Comparative analysis of volatile metabolomics signals from melanoma and benign skin: a pilot study

The analysis of volatile organic compounds (VOC) as biomarkers of cancer is both promising and challenging. In this pilot study, we used an untargeted approach to compare volatile metabolomic signatures of melanoma and matched control non-neoplastic skin from the same patient. VOC from fresh (non-fixed) biopsied tissue were collected using the headspace solid phase micro extraction method (HS SPME) and analyzed by gas chromatography and mass spectrometry (GCMS). We applied the XCMS analysis platform and MetaboAnalyst software to reveal many differentially expressed metabolic features. Our analysis revealed increased levels of lauric acid (C12:0) and palmitic acid (C16:0) in melanoma. The identity of these compounds was confirmed by comparison with chemical standards. Increased levels of these fatty acids are likely to be a consequence of up-regulated de novo lipid synthesis, a known characteristic of cancer. Increased oxidative stress is likely to cause an additional increase in lauric acid. Implementation of this study design on larger number of cases will be necessary for the future metabolomics biomarker discovery applications.

• Publication year

  2013

• Venue

  Metabolomics

• Publication date

  2013-03-30

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1007/s11306-013-0523-zPMID 24039618PMCID 3769583
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Absence of distinguishing senescence traits in human melanocytic nevi.

  2012cited by this paper
• CAMERA: an integrated strategy for compound spectra extraction and annotation of liquid chromatography/mass spectrometry data sets.

  2012cited by this paper
• XCMS Online: a web-based platform to process untargeted metabolomic data.

  2012cited by this paper
• SEER Cancer Statistics Review, 1975–2009 (Vintage 2009 Populations)

  2012influential reference
• A case report - Volatile metabolomic signature of malignant melanoma using matching skin as a control.

  2011cited by this paper
• A quantitative proteomic analysis of FFPE melanoma

  2011cited by this paper
• Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst

  2011cited by this paper
• Determination of volatile organic compounds as biomarkers of lung cancer by SPME-GC-TOF/MS and chemometrics.

  2011cited by this paper
• Investigation of urinary volatile organic metabolites as potential cancer biomarkers by solid-phase microextraction in combination with gas chromatography-mass spectrometry

  2011cited by this paper
• Colorectal cancer screening with odour material by canine scent detection

  2011cited by this paper
• High-temperature gas chromatography-mass spectrometry for skin surface lipids profiling[S]

  2011cited by this paper
• Urinary Volatile Compounds as Biomarkers for Lung Cancer: A Proof of Principle Study Using Odor Signatures in Mouse Models of Lung Cancer

  2010cited by this paper
• Quantitative determination of some volatile suspected allergens in cosmetic creams spread on skin by direct contact sorptive tape extraction-gas chromatography-mass spectrometry.

  2010cited by this paper
• Differential Volatile Signatures from Skin, Naevi and Melanoma: A Novel Approach to Detect a Pathological Process

  2010cited by this paper
• Selection of tumorigenic melanoma cells using ALDH.

  2010cited by this paper
• Volatile biomarkers in the breath of women with breast cancer

  2010cited by this paper
• Detection of lung, breast, colorectal, and prostate cancers from exhaled breath using a single array of nanosensors

  2010cited by this paper
• Myeloid Cells in the Tumor Microenvironment: Modulation of Tumor Angiogenesis and Tumor Inflammation

  2010cited by this paper
• MetaboAnalyst: a web server for metabolomic data analysis and interpretation

  2009cited by this paper
• Melanoma epidemiology.

  2009cited by this paper
• Non-P 450 aldehyde oxidizing enzymes : the aldehyde dehydrogenase superfamily

  2009cited by this paper
• Surgical Management of Melanoma-In-Situ Using a Staged Marginal and Central Excision Technique

  2009cited by this paper
• Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression

  2009cited by this paper
• A retention index calculator simplifies identification of plant volatile organic compounds.

  2009cited by this paper
• The global burden of cancer: priorities for prevention

  2009influential reference
• The biology of cancer: metabolic reprogramming fuels cell growth and proliferation.

  2008cited by this paper
• Analyses of volatile organic compounds from human skin

  2008influential reference
• Identification of melanoma with a gas sensor array

  2008cited by this paper
• Metabolome Analysis of Biosynthetic Mutants Reveals a Diversity of Metabolic Changes and Allows Identification of a Large Number of New Compounds in Arabidopsis1[W][OA]

  2008cited by this paper
• Human Ovarian Carcinomas Detected by Specific Odor

  2008cited by this paper
• Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase superfamily

  2008cited by this paper
• Editorial [Hot Topic: High Throughput In Vitro ADME/Tox Profiling for Drug Discovery(Guest Editor: Edward H. Kerns)]

  2008cited by this paper
• A study of the volatile organic compounds exhaled by lung cancer cells in vitro for breath diagnosis

  2007cited by this paper
• Proposed minimum reporting standards for chemical analysis

  2007cited by this paper
• A Reliable Biomarker Derived from Plasmalogens to Evaluate Malignancy and Metastatic Capacity of Human Cancers

  2007cited by this paper
• Sorptive tape extraction in the analysis of the volatile fraction emitted from biological solid matrices.

