Immunological identification of 1,25-dihydroxyvitamin D3 receptors in human promyelocytic leukemic cells (HL-60) during homologous regulation.

Immunological techniques were utilized to detect 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) receptor levels and to characterize physical/chemical changes in receptors in human promyelocytic leukemic cells (HL-60) during continuous exposure to hormone. The monoclonal antibody (IVG8C11) raised against the porcine intestinal 1,25-(OH)2D3 receptor immunoprecipitated quantitatively 1,25-(OH)2D3 receptors in nuclear extracts from HL-60 cells. The highly enriched immunoprecipitated receptors were electrophoresed on sodium dodecyl sulfate-polyacrylamide gels and transferred to polyvinylidene difluoride membranes, which were probed with 125I-labeled IVG8C11. The basal receptor from the cells treated with 1,25-(OH)2D3 for 2 h was detected as a single form at 53 kDa. Moreover, receptors were shown to be up-regulated at 12 h and down-regulated at 48 and 72 h in the continuous presence of hormone as evidenced by the ratio of density of the bands, 1.0 (2 h):4.2 (12 h):1.2 (48 h):0.9 (72 h), as measured by laser scanning densitometry. The up- and down-regulated receptors were also detected as single forms and had the same molecular mass as the basal receptor. Therefore, the data presented here strongly support the hypothesis of homologous regulation of 1,25-(OH)2D3 receptors in intact human target cells.

• Publication year

  1989

• Venue

  Journal of Biological Chemistry

• Publication date

  1989-08-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)80055-2PMID 2547772
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• 1,25-Dihydroxyvitamin D3 and the regulation of macrophage function

  2006cited by this paper
• Size and charge of the functional 1,25-dihydroxyvitamin D receptor in porcine intestine.

  1987cited by this paper
• Induction of monocytic differentiation by calcitriol (1,25-dihydroxyvitamin D3) in the human promyelocytic leukemic cell line (HL-60) in serum-free medium.

  1987cited by this paper
• Biological activity of fluorinated vitamin D analogs at C-26 and C-27 on human promyelocytic leukemia cells, HL-60.

  1987cited by this paper
• 1,25-Dihydroxyvitamin D3 regulation of phorbol ester receptors in HL-60 leukemia cells.

  1987cited by this paper
• Induction of monocytic differentiation of HL-60 cells by 1,25-dihydroxyvitamin D analogs.

  1987cited by this paper
• Avian and mammalian receptors for 1,25-dihydroxyvitamin D3: in vitro translation to characterize size and hormone-dependent regulation.

  1987cited by this paper
• Ligand-dependent regulation of the 1,25-dihydroxyvitamin D3 receptor in rat osteosarcoma cells.

  1987cited by this paper
• Homologous up-regulation of the 1,25 (OH)2 vitamin D3 receptor in rats.

  1986cited by this paper
• 1,25-Dihydroxyvitamin D3 promotes in vitro morphologic and enzymatic changes in normal human monocytes consistent with their differentiation into macrophages.

  1986cited by this paper
• Alteration in insulin receptor expression accompanying differentiation of HL-60 leukemia cells.

  1986cited by this paper
• Hormonal responses to 1,25‐dihydroxyvitamin D3 in cultured mouse osteoblast‐like cells—modulation by changes in receptor level

  1986cited by this paper
• Monoclonal antibodies to the porcine intestinal receptor for 1,25-dihydroxyvitamin D3: interaction with distinct receptor domains.

  1986cited by this paper
• Effect of in vitro differentiation on phorbol diester receptor number in human promyelocytic leukemia (HL-60) cells.

  1986cited by this paper
• Vitamin D resistance and alopecia: a kindred with normal 1,25-dihydroxyvitamin D binding, but decreased receptor affinity for deoxyribonucleic acid.

  1985cited by this paper
• Identification of the porcine intestinal 1,25-dihydroxyvitamin D3 receptor on sodium dodecyl sulfate/polyacrylamide gels by renaturation and immunoblotting.

  1985cited by this paper
• Regulation of 1,25-dihydroxyvitamin D3 receptors by vitamin D analogs in cultured mammalian cells.

  1985cited by this paper
• Regulation of the 1,25-dihydroxyvitamin D3 receptor by 1,25-dihydroxyvitamin D3 in intact human cancer cells.

  1985cited by this paper
• Influence of 1,25-dihydroxyvitamin D3 on cultured osteogenic sarcoma cells: correlation with the 1,25-dihydroxyvitamin D3 receptor.

