PtdIns 3-Kinase Orchestrates Autophagosome Formation in Yeast

Eukaryotic cells can massively transport their own cytoplasmic contents into a lytic compartment, the vacuole/lysosome, for recycling through a conserved system called autophagy. The key process in autophagy is the sequestration of cytoplasmic contents within a double-membrane structure, the autophagosome. Autophagosome formation requires the elaborate cooperation of Atg (autophagy-related) proteins and lipid molecules. Phosphorylation of phosphatidylinositol (PtdIns) by a PtdIns 3-kinase, Vps34, is a key step in coordinating Atg proteins and lipid molecules. Vps34 forms two distinct protein complexes, only one of which is involved in generating autophagic membranes. Upon induction of autophagy, PtdIns(3)P, the enzymatic product of PtdIns 3-kinase, is massively transported into the lumen of the vacuole via autophagy. PtdIns(3)P is enriched on the inner membrane of the autophagosome. PtdIns(3)P recruits the Atg18−Atg2 complex and presumably other Atg proteins to autophagic membranes, thereby coordinating lipid molecules and Atg proteins.

• Publication year

  2011

• Venue

  Journal of Lipids

• Publication date

  2011-01-04

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1155/2011/498768PMID 21490802PMCID 3067060
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Regulation of membrane biogenesis in autophagy via PI3P dynamics.

  2010cited by this paper
• Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L

  2010cited by this paper
• Roles of the Lipid-binding Motifs of Atg18 and Atg21 in the Cytoplasm to Vacuole Targeting Pathway and Autophagy*

  2010cited by this paper
• Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation

  2010cited by this paper
• Modulation of Local PtdIns3P Levels by the PI Phosphatase MTMR3 Regulates Constitutive Autophagy

  2010cited by this paper
• Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins

  2010influential reference
• Distinct regulation of autophagic activity by Atg14L and Rubicon associated with Beclin 1–phosphatidylinositol-3-kinase complex

  2009cited by this paper
• Control of autophagy initiation by phosphoinositide 3‐phosphatase jumpy

  2009cited by this paper
• Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages

  2009cited by this paper
• An overview of the molecular mechanism of autophagy.

  2009cited by this paper
• Coordination of membrane events during autophagy by multiple class III PI3-kinase complexes

  2009cited by this paper
• Dynamics and diversity in autophagy mechanisms: lessons from yeast

  2009cited by this paper
• A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation

  2009cited by this paper
• Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase

  2008cited by this paper
• The Atg18-Atg2 Complex Is Recruited to Autophagic Membranes via Phosphatidylinositol 3-Phosphate and Exerts an Essential Function*

  2008cited by this paper
• Dynamics and function of PtdIns(3)P in autophagy

  2008cited by this paper
• Bcl-2 family members: dual regulators of apoptosis and autophagy.

  2008cited by this paper
• Atg8 controls phagophore expansion during autophagosome formation.

  2008cited by this paper
• Transport of phosphatidylinositol 3‐phosphate into the vacuole via autophagic membranes in Saccharomyces cerevisiae

  2008cited by this paper
• Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum

  2008cited by this paper
• RNAi-mediated chromatin silencing in fission yeast.

  2008cited by this paper
• [Key questions about membrane dynamics during autophagy].

  2008cited by this paper
• Bcl-2 family members: Dual regulators of apoptosis and autophagy

  2008influential reference
• Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG.

  2008cited by this paper
• FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells

  2008cited by this paper
• Atg18 regulates organelle morphology and Fab1 kinase activity independent of its membrane recruitment by phosphatidylinositol 3,5-bisphosphate.

  2007cited by this paper
• Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion.

  2007cited by this paper
• The Atg12-Atg5 Conjugate Has a Novel E3-like Activity for Protein Lipidation in Autophagy*

  2007cited by this paper
• Hierarchy of Atg proteins in pre‐autophagosomal structure organization

  2007cited by this paper
• Autophagy: process and function.

  2007cited by this paper
• Ambra1 regulates autophagy and development of the nervous system

  2007cited by this paper
• Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice

  2006cited by this paper
• Loss of autophagy in the central nervous system causes neurodegeneration in mice

  2006cited by this paper
• Assortment of phosphatidylinositol 3-kinase complexes--Atg14p directs association of complex I to the pre-autophagosomal structure in Saccharomyces cerevisiae.

