Immunolabelling of fish host molecules on the tegumental surface of Ligula intestinalis (Cestoda: Ppseudophyllidea)

Immunoblotting, SDS-PAGE and western biotting procedures were used to demonstrate cross-reactivity of a polyclonal anti-carp IgM antibody with components of roach serum. The polyclonal antibody labelled 2 major bands in both immune and normal roach sera corresponding to molecular mannes of approximately 90 and 65 kDa. One of these bands (65 kDa) was considered to be the heavy chain of fish immunoglobulin whilst the identity of the other remains uncertain. This cross-reaction has been exploited in immunofluorescence and immunogold labelling studies to localize fish host molecules on the tegumental membrane of Ligula intestinalis freshly removed from roach fry. Immunogold studies revealed a low level of host molecules to be associated mainly with the microthrix spines of the tegament with less gold labelling being observed on the microthrix shafts.

• Publication year

  1995

• Venue

  International Journal of Parasitology

• Publication date

  1995-02-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0020-7519(94)00076-ZPMID 7622332PMCID 7130229
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Localization of glycoconjugates at the tegument of the tapeworms Hymenolepis nana and H. microstoma with gold labelled lectins

  2004cited by this paper
• Antibody response of the fish Rutilus rutilus to the metacestode of Ligula intestinalis

  1992cited by this paper
• Immunity to helminths.

  1989cited by this paper
• Cytotoxic reactions of rainbow trout, Salmo gairdneri Richardson, macrophages for larvae of the eye fluke Diplostomum spathaceum (Digenea)

  1989cited by this paper
• Ligula intestinalis (L.) (Cestoda: Pseudophyllidea): plerocercoid-induced changes in the spleen and pronephros of roach, Rutilus rutilus (L.), and gudgeon, Gobio gobio (L.)

  1989cited by this paper
• The immune response of wild rainbow trout, Salmo gairdneri Richardson, to naturally acquired plerocercoid infections of Diphyllobothrium dendriticum (Nitzsch, 1824) and D. ditremum (Creplin, 1825)

  1989cited by this paper
• Ligula intestinalis (Cestoda: Pseudophyllidea): phosphorylcholine inhibition of fish leucocyte adherence.

  1988cited by this paper
• Excretory–secretory products of helminth parasites: effects on host immune responses

  1988cited by this paper
• The role of serum in leucocyte adherence to the plerocercoid of Ligula intestinalis (Cestoda: Pseudophyllidea)

  1986cited by this paper
• The in vitro culture and tegumental dynamics of the plerocercoid of Ligula intestinalis (Cestoda: Pseudophyllidea)

  1985cited by this paper
• Host tissue response for trout infected with Diphyllobothrium cordiceps larvae

  1984cited by this paper
• Cell-mediated damage to helminths.

  1984cited by this paper
• Purification of a major serum protein of rainbow trout (Salmo gairdneri) homologous to the third component of mammalian complement.

  1984cited by this paper
• The immune response in immunized and naturally infected rainbow trout (Salmo gairdneri) to Diplostomum spathaceum as detected by enzyme-linked immunosorbent assay (ELISA).

  1984cited by this paper
• Structural and functional analysis of spontaneous anti-nitrophenyl antibodies in three cyprinid fish species: carp (Cyrinus carpio), goldfish (Carassius auratus) and tench (Tinca tinca).

  1984cited by this paper
• EXPERIMENTAL INFECTIONS OF RAINBOW TROUT, SALMO GAIRDNERI RICHARDSON, WITH PLEROCERCOIDS OF TRIAENOPHORUS CRASSUS FOREL

  1984cited by this paper
• Ultrastructural studies on the cellular response of fish hosts following experimental infection with the plerocercoid of Ligula intestinalis (Cestoda: Pseudophyllidea)

  1983cited by this paper
• Ligula intestinalis (Cestoda: Pseudophyllidea): An ultrastructural study on the cellular response of roach fry, Rutilus rutilus

  1983cited by this paper
• Ultrastructural studies on the cellular response of roach, Rutilus rutilus L., to the plerocercoid larva of the pseudophyllidean cestode, Ligula intestinalis

  1982cited by this paper
• Spirometra mansonoides: lectin analysis of tegumental glycopeptides.