  1984cited by this paper
• 1,25-Dihydroxyvitamin D3-induced differentiation in a human promyelocytic leukemia cell line (HL-60): receptor-mediated maturation to macrophage-like cells

  1984cited by this paper
• Induction of a high phagocytic capability in P388D1, a macrophage-like tumor cell line, by 1 alpha, 25-dihydroxyvitamin D3.

  1984cited by this paper
• Regulation of 1,25(OH)2 vitamin D3 receptor content in cultured LLC-PK1 kidney cells limits hormonal responsiveness.

  1983cited by this paper
• 1,25-dihydroxyvitamin D3 receptors in human leukocytes.

  1983cited by this paper
• Functional defect of variant clones of a human myeloid leukemia cell line (HL-60) resistant to 1 alpha,25-dihydroxyvitamin D3.

  1983cited by this paper
• Vitamin D metabolites change the phenotype of monoblastic U937 cells.

  1983cited by this paper
• 1,25-dihydroxyvitamin D3 inhibits proliferation of human promyelocytic leukaemia (HL60) cells and induces monocyte-macrophage differentiation in HL60 and normal human bone marrow cells.

  1983cited by this paper
• Cooperative effect of 1 alpha,25-dihydroxyvitamin D3 and dexamethasone in inducing differentiation of mouse myeloid leukemia cells.

  1983cited by this paper
• Vitamin D: recent advances.

  1983cited by this paper
• Specific high-affinity receptors for 1,25-dihydroxyvitamin D3 in human peripheral blood mononuclear cells: presence in monocytes and induction in T lymphocytes following activation.

  1983cited by this paper
• 1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60).

  1982cited by this paper
• Differentiation of mouse myeloid leukemia cells induced by 1 alpha,25-dihydroxyvitamin D3.

  1981cited by this paper
• Biochemical properties of 1 alpha, 25-dihydroxyvitamin D receptors.

  1980cited by this paper
• An hydroxylapatite batch assay for the quantitation of 1alpha,25-dihydroxyvitamin D3-receptor complexes.

  1979cited by this paper
• Estrogen control of progesterone receptor in human breast cancer. Correlation with nuclear processing of estrogen receptor.

  1978cited by this paper
• Nuclear mechanisms of estrogen action. Effects of estradiol and anti-estrogens on estrogen receptors and nuclear receptor processing.

  1978cited by this paper
• Production and properties of vitamin-D-induced mRNA for chick calcium-binding protein.

  1976cited by this paper
• 1 Alpha,25-dihydroxycholecalciferol receptors in intestine. I. Association of 1 alpha,25-dihydroxycholecalciferol with intestinal mucosa chromatin.

  1974cited by this paper
• The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.

  1973cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• Vitamin D gene regulation

  2020cites this paper
• Inhibitors for the Vitamin D Receptor-Coregulator Interaction.

  2016cites this paper
• Vitamin D3 receptor is highly expressed in Hodgkin’s lymphoma

  2012cites this paper
• Vitamin D 3 receptor is highly expressed in Hodgkin ’ s lymphoma

  2012cites this paper
• Vitamin D and Hematologic Malignancies

  2011cites this paper
• human monocytes into macrophages expression are developmentally regulated during differentiation of 1,25-dihydroxyvitamin D3 production and vitamin D3 receptor

  2011cites this paper
• Chapter 88 – Hematological Malignancy

  2011cites this paper
• Regulation of VDR expression in rat and human intestine and liver--consequences for CYP3A expression.

  2010cites this paper
• Regulation of metabolizing enzymes and transporters for drugs and bile salts in human and rat intestine and liver: a study with precision-cut slices

  2009cites this paper
• Regulation of metabolizing enzymes and transporters for drugs and bile salts in human and rat intestine and liver

  2009cites this paper
• 1alpha,25-Dihydroxyvitamin D(3)-26,23-lactam analogues function as vitamin D receptor antagonists in human and rodent cells.

  2008cites this paper
• Synergistic Enhancement by 1 2O-Tetradecanoylphorbol-1 3-acetate and Dibutyryl cAMP of la , 25-Dihydroxyvitamin D 3 Action in Human Promyelocytic Leukemic HL-60 Cells

  2006cites this paper
• CHAPTER 12 – Vitamin D Receptor Promoter and Regulation of Receptor Expression

  2005cites this paper
• The antagonism between 2-methyl-1,25-dihydroxyvitamin D3 and 2-methyl-20-epi-1,25-dihydroxyvitamin D3 in non-genomic pathway-mediated biological responses induced by 1alpha,25-dihydroxyvitamin D3 assessed by NB4 cell differentiation.