  2006cited by this paper
• The relevance of the phosphatidylinositolphosphat‐binding motif FRRGT of Atg18 and Atg21 for the Cvt pathway and autophagy

  2006cited by this paper
• Svp1p defines a family of phosphatidylinositol 3,5‐bisphosphate effectors

  2004cited by this paper
• Autophagy Defends Cells Against Invading Group A Streptococcus

  2004cited by this paper
• Atg21 is a phosphoinositide binding protein required for efficient lipidation and localization of Atg8 during uptake of aminopeptidase I by selective autophagy.

  2004cited by this paper
• A unified nomenclature for yeast autophagy-related genes.

  2003cited by this paper
• Cooperative Binding of the Cytoplasm to Vacuole Targeting Pathway Proteins, Cvt13 and Cvt20, to Phosphatidylinositol 3-Phosphate at the Pre-autophagosomal Structure Is Required for Selective Autophagy*

  2002cited by this paper
• Mai1p is essential for maturation of proaminopeptidase I but not for autophagy

  2002cited by this paper
• All Phox Homology (PX) Domains from Saccharomyces cerevisiae Specifically Recognize Phosphatidylinositol 3-Phosphate*

  2001cited by this paper
• The pre‐autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation

  2001cited by this paper
• Two Distinct Vps34 Phosphatidylinositol 3–Kinase Complexes Function in Autophagy and Carboxypeptidase Y Sorting inSaccharomyces cerevisiae

  2001cited by this paper
• Dissection of Autophagosome Formation Using Apg5-Deficient Mouse Embryonic Stem Cells

  2001cited by this paper
• Localization of phosphatidylinositol 3‐phosphate in yeast and mammalian cells

  2000cited by this paper
• A ubiquitin-like system mediates protein lipidation

  2000cited by this paper
• Apg9p/Cvt7p Is an Integral Membrane Protein Required for Transport Vesicle Formation in the Cvt and Autophagy Pathways

  2000cited by this paper
• The Reversible Modification Regulates the Membrane-Binding State of Apg8/Aut7 Essential for Autophagy and the Cytoplasm to Vacuole Targeting Pathway

  2000cited by this paper
• Distinct Classes of Phosphatidylinositol 3′-Kinases Are Involved in Signaling Pathways That Control Macroautophagy in HT-29 Cells*

  2000cited by this paper
• LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing

  2000cited by this paper
• Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast

  1999cited by this paper
• Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains.

  1998cited by this paper
• Apg14p and Apg6/Vps30p Form a Protein Complex Essential for Autophagy in the Yeast, Saccharomyces cerevisiae *

  1998cited by this paper
• Analysis of the membrane structures involved in autophagy in yeast by freeze-replica method.

  1995cited by this paper
• Wortmannin causes mistargeting of procathepsin D. evidence for the involvement of a phosphatidylinositol 3-kinase in vesicular transport to lysosomes

  1995cited by this paper
• Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway

  1995cited by this paper
• Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast

  1995cited by this paper
• Role for phosphatidylinositol 3-kinase in the sorting and transport of newly synthesized lysosomal enzymes in mammalian cells

  1995cited by this paper
• Ultrastructural analysis of the autophagic process in yeast: detection of autophagosomes and their characterization

  1994cited by this paper
• Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities.

  1994cited by this paper
• Isolation of autophagocytosis mutants of Saccharomyces cerevisiae

  1994cited by this paper
• Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting.

  1993cited by this paper
• Isolation and characterization of autophagy‐defective mutants of Saccharomyces cerevisiae

  1993cited by this paper
• Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway

  1992cited by this paper
• A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein delivery.

  1991cited by this paper
• Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases

  1988cited by this paper

• Mass Spectrometry Detects Sphingolipid Metabolites for Discovery of New Strategy for Cancer Therapy from the Aspect of Programmed Cell Death

  2023cites this paper
• The Regulation of Exosome Generation and Function in Physiological and Pathological Processes

  2023cites this paper
• Role of ceramides and sphingolipidsin Parkinson's disease.

  2023cites this paper
• The basic functions of phosphoglycerate kinase 1 and its roles in cancer and other diseases.

  2022cites this paper
• Role of Bisphenol A in Autophagy Modulation: Understanding the Molecular Concepts and Therapeutic Options.