  2007cited by this paper
• Sexual hormones in human skin.

  2007cited by this paper
• Preliminary study of volatile organic compounds from breath and stomach tissue by means of solid phase microextraction and gas chromatography–mass spectrometry

  2007cited by this paper
• SEER Cancer Statistics Review, 1975-2003

  2006influential reference
• XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification.

  2006influential reference
• Diagnostic Accuracy of Canine Scent Detection in Early- and Late-Stage Lung and Breast Cancers

  2006cited by this paper
• The role of mass spectrometry in biomarker discovery and measurement.

  2006cited by this paper
• Fatty acid synthase expression in cutaneous melanocytic neoplasms

  2005cited by this paper
• Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples.

  2005cited by this paper
• BRAFE600-associated senescence-like cell cycle arrest of human naevi

  2005cited by this paper
• Olfactory detection of human bladder cancer by dogs: Cause or association?

  2004cited by this paper
• Olfactory detection of human bladder cancer by dogs: proof of principle study

  2004cited by this paper
• Fatty acid synthase expression in melanoma

  2003cited by this paper
• The transformation rate of moles (melanocytic nevi) into cutaneous melanoma: a population-based estimate.

  2003cited by this paper
• Volatile Markers of Breast Cancer in the Breath

  2003cited by this paper
• 4-Hydroxy-2-nonenal, a specific lipid peroxidation product, is elevated in lungs of patients with chronic obstructive pulmonary disease.

  2002cited by this paper
• Another sniffer dog for the clinic?

  2001cited by this paper
• Analysis of human skin emanations by gas chromatography/mass spectrometry. 2. Identification of volatile compounds that are candidate attractants for the yellow fever mosquito (Aedes aegypti).

  2000cited by this paper
• Role of aldehyde dehydrogenases in endogenous and xenobiotic metabolism.

  2000cited by this paper
• Volatile organic compounds in breath as markers of lung cancer: a cross-sectional study.

  1999cited by this paper
• Analysis of human skin emanations by gas chromatography/mass spectrometry. 1. Thermal desorption of attractants for the yellow fever mosquito (Aedes aegypti) from handled glass beads.

  1999cited by this paper
• Lactose absorption in patients with ovarian cancer.

  1999cited by this paper
• The global burden of cancer.

  1998cited by this paper
• Method for the collection and assay of volatile organic compounds in breath.

  1997cited by this paper
• Melanoma epidemiology.

  1997cited by this paper
• Loss of expression of the p16/cyclin-dependent kinase inhibitor 2 tumor suppressor gene in melanocytic lesions correlates with invasive stage of tumor progression.

  1995cited by this paper
• Aldehyde dehydrogenases and their role in carcinogenesis.

  1992influential reference
• DO ALL MELANOMAS COME FROM “MOLES”? A STUDY OF THE HISTOLOGICAL ASSOCIATION BETWEEN MELANOCYTIC NAEVI AND MELANOMA

  1990cited by this paper
• Sniffer dogs in the melanoma clinic?

  1989cited by this paper
• Ether lipids : biochemical and biomedical aspects

  1983cited by this paper

• Potential biomarkers of skin cancer diagnosis revealed through volatile metabolomics – A prospective research study

  2025cites this paper
• Lauric Acid Promotes Esophageal Squamous Cell Carcinoma via GPR84: Lipid Metabolic Dysregulation as a Therapeutic Target

  2025cites this paper
• Unveiling the Metabolomic Profile of Oily Sensitive Skin: A Non-Invasive Approach

  2024cites this paper
• Studying microbially induced corrosion on glass using ToF-SIMS.

  2024cites this paper
• The human volatilome meets cancer diagnostics: past, present, and future of noninvasive applications

  2024cites this paper
• Identification of a colony- and dose-dependent worker aggregation pheromone in the subterranean termite Reticulitermes virginicus

  2024cites this paper
• Clinical applications of volatilomic assays

  2024cites this paper
• Volatile organic compound detection of Buruli ulcer disease: Headspace analysis of Mycobacterium ulcerans and used gauzes of Buruli-compatible ulcers

  2024cites this paper
• Body Volatilome Study Strategy for COVID-19 Biomarker Identification Considering Exogenous Parameters

  2024cites this paper
• Analytical methods for the analysis of volatile natural products.

