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Use of the comet assay technique for quick and reliable prediction of in vitro response to chemotherapeutics in breast and colon cancer

P. Apostolou,M. Toloudi,Eleni Kourtidou,G. Mimikakou,I. Vlachou,M. Chatziioannou,I. Papasotiriou

Published 2014 in Journal of Biological Research - Thessaloniki

ABSTRACT

BackgroundDetermination of response to chemotherapy is a major requirement of personalized medicine. Resistance, whether developed or native, critically affects a treatment’s success. Single Cell Gel lectrophoresis - also known as a comet assay - is used to detect DNA damage at the level of individual eukaryotic cells. We assessed the use of comet assays in determining response to chemotherapeutic drugs that are widely used in breast and colon cancer.ResultsWe treated human breast and colon cancer cell lines with melphalan, cisplatin, mechlorethamine or doxorubicin, as monotherapies. Drug activities varied even in the same cancer types, further demonstrating the heterogeneity of different cancer types.ConclusionThe comet assay technique can provide reliable and quick results with minimum requirements and is applicable to a wide variety of drugs.

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