  2022cites this paper
• A dynamically evolving war between autophagy and pathogenic microorganisms

  2022cites this paper
• Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light

  2022cites this paper
• Characterization of Protein–Membrane Interactions in Yeast Autophagy

  2022cites this paper
• Prediction of cardiovascular risk by measuring carotid intima media thickness from an ultrasound image for type II diabetic mellitus subjects using machine learning and transfer learning techniques

  2021cites this paper
• Senecio serratuloides extract prevents the development of hypertension, oxidative stress and dyslipidemia in nitric oxide-deficient rats

  2020cites this paper
• Autophagy and the Wnt Signaling Pathway: A Focus on Wnt/β-catenin signaling.

  2020cites this paper
• Pexophagy Protects Plants from Reactive Oxygen Species-induced Damage under High-intensity Light

  2020cites this paper
• Autophagy and cancer therapy cardiotoxicity: From molecular mechanisms to therapeutic opportunities.

  2020cites this paper
• Nitrogen Starvation and Stationary Phase Lipophagy Have Distinct Molecular Mechanisms

  2020cites this paper
• Membrane Contact Sites and Organelles Interaction in Plant Autophagy

  2020cites this paper
• GTP hydrolysis by Synechocystis IM30 does not decisively affect its membrane remodeling activity

  2020cites this paper
• Signaling and other functions of lipids in autophagy: a review

  2020cites this paper
• Extracellular Vesicles as Delivery Vehicles of Specific Cellular Cargo

  2020cites this paper
• Autophagy in Stationary Phase of Growth

  2019cites this paper
• ER platforms mediating autophagosome generation.

  2019cites this paper
• Fusion of Bipolar Tetraether Lipid Membranes Without Enhanced Leakage of Small Molecules

  2019cites this paper
• Exploring the roles of phosphoinositides in the biology of the malaria parasite Plasmodium falciparum

  2019cites this paper
• Separating extracellular vesicles and lipoproteins via acoustofluidics.

  2019cites this paper
• Hydroxyurea alters hematological, biochemical and inflammatory biomarkers in Brazilian children with SCA: Investigating associations with βS haplotype and α-thalassemia

  2019cites this paper
• Lipids in South Asians: Epidemiology and Management

  2019cites this paper
• Changes in expression of the autophagy-related genes microtubule-associated protein 1 light chain 3β and autophagy related 7 in skeletal muscle of fattening Japanese Black cattle: a pilot study

  2018cites this paper
• Autophagy: An Essential Degradation Program for Cellular Homeostasis and Life

  2018cites this paper
• Human iPSC-based models highlight defective glial and neuronal differentiation from neural progenitor cells in metachromatic leukodystrophy

  2018cites this paper
• Dissecting the molecular function of the ubiquitin-like Atg8 during autophagosome biogenesis in S. cerevisiae

  2018cites this paper
• Observational study of the status of coronary risk biomarkers among Negritos with metabolic syndrome in the east coast of Malaysia

  2018cites this paper
• Autophagy and Protein Turnover Responses to Exercise-Nutrient Interactions in Human Skeletal Muscle

  2017cites this paper
• Phosphoinositides, Major Actors in Membrane Trafficking and Lipid Signaling Pathways

  2017cites this paper
• Non-Covalent Assembly Method that Simultaneously Endows a Liposome Surface with Targeting Ligands, Protective PEG Chains, and Deep-Red Fluorescence Reporter Groups.

  2017cites this paper
• A barley homolog of yeast ATG6 is involved in multiple abiotic stress responses and stress resistance regulation.

  2017cites this paper
• Endocytosis of AtRGS1 Is Regulated by the Autophagy Pathway after D-Glucose Stimulation

  2017cites this paper
• Homeostatic Model Assessment-Insulin Resistance (HOMA-IR 2) in Mild Subclinical Hypothyroid Subjects

  2017cites this paper
• High-fructose corn syrup-55 consumption alters hepatic lipid metabolism and promotes triglyceride accumulation.

  2017cites this paper
• Formation of Cell Membrane Component Domains in Artificial Lipid Bilayer

  2017cites this paper
• Phosphatidylinositol‐3‐phosphate in the regulation of autophagy membrane dynamics

  2017cites this paper
• Network and role analysis of autophagy in Phytophthora sojae

  2017cites this paper
• The Autophagic Machinery in Enterovirus Infection

  2016cites this paper
• Autophagy and the nutritional signaling pathway

  2016cites this paper
• Impaired Autophagy and Defective Mitochondrial Function: Converging Paths on the Road to Motor Neuron Degeneration

  2016cites this paper
• Phosphatidylinositol-3-phosphate regulates response of cells to proteotoxic stress.

  2016cites this paper
• Autophagy in plant pathogenic fungi.

