Optogenetic control of human neurons in organotypic brain cultures

Optogenetics is one of the most powerful tools in neuroscience, allowing for selective control of specific neuronal populations in the brain of experimental animals, including mammals. We report, for the first time, the application of optogenetic tools to human brain tissue providing a proof-of-concept for the use of optogenetics in neuromodulation of human cortical and hippocampal neurons as a possible tool to explore network mechanisms and develop future therapeutic strategies.

• Publication year

  2016

• Venue

  Scientific Reports

• Publication date

  2016-04-21

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1038/srep24818PMID 27098488PMCID 4838935
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Differential Effect of Neuropeptides on Excitatory Synaptic Transmission in Human Epileptic Hippocampus

  2015cited by this paper
• Optogenetic and electrical microstimulation systematically bias visuospatial choice in primates.

  2014cited by this paper
• Optogenetic approaches for investigating neural pathways implicated in schizophrenia and related disorders.

  2014cited by this paper
• Optogenetics Reveal Delayed Afferent Synaptogenesis on Grafted Human‐Induced Pluripotent Stem Cell‐Derived Neural Progenitors

  2014cited by this paper
• Progress in understanding mood disorders: optogenetic dissection of neural circuits

  2014cited by this paper
• An organotypic brain slice preparation from adult patients with temporal lobe epilepsy.

  2014cited by this paper
• Optogenetic Stimulation of C1 and Retrotrapezoid Nucleus Neurons Causes Sleep State–Dependent Cardiorespiratory Stimulation and Arousal in Rats

  2013cited by this paper
• Creating a False Memory in the Hippocampus

  2013cited by this paper
• An optogenetic approach in epilepsy.

  2013cited by this paper
• Optogenetics in primates: a shining future?

  2013cited by this paper
• Optogenetic Probing of Fast Glutamatergic Transmission from Hypocretin/Orexin to Histamine Neurons In Situ

  2012cited by this paper
• Optogenetic stimulation of a hippocampal engram activates fear memory recall

  2012cited by this paper
• A High-Light Sensitivity Optical Neural Silencer: Development and Application to Optogenetic Control of Non-Human Primate Cortex

  2010cited by this paper
• Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures

  2010cited by this paper
• Optogenetic Deconstruction of Sleep–Wake Circuitry in the Brain

  2009cited by this paper
• Neural substrates of awakening probed with optogenetic control of hypocretin neurons

  2007cited by this paper
• Carbamazepine-resistance in the epileptic dentate gyrus of human hippocampal slices.

  2006cited by this paper
• Tonic GABAA receptor‐mediated currents in human brain

  2006cited by this paper
• Mouse Hippocampal Organotypic Tissue Cultures Exposed to In Vitro “Ischemia” Show Selective and Delayed CA1 Damage that is Aggravated by Glucose

  2003cited by this paper
• Channelrhodopsin-2, a directly light-gated cation-selective membrane channel

  2003cited by this paper
• Cells in human postmortem brain tissue slices remain alive for several weeks in culture

  2002cited by this paper

• Channeling Seizure Control: Optogenetics in Human Brain Slices

  2025cites this paper
• Organotypic models of the mammalian brain for molecular psychiatry and neurology

  2025cites this paper
• Bright Yellow-Emitting Carbon Dots via Microwave-assisted Synthesis for Rapid On-Site Cr(VI) Monitoring in Aquatic Environments

  2025cites this paper
• Review of the research progress of Precision Photostimulation for Optogenetics

  2025cites this paper
• Gene therapy for epilepsy: An emerging, promising approach for a serious neurological disorder

  2025cites this paper
• Illuminating minds vs. chemical control: a comprehensive comparison of optogenetics and chemogenetics in neuromodulation

  2025cites this paper
• Exploring the singularity of human neurons: keep calm and carry on

  2025cites this paper
• What makes the human brain special - from cellular function to clinical translation.

  2025cites this paper
• RNA-programmable cell-type monitoring and manipulation in the human cortex with CellREADR

  2025cites this paper
• Divergent actions of physiological and pathological amyloid-β on synapses in live human brain slice cultures

  2025cites this paper
• Opposing roles of physiological and pathological amyloid-β on synapses in live human brain slice cultures

  2024cites this paper
• p-tau Ser356 is associated with Alzheimer’s disease pathology and is lowered in brain slice cultures using the NUAK inhibitor WZ4003

  2024cites this paper
• RNA-programmable cell type monitoring and manipulation in the human cortex with CellREADR

  2024cites this paper
• Multimodal evaluation of network activity and optogenetic interventions in human hippocampal slices

  2024cites this paper
• Transport-Related Effects on Intrinsic and Synaptic Properties of Human Cortical Neurons: A Comparative Study

  2024cites this paper
• Metadynamics simulations reveal mechanisms of Na+ and Ca2+ transport in two open states of the channelrhodopsin chimera, C1C2

  2024cites this paper
• Human post-mortem organotypic brain slice cultures: a tool to study pathomechanisms and test therapies

  2024cites this paper
• In vitro human cell culture models in a bench‐to‐bedside approach to epilepsy

  2024cites this paper
• Human organotypic brain slice cultures: a detailed and improved protocol for preparation and long-term maintenance.

  2024cites this paper
• Combinatorial gene therapy for epilepsy: Gene sequence positioning and AAV serotype influence expression and inhibitory effect on seizures

  2023cites this paper
• Oligodendrocytes in human induced pluripotent stem cell-derived cortical grafts remyelinate adult rat and human cortical neurons

  2023cites this paper
• Long-term human organotypic brain slice cultures: a detailed protocol to provide a comprehensive framework for single-neuron and neuronal network investigations

  2023cites this paper
• Tau phosphorylated at serine 356 is associated with Alzheimer’s disease pathology and can be lowered in mouse and human brain tissue using the NUAK inhibitor WZ4003

  2023cites this paper
• Target cell-specific synaptic dynamics of excitatory to inhibitory neuron connections in supragranular layers of human neocortex

  2023cites this paper
• Network models to enhance the translational impact of cross-species studies

  2023cites this paper
• Electrophysiological and morphological characterization of single neurons in intact human brain organoids.