  2005cites this paper
• 1 a , 25-Dihydroxyvitamin D 3-induced Regulation of Protein Kinase C Gene Expression during HL-60 Cell Differentiation 1

  2005cites this paper
• CHAPTER 96 – Vitamin D and Hematological Malignancy

  2005cites this paper
• Lipopolysaccharide negatively modulates vitamin D action by down-regulating expression of vitamin D-induced VDR in human monocytic THP-1 cells.

  2004cites this paper
• Mechanisms involved in the induced differentiation of leukemia cells.

  2003cites this paper
• Modulation by cAMP of 1alpha,25-dihydroxyvitamin D3 sensitivity of murine erythroleukemia cells.

  2001cites this paper
• The role of heat shock protein 70 in vitamin D receptor function.

  2001cites this paper
• Modulation by cAMP of 1α,25-Dihydroxyvitamin D3 Sensitivity of Murine Erythroleukemia Cells

  2001cites this paper
• Stimulation by 1,25-dihydroxyvitamin D3 of insulin receptor expression and insulin responsiveness for glucose transport in U-937 human promonocytic cells.

  2000cites this paper
• 1α,25‐Dihydroxyvitamin D3‐26,23‐lactone analogs antagonize differentiation of human leukemia cells (HL‐60 cells) but not of human acute promyelocytic leukemia cells (NB4 cells)

  1999cites this paper
• Antagonistic Action of Novel 1α,25-Dihydroxyvitamin D3-26,23-lactone Analogs on Differentiation of Human Leukemia Cells (HL-60) Induced by 1α,25-Dihydroxyvitamin D3 *

  1999cites this paper
• Leukemia cell differentiation: cellular and molecular interactions of retinoids and vitamin D.

  1999cites this paper
• Synergistic enhancement by 12-O-tetradecanoylphorbol-13-acetate and dibutyryl cAMP of 1alpha,25-dihydroxyvitamin D3 action in human promyelocytic leukemic HL-60 cells.

  1999cites this paper
• Inhibition by 1alpha,25-dihydroxyvitamin D3 of activin A-induced differentiation of murine erythroleukemic F5-5 cells.

  1997cites this paper
• Genomic mechanisms involved in the pleiotropic actions of 1,25-dihydroxyvitamin D3.

  1996cites this paper
• 1,25-dihydroxyvitamin D3 and 22-oxacalcitriol prevent the decrease in vitamin D receptor content in the parathyroid glands of uremic rats.

  1996cites this paper
• Biological activities of 26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3 on human promyelocytic leukemic HL-60 cells: effects of fetal bovine serum and of incubation time.

  1995cites this paper
• Mode of differentiation of human promyelocytic leukemia cell line, HL-60, by 1 alpha,25-dihydroxyvitamin D3.

  1995cites this paper
• Influence of high glucose on 1,25‐dihydroxyvitamin D3‐induced effect on human osteoblast‐like MG‐63 cells

  1995cites this paper
• The roles of calcium and 1,25-dihydroxyvitamin D3 in the regulation of vitamin D receptor expression by rat parathyroid glands.

  1995cites this paper
• Monocytic Differentiation of Acute Promyelocytic Leukemia Cells in Response to 1,25-Dihydroxyvitamin D3 Is Independent of Nuclear Receptor Binding (*)

  1995cites this paper
• Modulation of vitamin D receptor and estrogen receptor by 1,25(OH)2-vitamin D3 in T-47D human breast cancer cells.

  1995cites this paper
• Effects of vitamin D, dietary calcium and vitamin D restriction, pregnancy and lactation on gene expression of calcium transporting factors

  1995cites this paper
• Effects of 1,25-dihydroxyvitamin D3 on proliferation and differentiation of Caco-2 cells.

  1994cites this paper
• Impaired homologous upregulation of vitamin D receptor in rats with chronic renal failure.

  1994cites this paper
• 1,25-dihydroxyvitamin D3 regulates the synthesis of nerve growth factor in primary cultures of glial cells.

  1994cites this paper
• Presence of gene expression of vitamin D receptor and 24‐hydroxylase in OK cells

  1994cites this paper
• The molecular basis of secondary hyperparathyroidism in chronic renal failure.

  1994cites this paper
• Steroid hormone modulation of vitamin D receptor levels in human MG-63 osteosarcoma cells.

  1994cites this paper
• 1,25-dihydroxyvitamin D3 enhances the transcription and expression of the inositol trisphosphate receptor gene in HL-60 cells.

  1993cites this paper
• Regulation of release of hepatocyte growth factor from human promyelocytic leukemia cells, HL-60, by 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol 13-acetate, and dibutyryl cyclic adenosine monophosphate.

