Identification of sympatric cryptic species of Aedes albopictus subgroup in Vietnam: new perspectives in phylosymbiosis of insect vector

The Aedes (Stegomyia) albopictus subgroup includes 11 cryptic species of which Ae. albopictus is the most widely distributed. Its global expansion associated with a documented vector competence for several emerging arboviruses raise obvious concerns in the recently colonized regions. While several studies have provided important insights regarding medical importance of Ae. albopicus, the investigations of the other sibling species are scarce. In Asia, indigenous populations within the Ae. albopictus subgroup can be found in sympatry. In the present study, we aimed to describe and compare molecular, morphological and bacterial symbionts composition among sympatric individuals from the Ae. albopictus subgroup inhabiting a Vietnamese protected area. Based on morphological structure of the cibarial armarture, we identified a cryptic species in the forest park at Bù Gia Mập in the south-eastern region of Vietnam. Analysis of nuclear (ITS1-5.8S-ITS2) and mitochondrial (cox1, nad5) markers confirmed the divergence between the cryptic species and Ae. albopictus. Analysis of midgut bacterial microbiota revealed a strong similarity among the two species with a notable difference; contrary to Ae. albopictus, the cryptic species did not harbour any Wolbachia infection. These results could reflect either a recent invasion of Wolbachia in Ae. albopictus or alternatively a loss of this symbiont in the cryptic species. We argue that neglected species of the Ae. albopictus subgroup are of main importance in order to estimate variation of host-symbionts interactions across evolution.

• Publication year

  2017

• Venue

  Parasites & Vectors

• Publication date

  2017-06-02

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s13071-017-2202-9PMID 28577575PMCID 5457575
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Coexisting cryptic species of the Litoditis marina complex (Nematoda) show differential resource use and have distinct microbiomes with high intraspecific variability

  2016cited by this paper
• Gut Microbiotas and Host Evolution: Scaling Up Symbiosis.

  2016cited by this paper
• Culex pipiens and Culex restuans mosquitoes harbor distinct microbiota dominated by few bacterial taxa

  2016cited by this paper
• Molecular data reveal a cryptic species within the Culex pipiens mosquito complex

  2016cited by this paper
• French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives

  2015influential reference
• The Insect Microbiome Modulates Vector Competence for Arboviruses

  2014cited by this paper
• Pyrosequencing 16S rRNA genes of bacteria associated with wild tiger mosquito Aedes albopictus: a pilot study

  2014cited by this paper
• The role of environmental, virological and vector interactions in dictating biological transmission of arthropod-borne viruses by mosquitoes.

  2014cited by this paper
• Harnessing mosquito-Wolbachia symbiosis for vector and disease control.

  2014cited by this paper
• Composition of Anopheles coluzzii and Anopheles gambiae microbiota from larval to adult stages.

  2014cited by this paper
• Arboviral Bottlenecks and Challenges to Maintaining Diversity and Fitness during Mosquito Transmission

  2014cited by this paper
• The Hologenomic Basis of Speciation: Gut Bacteria Cause Hybrid Lethality in the Genus Nasonia

  2013cited by this paper
• Diversity and function of bacterial microbiota in the mosquito holobiont

  2013cited by this paper
• Bacterial Communities Associated with Culex Mosquito Larvae and Two Emergent Aquatic Plants of Bioremediation Importance

  2013cited by this paper
• The gut microbiota of insects - diversity in structure and function.

  2013cited by this paper
• Wolbachia Infections Mimic Cryptic Speciation in Two Parasitic Butterfly Species, Phengaris teleius and P. nausithous (Lepidoptera: Lycaenidae)

  2013cited by this paper
• Spatiotemporal Dynamics of Dengue Epidemics, Southern Vietnam

  2013cited by this paper
• PANDAseq: paired-end assembler for illumina sequences

  2012cited by this paper
• Biogeography of the two major arbovirus mosquito vectors, Aedes aegypti and Aedes albopictus (Diptera, Culicidae), in Madagascar

  2012cited by this paper
• MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space

  2012cited by this paper
• CircadiOmics: integrating circadian genomics, transcriptomics, proteomics and metabolomics

  2012cited by this paper
• Diversification of Wolbachia endosymbiont in the Culex pipiens mosquito.