  2023cites this paper
• Relationship between serum amino acid levels and acne severity

  2023cites this paper
• An engineered culture vessel and flow system to improve the in vitro analysis of volatile organic compounds

  2023cites this paper
• Rapid Detection of Volatile Organic Metabolites in Urine by High-Pressure Photoionization Mass Spectrometry for Breast Cancer Screening: A Pilot Study

  2023cites this paper
• Sensitivity and specificity of in vivo COVID-19 screening by detection dogs: Results of the C19-Screendog multicenter study

  2023cites this paper
• Skin Cancer Metabolic Profile Assessed by Different Analytical Platforms

  2023influential citation
• Protective Effects of Naringenin from Citrus sinensis (var. Valencia) Peels against CCl4-Induced Hepatic and Renal Injuries in Rats Assessed by Metabolomics, Histological and Biochemical Analyses

  2022cites this paper
• Ultra‐Fast Portable and Wearable Sensing Design for Continuous and Wide‐Spectrum Molecular Analysis and Diagnostics

  2022cites this paper
• Nontargeted metabolomics to characterize the effects of isotretinoin on skin metabolism in rabbit with acne

  2022cites this paper
• Current Knowledge in Skin Metabolomics: Updates from Literature Review

  2022cites this paper
• An Overview on the Application of Solid Phase Microextraction in the Analysis of Volatile Organic Compounds as Potential Biomarkers of Cancer

  2022cites this paper
• Volatolomics in healthcare and its advanced detection technology

  2022cites this paper
• The study of distribution of ingested terbinafine on skin with ambient ionization tandem mass spectrometry

  2022cites this paper
• Skin bioelectronics towards long-term, continuous health monitoring.

  2022cites this paper
• 1H NMR-based metabolomics of skin squamous cell carcinoma and peri-tumoral region tissues.

  2022cites this paper
• Investigation of the relationship between skin-emitted volatile fatty acids and skin surface acidity in healthy participants—a pilot study

  2021cites this paper
• Application of omics technologies in dermatological research and skin management

  2021cites this paper
• Metabolomic profile of cancer stem cell‐derived exosomes from patients with malignant melanoma

  2020cites this paper
• Skin Metabolomics.

  2020cites this paper
• Research progress and perspective in metabolism and metabolomics of psoriasis

  2020cites this paper
• Epidermal sensors for monitoring skin physiology

  2020cites this paper
• Hydrogel-Based Technologies for the Diagnosis of Skin Pathology

  2020cites this paper
• Is it melanoma? Ask my dog!

  2020cites this paper
• Volatolomics: A broad area of experimentation.

  2019cites this paper
• Metabolic and lipidomic investigation of the antiproliferative effects of coronatine against human melanoma cells

  2019cites this paper
• Endogenous and microbial volatile organic compounds in cutaneous health and disease

  2019cites this paper
• Ultrasensitive Sniff-Cam for Biofluorometric-Imaging of Breath Ethanol Caused by Metabolism of Intestinal Flora.

  2019cites this paper
• Untargeted Urinary 1H NMR-Based Metabolomic Pattern as a Potential Platform in Breast Cancer Detection

  2019cites this paper
• Expanding the Understanding of the Heterogeneous Nature of Melanoma with Bioinformatics and Disorder-Based Proteomics.

  2019cites this paper
• Discrimination between the human prostate normal and cancer cell exometabolome by GC-MS

  2018cites this paper
• The Need of External Validation for Metabolomics Predictive Models

  2018cites this paper
• The Volatilome in Metabolomics

  2018cites this paper
• Overoptimism in cross-validation when using partial least squares-discriminant analysis for omics data: a systematic study

  2018cites this paper
• Application of solid-phase microextraction in current biomedical research.

  2018cites this paper
• Metabolomic identification of diagnostic serum-based biomarkers for advanced stage melanoma

  2018cites this paper
• Comparative metabolite profiling of a metastatic and primary melanoma cell line using untargeted metabolomics: A case study.

  2018cites this paper
• Human stem cell metabolomics : headspace volatile gas analysis as an indicator of self-renewal and differentiation status

  2018cites this paper
• A non-invasive approach to explore the discriminatory potential of the urinary volatilome of invasive ductal carcinoma of the breast

  2018cites this paper
• Coupling Large-Scale Omics Data for Deciphering Systems Complexity

  2018cites this paper
• Identification of volatile markers for the detection of adulterants in red ginseng (Panax ginseng) juice using headspace stir-bar sorptive extraction coupled with gas chromatography and mass spectrometry.

  2018cites this paper
• Synergy between nanomaterials and volatile organic compounds for non-invasive medical evaluation.