  1993cites this paper
• The D domain of the thyroid hormone receptor alpha 1 specifies positive and negative transcriptional regulation functions.

  1993cites this paper
• Stabilization of the vitamin D receptor in rat osteosarcoma cells through the action of 1,25-dihydroxyvitamin D3.

  1993cites this paper
• 1,25-dihydroxyvitamin D3 production and vitamin D3 receptor expression are developmentally regulated during differentiation of human monocytes into macrophages.

  1993cites this paper
• Sex steroids induced up-regulation of 1,25-(OH)2 vitamin D3 receptors in T 47D breast cancer cells.

  1993cites this paper
• The necessity for calcium for increased renal vitamin D receptor in response to 1,25-dihydroxyvitamin D.

  1993cites this paper
• Effect of substituting fluorine for hydrogen at C-26 and C-27 on the side chain of 1,25-dihydroxyvitamin D3.

  1993cites this paper
• Vitamin D3 binding activity during leukemic cell differentiation.

  1993cites this paper
• Effect of uremic serum on 1,25-dihydroxyvitamin D3-induced differentiation of human promyelocytic leukemia cell, HL-60.

  1993cites this paper
• 1,25(OH)2-vitamin D3 mediated changes in mRNA for c-myc and 1,25(OH)2D3 receptor in HL-60 cells and related subclones.

  1993cites this paper
• Tissue-specific regulation of 1,25-dihydroxyvitamin D action in the target tissues of rats

  1993cites this paper
• The effect of 1,25-dihydroxyvitamin D3 on lymphoma cell lines and expression of vitamin D receptor in lymphoma.

  1993cites this paper
• Resistance to 1,25-dihydroxyvitamin D3 of a deoxycytidine kinase-deficient variant of human leukemia HL60 cells.

  1992cites this paper
• A new, highly sensitive assay for 1,25-dihydroxyvitamin D not requiring high-performance liquid chromatography: application of monoclonal antibody against vitamin D receptor to radioreceptor assay.

  1992cites this paper
• Short-term regulation of the renal vitamin D receptor in rats by 1,25-dihydroxycholecalciferol is calcium insensitive.

  1992cites this paper
• A single receptor identical with that from intestine/T47D cells mediates the action of 1,25-dihydroxyvitamin D-3 in HL-60 cells.

  1992cites this paper
• Mechanism of action of 1,25-dihydroxyvitamin D on target gene expression.

  1992cites this paper
• Up-regulation of the vitamin D receptor in response to 1,25-dihydroxyvitamin D3 results from ligand-induced stabilization.

  1992cites this paper
• Dibutyryl cAMP enhances the effect of 1,25-dihydroxyvitamin D3 on a human promyelocytic leukemia cell, HL-60, at both the receptor and the postreceptor steps.

  1992cites this paper
• Effects of uremic serum on 1,25-dihydroxyvitamin D3-induced differentiation of a human promyelocytic leukemia cell line, HL-60.

  1991cites this paper
• 1,25-Dihydroxyvitamin D3 receptor RNA: expression in hematopoietic cells.

  1991cites this paper
• Homologous and heterologous regulation of 1,25-dihydroxyvitamin D-3 receptor mRNA levels in human osteosarcoma cells.

  1991cites this paper
• Cholesterol and lipoprotein metabolism of human promyelocytic leukemic HL-60 induced macrophages.

  1991cites this paper
• Recent advances in the molecular mechanism of action of 1, 25-dihydroxyvitamin D3

  1991cites this paper
• Differentiation‐related regulation of 1,25 dihydroxy vitamin D3 receptor mRNA in human leukaemia cells HL‐60

  1991cites this paper
• Effect of intermittent oral 1,25(OH)2D3 therapy on bone Gla protein in dialysis patients.

  1991cites this paper
• 1 alpha,25-dihydroxyvitamin D3-induced regulation of protein kinase C gene expression during HL-60 cell differentiation.

  1991cites this paper
• Vitamin D3 receptors: structure and function in transcription.

  1991cites this paper
• Short-term effects of physiological and pharmacological doses of estradiol on estrogen receptor and uterine growth.

  1991cites this paper
• Regulation of 1,25-dihydroxyvitamin D3 receptor gene expression by 1,25-dihydroxyvitamin D3 in the parathyroid in vivo.

  1990cites this paper
• Mechanism of action of 1,25-dihydroxyvitamin D3: 1990 version

  1990cites this paper
• 1,25-Dihydroxyvitamin D3 up-regulates the 1,25-dihydroxyvitamin D3 receptor in vivo.

  1989cites this paper