  2011cited by this paper
• Diversity and Antibiotic Resistance Patterns of Sphingomonadaceae Isolates from Drinking Water

  2011cited by this paper
• Bacterial diversity of field-caught mosquitoes, Aedes albopictus and Aedes aegypti, from different geographic regions of Madagascar.

  2011cited by this paper
• High prevalence and lack of diversity of Wolbachia pipientis in Aedes albopictus populations from Northeast Brazil.

  2011cited by this paper
• Comparisons of host mitochondrial, nuclear and endosymbiont bacterial genes reveal cryptic fig wasp species and the effects of Wolbachia on host mtDNA evolution and diversity

  2011cited by this paper
• Partial mitochondrial DNA sequences suggest the existence of a cryptic species within the Leucosphyrus group of the genus Anopheles (Diptera: Culicidae), forest malaria vectors, in northern Vietnam

  2010cited by this paper
• SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building.

  2010cited by this paper
• A multiplex PCR-based molecular identification of five morphologically related, medically important subgenus Stegomyia mosquitoes from the genus Aedes (Diptera: Culicidae) found in the Ryukyu Archipelago, Japan.

  2010cited by this paper
• Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial vector

  2009cited by this paper
• Quantitative Community Fingerprinting Methods for Estimating the Abundance of Operational Taxonomic Units in Natural Microbial Communities

  2009cited by this paper
• DnaSP v5: a software for comprehensive analysis of DNA polymorphism data

  2009cited by this paper
• Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities

  2009cited by this paper
• Naïve Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy

  2007cited by this paper
• Evolutionary history of a mosquito endosymbiont revealed through mitochondrial hitchhiking

  2006cited by this paper
• Survival of Wolbachia pipientis in Cell-Free Medium

  2006cited by this paper
• Molecular and Morphological Characterization of Aedes albopictus in Northwestern Greece and Differentiation from Aedes cretinus and Aedes aegypti

  2006cited by this paper
• Problems with mitochondrial DNA as a marker in population, phylogeographic and phylogenetic studies: the effects of inherited symbionts

  2005cited by this paper
• Comparison of Different Primer Sets for Use in Automated Ribosomal Intergenic Spacer Analysis of Complex Bacterial Communities

  2004cited by this paper
• MUSCLE: multiple sequence alignment with high accuracy and high throughput.

  2004cited by this paper
• Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with Dengue Virus Transmission

  2004cited by this paper
• Mutualistic Wolbachia infection in Aedes albopictus: accelerating cytoplasmic drive.

  2002cited by this paper
• Maternal transmission efficiency of Wolbachia superinfections in Aedes albopictus populations in Thailand.

  2002cited by this paper
• Wolbachia-induced incompatibility precedes other hybrid incompatibilities in Nasonia

  2001cited by this paper
• Phylogeny and PCR–based classification of Wolbachia strains using wsp gene sequences

  1998cited by this paper
• The biology of Aedes albopictus.

  1988cited by this paper
• The biology of Aedes albopictus.

  1988cited by this paper
• Intraspecific and interspecific variation in the sequence and abundance of highly repeated DNA among mosquitoes of the Aedes albopictus subgroup

  1987cited by this paper
• Medical Entomology Studies - XI. The Subgenus Stegomyia of Aedes in the Oriental Region with Keys to the Species (Diptera: Culicidae)

  1979cited by this paper
• A Revision of the Adult and Larval Mosquitoes of Japan (Including the Ryukyu Archipelago and the Ogasawara Islands) and Korea (Diptera: Culicidae)

  1979cited by this paper
• The Female Cibarial Armature of New World Culex, Subgenus Melanoconion and Related Subgenera with Notes on this Character in Subgenera Culex, Lutzia and Neoculex and Genera Galindomyia and Deinocerites (Diptera: Culicidae)

  1978cited by this paper
• Contributions to the Mosquito Fauna of Southeast Asia. XIV. The Subgenus Stegomyia of Aedes in Southeast Asia I - The Scutellaris Group of Species

  1972cited by this paper

• Thermal stress responses on developmental time, survival, and wing morphometrics in desert and coastal strains of Aedes aegypti (Diptera: Culicidae) from India.

  2026cites this paper
• Seasonal variation of Microsporidia MB infection in Anopheles gambiae and Anopheles coluzzii in two different geographical localities in Benin

  2025cites this paper
• Chromosome-scale genome assembly of the South American fruit fly, Anastrepha fraterculus sp.1.

