Transcription Factor-dependent Loading of the E1 Initiator Reveals Modular Assembly of the Papillomavirus Origin Melting Complex*

Replication of bovine papillomavirus type 1 DNA absolutely requires the viral transcription factor E2 as well as the initiator E1, although E1 alone has all the activities expected of an initiator protein. E1 assembles on the DNA in a stepwise fashion and undergoes a transition in activities from site-specific DNA-binding protein to mobile helicase. Complex assembly is assisted by the viral transcription factor E2 at two levels. E2 acts generally as a specificity factor, which through cooperative binding with E1 generates an initial E1 complex containing three E1 dimers bound toori on one face of the DNA, E1-ori. Furthermore, E2 can promote the transition to an orimelting complex by recruiting additional E1 molecules toori, effectively reducing the E1 concentration required forori melting. This reaction is dependent on an E2-binding site positioned distal to the precursor E1-ori complex. The final origin melting complex has two subunits that each encircle the DNA and function independently to melt ori. The assembly pathway we describe has implication for understanding DNA melting and unwinding reactions, which are generally poorly understood.

• Publication year

  2000

• Venue

  Journal of Biological Chemistry

• Publication date

  2000-02-04

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.275.5.3522PMID 10652347
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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