in lignifying Pinus taeda cell cultures was investigated, using a combination of high performance liquid chromatography, N NMR, and gas chromatography-mass spectrometry analyses. It was found that the ammonium ion released during active phenylpropanoid metabolism was not made available for general amino acid/protein synthesis. Rather it was rapidly recycled back to regenerate phenylalanine, thereby providing an effective means of maintaining active phenylpropanoid metabolism with no additional nitrogen requirement. These results strongly suggest that, in lignifying cells, ammonium ion reassimilation is tightly compartmentalized.

• Publication year

  1996

• Venue

  Journal of Biological Chemistry

• Publication date

  1996-05-24

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1074/jbc.271.21.12350PMID 8647837
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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