BackgroundThe main control strategy for visceral leishmaniasis in Brazil has been based on the elimination of seropositive dogs, although this is not widely accepted. In this context, the use of a long-lasting protective vaccine against canine visceral leishmaniasis (CVL) has been highly expected. The aim of this work was to determine the timeline kinetics of the cytokine microenvironment derived from circulating leukocytes as supportive immunological biomarkers triggered by Leishmune® vaccine. Cross-sectional kinetic analysis of cellular immunity cytokines was carried out at three times (1, 6 and 12 months) after primovaccination with Leishmune®. In vitro short-term whole blood cultures were stimulated with Leishmania infantum soluble antigen (SLAg). The secreted cytokine signatures and their major sources were determined.ResultsAt six months after vaccination, Leishmune® induced an increase in IL-8, IFN-γ, IL-17a and TNF-α levels and a decrease in IL-10. Cytokine signature analysis revealed a shift in the microenvironment towards a pro-inflammatory profile mediated by IL-8 and IFN-γ. Both, CD4+ (↑TNF-α+ and ↑IFN-γ +) and CD8+ (↑IL-17a and ↓IL-4) T-cells contributed to the acquired immune responses observed after stimulation with SLAg.ConclusionsThe changes observed in the cytokine profile suggested that Leishmune® was able to induce an effective response at six months after primovaccination. After one year, it returned to baseline suggesting the need of additional boosting.
One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis
Christiane Costa-Pereira,Marcela Moreira,R. Soares,Bruno H Marteleto,V. Ribeiro,Michelle H França-Dias,L. M. Cardoso,K. Viana,R. Giunchetti,O. Martins-Filho,M. Araújo
Published 2015 in BMC Veterinary Research
ABSTRACT
PUBLICATION RECORD
- Publication year
2015
- Venue
BMC Veterinary Research
- Publication date
2015-04-11
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- canine visceral leishmaniasis
A dog disease caused by Leishmania infection that is the target condition for vaccination in this work.
Aliases: CVL
- cd4+ t cells
A T-cell subset whose cytokine-producing response was assessed after antigen stimulation.
Aliases: CD4 T cells, helper T cells
- cd8+ t cells
A T-cell subset whose cytokine-producing response was assessed after antigen stimulation.
Aliases: CD8 T cells, cytotoxic T cells
- cytokine microenvironment
The circulating leukocyte-derived cytokine milieu measured as an immunological readout in vaccinated dogs.
Aliases: cytokine milieu, cytokine profile
- ifn-γ
A cytokine measured in stimulated blood cultures as part of the immune response profile.
Aliases: interferon gamma, interferon-γ
- il-10
A cytokine measured in stimulated blood cultures as part of the immune response profile.
Aliases: interleukin-10
- il-17a
A cytokine measured in stimulated blood cultures as part of the immune response profile.
Aliases: interleukin-17a
- il-4
A cytokine measured in stimulated blood cultures as part of the T-cell response profile.
Aliases: interleukin-4
- il-8
A cytokine measured in stimulated blood cultures as part of the immune response profile.
Aliases: interleukin-8
- leishmune® vaccine
A long-lasting vaccine candidate administered to dogs as primovaccination in this study.
Aliases: Leishmune, Leishmune vaccine
- primovaccination
The initial vaccination phase used as the starting point for the one-year kinetic follow-up.
Aliases: primary vaccination, initial vaccination
- pro-inflammatory profile
A cytokine pattern characterized by inflammatory signaling rather than a suppressive immune state.
Aliases: proinflammatory profile
- soluble leishmania infantum antigen (slag)
A Leishmania infantum antigen preparation used to stimulate whole-blood cultures ex vivo.
Aliases: SLAg, Leishmania infantum soluble antigen
- tnf-α
A cytokine measured in stimulated blood cultures as part of the immune response profile.
Aliases: tumor necrosis factor alpha, tumor necrosis factor-α
REFERENCES
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