Loss of Flight and Associated Neuronal Rhythmicity in Inositol 1,4,5-Trisphosphate Receptor Mutants of Drosophila

Coordinated flight in winged insects requires rhythmic activity of the underlying neural circuit. Here, we show that Drosophila mutants for the inositol 1,4,5-trisphosphate (InsP3) receptor gene (itpr) are flightless. Electrophysiological recordings from thoracic indirect flight muscles show increased spontaneous firing accompanied by a loss of rhythmic flight activity patterns normally generated in response to a gentle puff of air. In contrast, climbing speed, the jump response, and electrical properties of the giant fiber pathway are normal, indicating that general motor coordination and neuronal excitability are much less sensitive to itpr mutations. All mutant phenotypes are rescued by expression of an itpr+ transgene in serotonin and dopamine neurons. Pharmacological and immunohistochemical experiments support the idea that the InsP3 receptor functions to modulate flight specifically through serotonergic interneurons. InsP3 receptor action appears to be important for normal development of the flight circuit and its central pattern generator.

• Publication year

  2004

• Venue

  Journal of Neuroscience

• Publication date

  2004-09-08

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1523/JNEUROSCI.0656-04.2004PMID 15356199PMCID PMC1289272
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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