Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts

We investigated the effects of natural plant hormones, indole-3-acetic (IAA) acid and gibberellic acid (GA), on the growth parameters and production of flavonoids and other phenolic compounds in common buckwheat sprouts. A total of 17 phenolic compounds were identified using liquid chromatography-mass spectrometry (LC-MS) analysis. Among these, seven compounds (4-hydroxybenzoic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, rutin, and quercetin) were quantified by high-performance liquid chromatography (HPLC) after treating the common buckwheat sprouts with different concentrations of the hormones IAA and GA. At a concentration of 0.5 mg/L, both IAA and GA exhibited the highest levels of growth parameters (shoot length, root length, and fresh weight). The HPLC analysis showed that the treatment of sprouts with IAA at concentrations ranging from 0.1 to 1.0 mg/L produced higher or comparable levels of the total phenolic compounds than the control sprout and enhanced the production of rutin. Similarly, the supplementation with 0.1 and 0.5 mg/L GA increased the content of rutin in buckwheat sprouts. Our results suggested that the treatment with optimal concentrations of IAA and GA enhanced the growth parameters and accumulation of flavonoids and other phenolic compounds in buckwheat sprouts.

• Publication year

  2017

• Venue

  Molecules

• Publication date

  2017-02-28

• Fields of study

  Agricultural and Food Sciences, Biology, Chemistry, Environmental Science, Medicine

• Identifiers
  DOI 10.3390/molecules22030374PMID 28264513PMCID 6155264
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• LC-MS identified 17 phenolic compounds, and HPLC was used to quantify seven selected compounds in the sprouts.

  Confidence 0.93

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• Gibberellic acid at 0.1 and 0.5 mg/L increased rutin content in common buckwheat sprouts.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• Indole-3-acetic acid at 0.1 to 1.0 mg/L yielded total phenolic compound levels that were higher than or comparable to the control and increased rutin production in common buckwheat sprouts.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• At 0.5 mg/L, indole-3-acetic acid and gibberellic acid produced the highest shoot length, root length, and fresh weight in common buckwheat sprouts.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review

• common buckwheat sprouts

  plant material, biological sample

  Young germinated seedlings of Fagopyrum esculentum used as the experimental plant material.

  Aliases: buckwheat sprouts, Fagopyrum esculentum sprouts

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• gibberellic acid

  hormone, plant hormone

  A natural plant growth hormone used to treat buckwheat sprouts at different concentrations.

  Aliases: GA

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• growth parameters

  measurement, outcome

  Measured sprout growth traits including shoot length, root length, and fresh weight.

  Aliases: shoot length, root length, fresh weight

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• hplc

  analytical method, instrumental method

  High-performance liquid chromatography used to quantify selected phenolic compounds in the sprout extracts.

  Aliases: high-performance liquid chromatography

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• indole-3-acetic acid

  hormone, plant hormone

  A natural plant auxin hormone applied to buckwheat sprouts in graded concentrations.

  Aliases: IAA

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• lc-ms

  analytical method, instrumental method

  Liquid chromatography-mass spectrometry used to identify phenolic constituents in the sprout extracts.

  Aliases: liquid chromatography-mass spectrometry

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• phenolic compounds

  compound class, metabolite class

  A class of plant secondary metabolites present in the sprouts and profiled in the chemical analysis.

  Aliases: phenolics, total phenolic compounds

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• rutin

  compound, flavonoid

  A flavonoid compound quantified among the sprout phenolics.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review

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