Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources

We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components.

• Publication year

  2012

• Venue

  AMB Express

• Publication date

  2012-07-29

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1186/2191-0855-2-37PMID 22839966PMCID 3444338
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Putative Role of Cellulosomal Protease Inhibitors in Clostridium cellulovorans Based on Gene Expression and Measurement of Activities

  2011cited by this paper
• Insights into higher-order organization of the cellulosome revealed by a dissect-and-build approach: crystal structure of interacting Clostridium thermocellum multimodular components.

  2010cited by this paper
• Comparative genomics of the mesophilic cellulosome‐producing Clostridium cellulovorans and its application to biofuel production via consolidated bioprocessing

  2010cited by this paper
• GENOME ANNOUNCEMENT Genome Sequence of the Cellulosome-Producing Mesophilic Organism Clostridium cellulovorans 743B

  2010cited by this paper
• Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast

  2008cited by this paper
• Monolithic silica columns with various skeleton sizes and through-pore sizes for capillary liquid chromatography.

  2002influential reference
• The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity.

  2001cited by this paper
• Direct analysis of protein complexes using mass spectrometry

  1999cited by this paper
• Effect of skeleton size on the performance of octadecylsilylated continuous porous silica columns in reversed-phase liquid chromatography.

  1997cited by this paper
• Octadecylsilylated porous silica rods as separation media for reversed-phase liquid chromatography.

  1996cited by this paper
• Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase.

  1990cited by this paper
• Isolation and Characterization of an Anaerobic, Cellulolytic Bacterium, Clostridium cellulovorans sp. nov

  1984cited by this paper

• Proteomic Characterization of the Clostridium cellulovorans Cellulosome and Noncellulosomal Enzymes with Sorghum Bagasse

  2025influential citation
• Metabolic Difference Analysis of Clostridium cellulovorans Grown on Glucose and Cellulose

  2023cites this paper
• Enzymatic Characterization of Unused Biomass Degradation Using the Clostridium cellulovorans Cellulosome

  2022cites this paper
• A three-component monooxygenase from Rhodococcus wratislaviensis may expand industrial applications of bacterial enzymes

  2021cites this paper
• Enzyme systems involved in glucosinolate metabolism in Companilactobacillus farciminis KB1089

  2021cites this paper
• Bioconversion of Lignocellulosic Biomass into Value Added Products under Anaerobic Conditions: Insight into Proteomic Studies

  2021cites this paper
• A three-component monooxygenase from Rhodococcus wratislaviensis may expand industrial applications of bacterial enzymes

  2021cites this paper
• Clostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach.

  2020cites this paper
• Improved n-Butanol Production from Clostridium cellulovorans by Integrated Metabolic and Evolutionary Engineering

  2019cites this paper
• Temporal proteome dynamics of Clostridium cellulovorans cultured with major plant cell wall polysaccharides

  2019cites this paper
• Substrate-Related Factors Affecting Cellulosome-Induced Hydrolysis for Lignocellulose Valorization

  2019cites this paper
• Cover letter

  2019cites this paper
• Advantages of proteomics using meter‐long monolithic columns with small inner diameter

  2019cites this paper
• Enzymatic and molecular characterization of an endoglucanase E from Clostridium cellulovorans 743B.

  2019cites this paper
• Evaluation of Unconventional Protein Secretion by Saccharomyces cerevisiae and other Fungi

  2018cites this paper
• Colocalization and Disposition of Cellulosomes in Clostridium clariflavum as Revealed by Correlative Superresolution Imaging

  2018cites this paper
• Protection of Coral Larvae from Thermally Induced Oxidative Stress by Redox Nanoparticles

  2018cites this paper
• Xylanase B from Clostridium cellulovorans 743B: overexpression, purification, crystallization and X-ray diffraction analysis.

  2018cites this paper
• Molecular and Physiological Study of Candida albicans by Quantitative Proteome Analysis

  2018cites this paper
• Characterization of the cellulosomal scaffolding protein CbpC from Clostridium cellulovorans 743B.

  2017cites this paper
• Molecular changes in appearance of a cancer cell among normal HEK293T cells.

  2017cites this paper
• Process engineering of cellulosic n-butanol production from corn-based biomass using Clostridium cellulovorans

  2017cites this paper
• Studies on symbiosis-spesific phenotype of Mesorhizobium loti and its function to host plant

  2017cites this paper
• Definitive screening design enables optimization of LC–ESI–MS/MS parameters in proteomics

  2017cites this paper
• Cellulosomes: bacterial nanomachines for dismantling plant polysaccharides

  2016cites this paper
• Important Roles of the Cellulosome on Degradation of Plant Biomass

  2016cites this paper
• Validation of gibberellin synthetic pathway of rhizobia

  2016cites this paper
• Synergistic properties of cellulases from Clostridium cellulovorans in the presence of cellobiose

  2016cites this paper
• Direct ethanol fermentation of the algal storage polysaccharide laminarin with an optimized combination of engineered yeasts.

  2016cites this paper
• Proposed alginate utilization process of the macroalgae-assimilating Saccharophagus degradans 2-40 based on quantitative proteomic analysis

  2016cites this paper
• Putative Alginate Assimilation Process of the Marine Bacterium Saccharophagus degradans 2-40 Based on Quantitative Proteomic Analysis

  2015cites this paper
• Exoproteome analysis of Clostridium cellulovorans in natural soft-biomass degradation

  2015cites this paper
• Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis

  2015influential citation
• Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis

  2015cites this paper
• Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

  2015cites this paper
• Construction of an efficient degradation system for cellulosic biomass

  2015influential citation
• Metabolic engineering of clostridia for the production of chemicals

  2015cites this paper
• Combining Proteomic Strategies and Molecular Display Technology forDevelopment of Vaccines against Candida albicans

  2014cites this paper
• Feasibilities of consolidated bioprocessing microbes: from pretreatment to biofuel production.

  2014cites this paper
• A Noncellulosomal Mannanase26E Contains a CBM59 in Clostridium cellulovorans

  2014cites this paper
• Expression of Cellulosome Components and Type IV Pili within the Extracellular Proteome of Ruminococcus flavefaciens 007

  2013cites this paper
• Exoproteome Profiles of Clostridium cellulovorans Grown on Various Carbon Sources

  2013cites this paper
• Time-course proteomic profile of Candida albicans during adaptation to a fetal serum.

  2013cites this paper
• Cellulosome Complexes: Natural Biocatalysts as Arming Microcompartments of Enzymes

  2013cites this paper
• Disclosure of the differences of Mesorhizobium loti under the free-living and symbiotic conditions by comparative proteome analysis without bacteroid isolation

  2013cites this paper
• Making headway in understanding pine wilt disease: what do we perceive in the postgenomic era?

  2013cites this paper
• Extracytoplasmic Protein Machines for Macromolecular Degradation

  2013cites this paper