Effect of 50% enantiomeric excess bupivacaine mixture combined with pancuronium on neuromuscular transmission in rat phrenic nerve-diaphragm preparation; a pilot study

Background and Aims: Local anaesthetics are drugs that are widely used in clinical practice. However, the effects of these drugs on the neuromuscular junction and their influence on the blockade produced by non-depolarising neuromuscular blocking drugs are still under investigation. The aim of this study was to evaluate, in vitro, the influence of a 50% enantiomeric excess bupivacaine mixture on neuromuscular transmission and neuromuscular block produced by pancuronium. Methods: Rats were distributed into three groups (n = 5) according to the drug studied namely, 50% enantiomeric excess bupivacaine mixture (5 μg/mL); pancuronium (2 μg/mL); 50% enantiomeric excess bupivacaine mixture + pancuronium. The following parameters were evaluated: (1) Effects of a 50% enantiomeric excess bupivacaine mixture on membrane potential (MP) and miniature endplate potentials (MEPPs); (2) amplitude of diaphragmatic response before and 60 min after the addition of a 50% enantiomeric excess bupivacaine mixture; the degree of neuromuscular block with pancuronium and pancuronium combined with a 50% enantiomeric excess bupivacaine mixture. Results: A 50% enantiomeric excess bupivacaine mixture did not alter the amplitude of muscle response (MP) but decreased the frequency and amplitude of MEPP. The block produced by pancuronium was potentiated by a 50% enantiomeric excess bupivacaine mixture. Conclusion: A 50% enantiomeric excess bupivacaine mixture used alone did not affect neuromuscular transmission, but potentiated the neuromuscular block produced by pancuronium. No action was shown on the muscle fibre, and alterations on MEPPs demonstrated a presynaptic action.

• Publication year

  2015

• Venue

  Indian Journal of Anaesthesia

• Publication date

  2015-11-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.4103/0019-5049.170019PMID 26755834PMCID 4697241
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The RECITE Study: A Canadian Prospective, Multicenter Study of the Incidence and Severity of Residual Neuromuscular Blockade

  2015cited by this paper
• Use of neuromuscular blockers in Brazil.

  2015cited by this paper
• Effect of ropivacaine combined with pancuronium on neuromuscular transmission and effectiveness of neostigmine and 4-aminopyridine for blockade reversal: experimental study

  2015cited by this paper
• [Effect of ropivacaine combined with pancuronium on neuromuscular transmission and effectiveness of neostigmine and 4-aminopyridine for blockade reversal: experimental study].

  2015cited by this paper
• Effect of Epidural Levobupivacaine on Recovery from Vecuronium-Induced Neuromuscular Block in Patients Undergoing Lower Abdominal Surgery

  2011cited by this paper
• Influence of procainamide on the neuromuscular blockade caused by rocuronium and investigation on the mechanism of action of procainamide on the neuromuscular junction.

  2007cited by this paper
• [Comparison of the hemodynamic effects in acute intoxication with racemic bupivacaine and with 50% enantiomeric excess mixture (S75-R25): an experimental study in dogs.].

  2006cited by this paper
• [Use of neuromuscular blockers in Brazil].

  2004cited by this paper
• [Influence of epidural lidocaine injection on vecuronium-induced neuromuscular blockade].

  2004cited by this paper
• Neuromuscular blocking drugs and allergic risk.

  2003cited by this paper
• Membrane effects of ropivacaine compared with those of bupivacaine and mepivacaine

  2002cited by this paper
• Surface active drugs: self-association and interaction with membranes and surfactants. Physicochemical and biological aspects.

  2000cited by this paper
• A molecular basis for the different local anesthetic affinities of resting versus open and inactivated states of the sodium channel.

  1999cited by this paper
• Sodium channels as molecular targets for antiepileptic drugs.

  1998cited by this paper
• OBSERVATIONS ON THE ISOLATED PHRENIC NERVE DIAPHRAGM PREPARATION OF THE RAT

  1997cited by this paper
• Mechanism of Action of Local Anesthetics on Synaptic Transmission in the Rat

  1990cited by this paper
• Interactions of bupivacaine with ionic channels of the nicotinic receptor. Analysis of single-channel currents.

  1984cited by this paper
• Local anesthetics and histrionicotoxin are allosteric inhibitors of the acetylcholine receptor. Studies of clonal muscle cells.

  1982cited by this paper
• The kinetics of local anesthetic blockade of end-plate channels.

  1982cited by this paper
• Prejunctional and Postjunctional Cholinoceptors at the Neuromusclar Junction

  1980cited by this paper
• Local anaesthetics transiently block currents through single acetylcholine‐receptor channels.

  1978cited by this paper
• Interaction of muscle relaxants and local anesthetics at the neuromuscular junction.

  1978cited by this paper
• The effects of local anesthetics on motor nerve terminals.

  1968cited by this paper
• Alteration by Xylocaine (Lidocaine) and Its Derivatives of the Time Course of the End Plate Potential

  1968cited by this paper
• EFFECTS OF CENTRAL DEPRESSANT DRUGS UPON ACETYLCHOLINE RELEASE.

  1964cited by this paper
• Observations on the isolated phrenic nerve diaphragm preparation of the rat.

  1946cited by this paper

• Epidural administration of ropivacaine and its effects on the pharmacodynamics of rocuronium: Randomized controlled trial. Interaction between ropivacaine and rocuronium

  2020cites this paper
• Influence of different local anesthetics on atracurium neuromuscular blockade on rats☆

  2020cites this paper
• Influência de diferentes anestésicos locais no bloqueio neuromuscular produzido pelo atracúrio em ratos

  2020cites this paper
• Reversal of neuromuscular block produced by local anaesthetics combined with pancuronium. Experimental study

  2018cites this paper
• Association between levobupivacaine and pancuronium. Interference in neuromuscular transmission and blockade in rats.

  2016cites this paper