  2023cites this paper
• Technical report: surgical preparation of human brain tissue for clinical and basic research

  2023cites this paper
• GluK2 is a target for gene therapy in drug-resistant Temporal Lobe Epilepsy

  2023influential citation
• Animal models and human tissue compared to better understand and treat the epilepsies

  2023cites this paper
• Evaluation of advances in cortical development using model systems

  2022cites this paper
• Routine culture and study of adult human brain cells from neurosurgical specimens

  2022cites this paper
• Towards translational optogenetics

  2022cites this paper
• Modeling the Human Brain With ex vivo Slices and in vitro Organoids for Translational Neuroscience

  2022cites this paper
• Target cell-specific synaptic dynamics of excitatory to inhibitory neuron connections in supragranular layers of human neocortex

  2022cites this paper
• Riding brain "waves" to identify human memory genes.

  2022cites this paper
• Cross Talk proposal: Human‐derived brain tissue is a better epilepsy model than animal‐based approaches

  2022cites this paper
• A benchtop brain injury model using resected donor tissue from patients with Chiari malformation

  2022cites this paper
• Closed-loop optogenetic control of the dynamics of neural activity in non-human primates

  2022cites this paper
• Shaping the future of European epilepsy research: Final meeting report from EPICLUSTER.

  2022cites this paper
• Advancing models of neural development with biomaterials

  2021cites this paper
• Human stem cell-derived GABAergic neurons functionally integrate into human neuronal networks

  2021cites this paper
• Neural Infection by Oropouche Virus in Adult Human Brain Slices Induces an Inflammatory and Toxic Response

  2021cites this paper
• Spatiotemporal Correlation of Epileptiform Activity and Gene Expression in vitro

  2021cites this paper
• Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex

  2021cites this paper
• Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neuronal Cells.

  2021cites this paper
• The Concept of an Epilepsy Brain Bank

  2020cites this paper
• Current ex Vivo and in Vitro Approaches to Uncovering Mechanisms of Neurological Dysfunction after Traumatic Brain Injury.

  2020cites this paper
• A Bridge Between in vitro and in vivo Studies in Neuroscience: Organotypic Brain Slice Cultures.

  2020cites this paper
• Human in vitro systems for examining synaptic function and plasticity in the brain.

  2020cites this paper
• Closed-loop optogenetic control of normal and pathological network dynamics

  2020cites this paper
• Molecular and genetic approaches for assaying human cell type synaptic 1 connectivity 2 3

  2020cites this paper
• Optogenetics in Brain Research: From a Strategy to Investigate Physiological Function to a Therapeutic Tool

  2019cites this paper
• An optimized and automated approach to quantifying channelrhodopsin photocurrent kinetics.

  2019cites this paper
• Long-term adult human brain slice cultures as a model system to study human CNS circuitry and disease

  2019cites this paper
• On the Use of Optogenetics Wireless Systems in Modern Pathology: Opportunities and Challenges

  2019cites this paper
• Inhibition of epileptiform activity by neuropeptide Y in brain tissue from drug-resistant temporal lobe epilepsy patients

  2019cites this paper
• Does the maturation of optogenetics enable new approaches to the treatment of Parkinson's Disease?

  2019cites this paper
• Human Brain Slice Culture: A Useful Tool to Study Brain Disorders and Potential Therapeutic Compounds

  2019cites this paper
• Organotypic brain slice cultures to model neurodegenerative proteinopathies

  2019cites this paper
• Organotypic Brain Slices of ADULT Transgenic Mice: A Tool to Study Alzheimer's Disease.

  2019cites this paper
• Inhibition and oscillations in the human brain tissue in vitro.

  2019cites this paper
• Gap junctional communication between β‐ and δ‐cells: another player for suppression of glucagon release

  2018cites this paper
• LIGHTING A PATH TO PRECISION MEDICINE

  2018cites this paper
• Strategies for In Vivo Genome Editing in Nondividing Cells.

  2018cites this paper
• Modelling neurodegenerative diseases in vitro : Recent advances in 3D iPSC technologies

  2018cites this paper
• Optogenetics: Lighting a Path from the Laboratory to the Clinic

  2018cites this paper
• Free-floating adult human brain-derived slice cultures as a model to study the neuronal impact of Alzheimer's disease-associated Aβ oligomers.

  2018cites this paper
• Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery

  2018cites this paper
• A robust ex vivo experimental platform for molecular-genetic dissection of adult human neocortical cell types and circuits

  2018cites this paper
• Human Cerebrospinal fluid promotes long-term neuronal viability and network function in human neocortical organotypic brain slice cultures

  2017cites this paper
• Chronic ambulatory electrocorticography from human speech cortex

  2017cites this paper
• Applications of optogenetic and chemogenetic methods to seizure circuits: Where to go next?

  2017cites this paper
• Manufacturing Methods for Magnetic Resonance Microscopy Tools with Application to Neuroscience

  2017cites this paper
• Toward modeling the human nervous system in a dish: recent progress and outstanding challenges.

  2017cites this paper
• Exploring pain pathophysiology in patients

  2016cites this paper
• Edinburgh Research Explorer p-tau Ser356 is associated with Alzheimer's disease pathology and is lowered in brain slice cultures using the NUAK inhibitor WZ4003

  year unknowncites this paper