Cooperative modulation of voltage-dependent sodium channels by brevetoxin and classical neurotoxins in cultured bovine adrenal medullary cells.

The effects of Ptychodiscus brevis toxin (PbTx-3) on 22Na influx, 45Ca influx and catecholamine secretion were examined in cultured bovine adrenal medullary cells and compared with the effects of classical neurotoxins. PbTx-3 alone had no effects, but greatly enhanced veratridine (30 microM)-induced Na influx, Ca influx and secretion, with a EC50 of 30, 25 and 23 nM, respectively. PbTx-3 (1 microM) reduced EC50 values of veratridine approximately 3-fold and increased the maximal responses caused by saturating concentration (300 microM) of veratridine approximately 1.3 fold. alpha- and beta-Scorpion venom shifted the concentration-response curves of veratridine to the left without altering maximal responses. PbTx-3 in combination with either alpha- or beta-scorpion venom showed only additive effects on Na influx, but augmented veratridine (30 microM)-induced Na influx to a greater extent than PbTx-3, alpha- or beta-scorpion venom alone. Na influx due to these toxins was abolished by 1 microM saxitoxin. Our results suggest that Na channels in adrenal medullary cells have neurotoxin receptors for brevetoxin that allosterically stimulate Na influx initiated by veratridine, leading to increased Ca influx and catecholamine secretion. Allosteric interactions do not exist between brevetoxin and alpha-scorpion venom, or between brevetoxin and beta-scorpion venom, but once Na channels are gated by veratridine, these toxins cooperatively augment Na influx.

• Publication year

  1992

• Venue

  Journal of Pharmacology and Experimental Therapeutics

• Publication date

  1992-12-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0022-3565(25)10476-xPMID 1335063
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Modulation by ouabain and diphenylhydantoin of veratridine-induced 22Na influx and its relation to 45Ca influx and the secretion of catecholamines in cultured bovine adrenal medullary cells

  2004cited by this paper
• Sequence and genomic structure of the human adult skeletal muscle sodium channel alpha subunit gene on 17q.

  1992cited by this paper
• Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel.

  1992cited by this paper
• A Mutant of TTX-Resistant Cardiac Sodium Channels with TTX-Sensitive Properties

  1992cited by this paper
• Conotoxin GIIIA: selective inhibition of 22Na influx via voltage-dependent Na channels in adrenal medullary cells

  1990cited by this paper
• A neutral amino acid change in segment IIS4 dramatically alters the gating properties of the voltage-dependent sodium channel.

  1990cited by this paper
• Changes in sodium channel gating produced by point mutations in a cytoplasmic linker.

  1990cited by this paper
• Ion channels in nerve and muscle cells.

  1990cited by this paper
• Brevetoxins: unique polyether dinoflagellate toxins

  1989cited by this paper
• Localization of the receptor site for alpha-scorpion toxins by antibody mapping: implications for sodium channel topology.

  1989cited by this paper
• Molecular cloning of a putative tetrodotoxin-resistant rat heart Na+ channel isoform.

  1989cited by this paper
• A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II

  1989cited by this paper
• Primary structure and functional expression of a mammalian skeletal muscle sodium channel.

  1989cited by this paper
• A rat brain Na+ channel alpha subunit with novel gating properties.

  1988cited by this paper
• Ciguatoxin and brevetoxins share a common receptor site on the neuronal voltage‐dependent Na+ channel

  1987cited by this paper
• Allosteric modulation of neurotoxin binding to voltage-sensitive sodium channels by Ptychodiscus brevis toxin 2.

  1987cited by this paper
• Binding of [3H]saxitoxin to the voltage-dependent Na channels and inhibition of 22Na influx in bovine adrenal medullary cells.

  1987cited by this paper
• Activation of the Voltage‐Sensitive Sodium Channel by a β‐Scorpion Toxin in Rat Brain Nerve‐Ending Particles

  1986cited by this paper
• Brevetoxins, unique activators of voltage-sensitive sodium channels, bind to specific sites in rat brain synaptosomes.