  2018cites this paper
• Analysis of Volatile Compounds by Advanced Analytical Techniques and Multivariate Chemometrics.

  2017cites this paper
• Non-Invasive Assessment of Skin Barrier Properties: Investigating Emerging Tools for In Vitro and In Vivo Applications

  2017cites this paper
• Application de la volatolomique à la recherche de marqueurs d’exposition aux micropolluants dans le foie, le plasma et le tissu adipeux

  2017cites this paper
• Volatile organic compound and microbiome profiling in patients with colorectal cancer, their spouses and first degree relatives

  2017cites this paper
• Volatile organic compound detection as a potential means of diagnosing cutaneous wound infections

  2017cites this paper
• The metabolomics of psoriatic disease

  2017cites this paper
• Probing skin physiology through the volatile footprint: Discriminating volatile emissions before and after acute barrier disruption

  2017cites this paper
• Discovery of potential biomarkers in human melanoma cells with different metastatic potential by metabolic and lipidomic profiling

  2017cites this paper
• Short overview on metabolomics approach to study pathophysiology of oxidative stress in cancer

  2017cites this paper
• The application of skin metabolomics in the context of transdermal drug delivery

  2017cites this paper
• Novel serum metabolomics‐based approach by gas chromatography/triple quadrupole mass spectrometry for detection of human skin cancers: Candidate biomarkers

  2017cites this paper
• A non-invasive tool for detecting cervical cancer odor by trained scent dogs

  2017cites this paper
• Invasive melanoma in vivo can be distinguished from basal cell carcinoma, benign naevi and healthy skin by canine olfaction: a proof‐of‐principle study of differential volatile organic compound emission

  2016cites this paper
• SPME as a promising tool in translational medicine and drug discovery: From bench to bedside.

  2016cites this paper
• Techniques and issues in breath and clinical sample headspace analysis for disease diagnosis.

  2016cites this paper
• Volatile organic compound markers of psychological stress in skin: a pilot study

  2016cites this paper
• Nuclear Magnetic Resonance metabolomics reveals an excretory metabolic signature of renal cell carcinoma

  2016cites this paper
• Unraveling heterogeneous susceptibility and the evolution of breast cancer using a systems biology approach

  2015cites this paper
• Emerging field of metabolomics: big promise for cancer biomarker identification and drug discovery.

  2015cites this paper
• Dihydrolipoyl dehydrogenase as a potential UVB target in skin epidermis; using an integrated approach of label-free quantitative proteomics and targeted metabolite analysis.

  2015cites this paper
• Hybride Volatolomik und der Nachweis von Krankheiten

  2015cites this paper
• Solid phase microextraction (SPME) method development in analysis of volatile organic compounds (VOCs) as potential biomarkers of cancer

  2015cites this paper
• Profiling of plasma metabolites in canine oral melanoma using gas chromatography-mass spectrometry

  2015cites this paper
• Targeted metabolic profiling of wounds in diabetic and nondiabetic mice

  2015cites this paper
• Hybrid volatolomics and disease detection.

  2015cites this paper
• Solid-phase microextraction in metabolomics

  2014cites this paper
• Characterization of Volatile Organic Metabolites in Lung Cancer Pleural Effusions by SPME–GC/MS Combined with an Untargeted Metabolomic Method

  2014cites this paper
• Recent Development of GC-MS-Based Analytical Procedures of Metabolomics in Screening of Lung Cancer Biomarkers

  2014cites this paper
• Optimisation of Sample Preparation for Direct SPME-GC-MS Analysis of Murine and Human Faecal Volatile Organic Compounds for Metabolomic Studies

  2014cites this paper
• Analysis of volatile human urinary metabolome by solid-phase microextraction in combination with gas chromatography-mass spectrometry for biomarker discovery: application in a pilot study to discriminate patients with renal cell carcinoma.

  2014cites this paper
• Detecting bladder cancer using volatile analyses: is this the future?

  2014cites this paper
• Biomarkers in renal cell carcinoma: a metabolomics approach

  2014cites this paper
• In vivo and ex vivo SPME: a low invasive sampling and sample preparation tool in clinical bioanalysis.

  2014cites this paper
• Subcutaneous Adipocytes Promote Melanoma Cell Growth by Activating the Akt Signaling Pathway

  2014cites this paper
• Recent Development of GC-MS-Based Analytical Procedures of Metabolomics in Screening of Lung Cancer Biomarkers

  2014cites this paper
• Bioinformatics: The Next Frontier of Metabolomics

  2014cites this paper
• 1H NMR metabolomics study of metastatic melanoma in C57BL/6J mouse spleen

  2014cites this paper
• Identi�cation of a colony-and dose-dependent worker aggregation pheromone in the subterranean termite Reticulitermes virginicus

  year unknowncites this paper