Study of rumen metagenome community using qPCR under different diets

The aim of this study was to detect the major bacteria present in rumen microbiota. Here, we performed qPCR based absolute quantitation of selected rumen microbes in rumen fluid of river buffalo adapted to varying proportion of concentrate to roughage diets. Animals were adapted to roughage-to-concentrate ratio in the proportion of 100:00 (T1), 75:25 (T2), 50:50 (T3) and 25:75 (T4) respectively for 30 days. At the end of each treatment, rumen fluid was collected at 0 h and 2 h after feeding. It was found that among fibrolytic bacteria Ruminococcus flavefaciens (2.22 × 108 copies/ml) were highest in T2 group and followed by 1.11 × 108 copies/ml for Fibrobacter succinogenes (T2), 2.56 × 107 copies/ml for Prevotella ruminicola (T1) and 1.25 × 107 copies/ml for Ruminococcus albus (T4). In non-fibrolytic bacteria, the Selenomonas ruminantium (2.62 × 107 copies/ml) was predominant in group T3 and followed by Treponema bryantii (2.52 × 107copies/ml) in group T1, Ruminobacter amylophilus (1.31 × 107copies/ml) in group T1 and Anaerovibrio lipolytica (2.58 × 106 copies/ml) in group T4. It is most notable that R. flavefaciens were the highest in population in the rumen of Surti buffalo fed wheat straw as roughage source.

• Publication year

  2014

• Venue

  Meta Gene

• Publication date

  2014-02-19

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology, Environmental Science

• Identifiers
  DOI 10.1016/j.mgene.2014.01.001PMID 25606402PMCID 4287863
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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