  1986cited by this paper
• Nerve membrane sodium channels as the target site of brevetoxins at neuromuscular junctions

  1986cited by this paper
• Expression of functional sodium channels from cloned cDNA

  1986cited by this paper
• Discrimination of muscle and neuronal Na-channel subtypes by binding competition between [3H]saxitoxin and mu-conotoxins.

  1986cited by this paper
• Geographutoxin II, a novel peptide inhibitor of Na channels of skeletal muscles and autonomic nerves.

  1986cited by this paper
• Interaction of brevetoxin A with a new receptor site on the sodium channel.

  1985cited by this paper
• Influx of 22Na through acetylcholine receptor-associated Na channels: relationship between 22Na influx, 45Ca influx and secretion of catecholamines in cultured bovine adrenal medulla cells.

  1985cited by this paper
• Actions of Ptychodiscus brevis toxins on nerve and muscle membranes.

  1985cited by this paper
• The molecular basis of neuronal excitability.

  1984cited by this paper
• Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence

  1984cited by this paper
• Depolarizing action of a red-tide dinoflagellate brevetoxin on axonal membranes.

  1984cited by this paper
• Positive inotropic and toxic effects of brevetoxin-B on rat and guinea pig heart.

  1984cited by this paper
• Actions of Ptychodiscus brevis Red Tide Toxin on Metabolic and Transmitter‐Releasing Properties of Synaptosomes

  1982cited by this paper
• Toxin T4(6) from Ptychodiscus brevis (formerly Gymnodinium breve) enhances activation of voltage-sensitive sodium channels by veratridine.

  1981cited by this paper
• Concept of paraneurons.

  1977cited by this paper
• The chemical estimation of adrenaline-like substances in blood.

  1952cited by this paper

• An integrated systems-level model of the toxicity of brevetoxin based on high-resolution magic-angle spinning nuclear magnetic resonance (HRMAS NMR) metabolic profiling of zebrafish embryos.

  2021cites this paper
• Up-Regulation of NaV1.7 Sodium Channels Expression by Tumor Necrosis Factor-&agr; in Cultured Bovine Adrenal Chromaffin Cells and Rat Dorsal Root Ganglion Neurons

  2014cites this paper
• Dexmedetomidine and clonidine inhibit the function of NaV1.7 independent of α2-adrenoceptor in adrenal chromaffin cells

  2011cites this paper
• Brevenal Inhibits Pacific Ciguatoxin-1B-Induced Neurosecretion from Bovine Chromaffin Cells

  2008cites this paper
• Voltage-dependent Na(v)1.7 sodium channels: multiple roles in adrenal chromaffin cells and peripheral nervous system.

  2008cites this paper
• Effects of Sodium Channel-Targeted Conotoxins on Catecholamine Release in Adrenal Chromaffin Cells

  2008cites this paper
• Lysophosphatidic acid‐LPA1 receptor–Rho–Rho kinase‐induced up‐regulation of Nav1.7 sodium channel mRNA and protein in adrenal chromaffin cells: enhancement of 22Na+ influx, 45Ca2+ influx and catecholamine secretion

  2008cites this paper
• Lithium inhibits function of voltage-dependent sodium channels and catecholamine secretion independent of glycogen synthase kinase-3 in adrenal chromaffin cells.

  2007cites this paper
• Voltage‐dependent Nav1.7 sodium channels: multiple roles in adrenal chromaffin cells and peripheral nervous system

  2007cites this paper
• Minimal sodium channel pore consisting of S5-P-S6 segments preserves intracellular pharmacology.

  2005cites this paper
• STABILITY AND POSTTRANSCRIPTIONAL CONTROL IN ADRENAL CHROMAFFIN CELLS

  2004cites this paper
• Regulation of cell surface expression of voltage-dependent Nav1.7 sodium channels: mRNA stability and posttranscriptional control in adrenal chromaffin cells.