  2025cites this paper
• Blood meal modulates midgut bacterial community structure and metabolic function in Aedes albopictus.

  2025cites this paper
• The interplay between species and locations shapes vector fleas microbial communities in plague foci: pathogens rather than symbionts?

  2025cites this paper
• Detection of a sympatric cryptic species mimicking Aedes albopictus (Diptera: Culicidae) in dengue and Chikungunya endemic forest villages of Tripura, India, posing a daunting challenge for vector research

  2025influential citation
• Aedes (Ochlerotatus) scapularis, Aedes japonicus japonicus, and Aedes (Fredwardsius) vittatus (Diptera: Culicidae): Three Neglected Mosquitoes with Potential Global Health Risks

  2024cites this paper
• Aedes unalom sp. nov. in Cambodia, a new Stegomyia species close to Aedes albopictus (Diptera, Culicidae)

  2024cites this paper
• DNA Barcoding Analysis of Trinidad Haemagogus Mosquitoes Reveals Evidence for Putative New Species

  2024cites this paper
• The origin and insecticide resistance of Aedes albopictus mosquitoes established in southern Mozambique

  2024cites this paper
• Microbiome Profile of South Korean Vector Mosquitoes.

  2024cites this paper
• Low coverage of species constrains the use of DNA barcoding to assess mosquito biodiversity

  2024cites this paper
• Population genetic characterization of (Aedes albopictus) mosquitoes (Diptera: Culicidae) from the Yangtze River Basin of China based on rDNA-ITS2.

  2023cites this paper
• Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast India

  2023cites this paper
• Molecular techniques for the taxonomy of Aedes Meigen, 1818 (Culicidae: Aedini): a review of studies from 2010 to 2021.

  2022cites this paper
• Sex-specific distribution and classification of Wolbachia infections and mitochondrial DNA haplogroups in Aedes albopictus from the Indo-Pacific

  2022cites this paper
• A Barcoding and Morphological Identification of Mosquito Species of the Genus Aedes (Diptera: Culicidae) of the Russian Far East and Northern Vietnam

  2022cites this paper
• Seasonal variation of microbiota composition in Anopheles gambiae and Anopheles coluzzii in two different eco‐geographical localities in Cameroon

  2022cites this paper
• Description of Culex (Culex) kodaikanalensis sp. nov. (Diptera: Culicidae) from India with phyletic analysis using mitochondrial cytochrome oxidase I and 16S rRNA genes

  2022cites this paper
• Culex (Culex) gaugleri, a new species (Diptera: Culicidae) from India

  2022cites this paper
• Aedes albopictus diversity and relationships in south-western Europe and Brazil by rDNA/mtDNA and phenotypic analyses: ITS-2, a useful marker for spread studies

  2021cites this paper
• Unveiling mosquito cryptic species and their reproductive isolation

  2020influential citation
• Comparative Phylogeography and Integrative Taxonomy of Ochlerotatus caspius (Dipera: Culicidae) and Ochlerotatus dorsalis

  2020cites this paper
• Two Distinct Genotypes of Spissistilus festinus (Say, 1830) (Hemiptera, Membracidae) in the United States Revealed by Phylogenetic and Morphological Analyses

  2020cites this paper
• Use of Microbiota to Fight Mosquito-Borne Disease

  2020cites this paper
• Considerations for mosquito microbiome research from the Mosquito Microbiome Consortium

  2020cites this paper
• Taxonomical over splitting in the Rhodnius prolixus (Insecta: Hemiptera: Reduviidae) clade: Are R. taquarussuensis (da Rosa et al., 2017) and R. neglectus (Lent, 1954) the same species?

  2019cites this paper
• The mosquito holobiont: fresh insight into mosquito-microbiota interactions

  2018influential citation
• Parasite Biology: The Vectors

  2018cites this paper
• Molecular evidence for new sympatric cryptic species of Aedes albopictus (Diptera: Culicidae) in China: A new threat from Aedes albopictus subgroup?

  2018cites this paper
• DNA barcoding as a complementary approach for species identification from dengue endemic regions of North East India

  2018cites this paper
• Mapping the global potential distributions of two arboviral vectors Aedes aegypti and Ae. albopictus under changing climate

  2018cites this paper
• Short-term impacts of anthropogenic stressors on Aedes albopictus mosquito vector microbiota.

  2018cites this paper
• Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA

  2017cites this paper