  1982cited by this paper
• The complement system in rainbow trout (Salmo gairdneri). II. Purification and characterization of the fifth component (C5).

  1981cited by this paper
• Isolation of a phosphorylcholine‐containing component from the turbot tapeworm, Bothriocephalus scorpii (Müller), and its reaction with C‐reactive protein

  1980cited by this paper
• Interaction between Taenia taeniaeformis and the complement system.

  1978cited by this paper
• Hymenolepis diminuta and H. microstoma: mouse immunoglobulins binding to the tegumental surface.

  1977cited by this paper
• Studies on Ligula intestinalis Some aspects of the pathology in the second intermediate host

  1977cited by this paper
• Ascaris suum: location of phosphorylcholine in lung larvae.

  1977cited by this paper
• Phosphorylcholine‐bearing components in homogenates of nematodes

  1974cited by this paper
• TRIAENOPHORIASIS IN LAKE ERIE WHITE BASS, Morone chrysops1

  1974cited by this paper
• Cytological studies on the absorptive surfaces of cestodes. V. Incorporation of carbohydrate-containing macromolecules into tegument membranes.

  1971cited by this paper
• Observations on a tissue response within the body cavity of fish infected with the plerocercoid larvae of Ligula intestinalis (L.) (Cestoda: Pseudophyllidea)

  1970cited by this paper
• Occurrence and pathology of Ligula intestinalis infections in British fishes.

  1968cited by this paper
• [Demonstration of parasite-specific antibodies in fish blood by means of the agar-gel precipitation test].

  1965cited by this paper
• Animal body fluids and their regulation

  1964cited by this paper
• Cytological studies.

  1958cited by this paper
• Immunity to helminths.

  1958cited by this paper

• Structural Mechanisms of Protection of Parasitic Larvae from the Host’s Immune Factors in the Triaenophorus nodulosus (Cestoda)–Perch System

  2024cites this paper
• Структурные механизмы защиты паразитических личинок от иммунных факторов хозяина в системе Triaenophorus nodulosus (Cestoda)–окунь

  2024cites this paper
• Host-Associated Genetic Differentiation in the Face of Ongoing Gene Flow: Ecological Speciation in a Pathogenic Parasite of Freshwater Fish

  2024cites this paper
• The anthelmintic efficacy of natural plant cysteine proteinases against two rodent cestodes Hymenolepis diminuta and Hymenolepis microstoma in vitro.

  2014cites this paper
• Host specificity and adaptation of Schistocephalus to its stickleback hosts

  2014cites this paper
• Excretory products of the cestode, Schistocephalus solidus, modulate in vitro responses of leukocytes from its specific host, the three-spined stickleback (Gasterosteus aculeatus).

  2013cites this paper
• Immune response in spirlins (Alburnoides bipunctatus, Bloch 1782) infested by Ligula intestinalis parasite.

  2013cites this paper
• Determination of the electrophoretic pattern of somatic and excretory-secretory proteins of Ligula intestinalis parasite in spirlin (Alburnoides bipunctatus).

  2012cites this paper
• Antiparasitic Secondary Metabolites from Marine Actinobacteria

  2012cites this paper
• Ligula intestinalis (Cestoda: Pseudophyllidea): an ideal fish-metazoan parasite model?

  2010cites this paper
• Expression of gonadotropin subunits in roach (Rutilus rutilus, Cyprinidae) infected with plerocercoids of the tapeworm Ligula intestinalis (Cestoda).

  2009cites this paper
• Effects of a natural parasitical infection (Lernanthropus kroyeri) on the immune system of European sea bass, Dicentrarchus labrax L

  2009cites this paper
• Immune response of fish to eukaryotic parasites.

  2008cites this paper
• Living off a fish: a trade-off between parasites and the immune system.

  2008cites this paper
• Construction and screening of a cDNA library from the triactinomyxon spores of Myxobolus cerebralis, the causative agent of salmonid Whirling Diseases

  2006cites this paper
• Interaction between the blood fluke, Sanguinicola inermis and humoral components of the immune response of carp, Cyprinus carpio

  2005cites this paper