  2004cites this paper
• Destabilization of Nav1.7 Sodium Channel α-Subunit mRNA by Constitutive Phosphorylation of Extracellular Signal-Regulated Kinase: Negative Regulation of Steady-State Level of Cell Surface Functional Sodium Channels in Adrenal Chromaffin Cells

  2003cites this paper
• Destabilization of Na(v)1.7 sodium channel alpha-subunit mRNA by constitutive phosphorylation of extracellular signal-regulated kinase: negative regulation of steady-state level of cell surface functional sodium channels in adrenal chromaffin cells.

  2003cites this paper
• Regulation of Voltage‐Dependent Sodium Channel Expression in Adrenal Chromaffin Cells

  2002cites this paper
• A “Minimal” Sodium Channel Construct Consisting of Ligated S5-P-S6 Segments Forms a Toxin-activatable Ionophore*

  2002cites this paper
• Differential effects of short and prolonged exposure to carvedilol on voltage-dependent Na(+) channels in cultured bovine adrenal medullary cells.

  2002cites this paper
• Up-regulation of cell surface sodium channels by cyclosporin A, FK506, and rapamycin in adrenal chromaffin cells.

  2001cites this paper
• Heterogeneous increases of cytoplasmic calcium: distinct effects on down‐regulation of cell surface sodium channels and sodium channel subunit mRNA levels

  2001cites this paper
• Protein kinase C-alpha and -epsilon down-regulate cell surface sodium channels via differential mechanisms in adrenal chromaffin cells.

  2000cites this paper
• Protein Kinase C‐α and ‐ε Down‐Regulate Cell Surface Sodium Channels via Differential Mechanisms in Adrenal Chromaffin Cells

  2000cites this paper
• Structure, function and pharmacology of voltage-gated sodium channels

  2000cites this paper
• Short‐ and long‐term differential effects of neuroprotective drug NS‐7 on voltage‐dependent sodium channels in adrenal chromaffin cells

  2000cites this paper
• Protein Kinase C and the Opposite Regulation of Sodium Channel α‐ and β1‐Subunit mRNA Levels in Adrenal Chromaffin Cells

  1999cites this paper
• Protein kinase C and the opposite regulation of sodium channel alpha- and beta1-subunit mRNA levels in adrenal chromaffin cells.

  1999cites this paper
• Effects of veratridine on sodium currents and fluxes.

  1998cites this paper
• Selective inhibition by riluzole of voltage-dependent sodium channels and catecholamine secretion in adrenal chromaffin cells

  1998cites this paper
• Up‐Regulation of Sodium Channel Subunit mRNAs and Their Cell Surface Expression by Antiepileptic Valproic Acid: Activation of Calcium Channel and Catecholamine Secretion in Adrenal Chromaffin Cells

  1997influential citation
• Protein Kinase C‐Mediated Down‐Regulation of Voltage‐Dependent Sodium Channels in Adrenal Chromaffin Cells

  1996cites this paper
• Up‐Regulation of Functional Voltage‐Dependent Sodium Channels by Insulin in Cultured Bovine Adrenal Chromaffin Cells

  1996cites this paper
• Up-regulation of functional voltage-dependent sodium channels by cyclic AMP-dependent protein kinase in adrenal medulla.

  1996cites this paper
• Proadrenomedullin N‐Terminal 20 Peptide (PAMP), an Endogenous Anticholinergic Peptide: Its Exocytotic Secretion and Inhibition of Catecholamine Secretion in Adrenal Medulla

  1995cites this paper
• Characterization of [3H]brevetoxin binding to voltage-dependent sodium channels in adrenal medullary cells

  1994cites this paper
• Ionic factors affecting the association of tyrosine hydroxylase with chromaffin granules in the adrenal medullary cell.

  1994cites this paper