  2016cites this paper
• Changes of fatty acids in phosphatidylcholine on sperm membrane during Macrobrachium rosenbergii sperm transit through spermatic duct and lipid analysis in spermatic vesicles

  2016cites this paper
• Postprandial triglyceride-rich lipoproteins regulate perilipin-2 and perilipin-3 lipid-droplet-associated proteins in macrophages.

  2015cites this paper
• Linking the ceramide synthases (CerSs) 4 and 5 with apoptosis, endometrial and colon cancers.

  2015cites this paper
• n-3 Polyunsaturated fatty acids inhibit Fc ε receptor I-mediated mast cell activation.

  2015cites this paper
• WITHDRAWN: Investigating the link of ceramide synthases (CerSes) 4 and 5 in endometrial and colon cancers.

  2015cites this paper
• Syddansk Universitet Autophagy in the light of sphingolipid metabolism

  2015cites this paper
• Autophagy signal transduction by ATG proteins: from hierarchies to networks

  2015cites this paper
• Human WIPIs as Phosphoinositide Effectors at the Nascent Autophagosome: A Robust Tool to Assess Macroautophagy by Quantitative Imaging

  2015cites this paper
• Autophagy in the light of sphingolipid metabolism

  2015cites this paper
• Studies on the response to, and recovery from, rapamycin in Saccharomyces cerevisiae

  2015cites this paper
• C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy.

  2015cites this paper
• Lipid kinases are essential for apicoplast homeostasis in Toxoplasma gondii

  2015cites this paper
• Autophagic kinases SmVPS34 and SmVPS15 are required for viability in the filamentous ascomycete Sordaria macrospora.

  2014cites this paper
• Ceramide synthase inhibitor fumonisin B1 inhibits apoptotic cell death in SCC17B human head and neck squamous carcinoma cells after Pc4 photosensitization

  2014cites this paper
• Vmp1 Regulates PtdIns3P Signaling During Autophagosome Formation in Dictyostelium discoideum

  2014cites this paper
• Characterisation of the role of autophagy in DNA damage repair

  2014cites this paper
• Lipid droplet and early autophagosomal membrane targeting of Atg2A and Atg14L in human tumor cells[S]

  2014cites this paper
• The role of phosphatidylinositol 3-kinases in autophagy regulation

  2014cites this paper
• Atg 38 is required for autophagy-specific phosphatidylinositol 3-kinase complex integrity

  2013cites this paper
• HIV Protease Inhibitors Disrupt Lipid Metabolism by Activating Endoplasmic Reticulum Stress and Inhibiting Autophagy Activity in Adipocytes

  2013cites this paper
• Atg38 is required for autophagy-specific phosphatidylinositol 3-kinase complex integrity

  2013cites this paper
• Increased killing of SCCVII squamous cell carcinoma cells after the combination of Pc 4 photodynamic therapy and dasatinib is associated with enhanced caspase-3 activity and ceramide synthase 1 upregulation

  2013cites this paper
• ULK1 induces autophagy by phosphorylating Beclin-1 and activating Vps34 lipid kinase

  2013cites this paper
• The role of lipids in the control of autophagy.

  2013influential citation
• Triennial Reproduction Symposium: the obstacle course to successful establishment of pregnancy in domestic livestock species.

  2013cites this paper
• Sex Differences in the Hepatic Cholesterol Sensing Mechanisms in Mice

  2013cites this paper
• Differential regulation of distinct Vps34 complexes by AMPK in nutrient stress and autophagy.

  2013cites this paper
• Fructus Xanthii Attenuates Hepatic Steatosis in Rats Fed on High-Fat Diet

  2013cites this paper
• WIPI β-propellers in autophagy-related diseases and longevity.

  2013cites this paper
• A combined fluorescence spectroscopy, confocal and 2-photon microscopy approach to re-evaluate the properties of sphingolipid domains.

  2013cites this paper
• Role of Human WIPIs in Macroautophagy

  2013cites this paper
• A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy

  2013cites this paper
• Signaling by the phosphoinositide 3-kinase family in immune cells.

  2013cites this paper
• mVps34 deletion in podocytes causes glomerulosclerosis by disrupting intracellular vesicle trafficking.

  2013cites this paper
• Characterization of Atg18p and its role in cellular trafficking in Saccharomyces cerevisiae

  2013influential citation
• Insights into membrane binding of PROPPINs and reconstitution of the mammalian autophagic conjugation systems.

  2013cites this paper
• Certain Strongylocentrotus purpuratus sperm mitochondrial proteins co-purify with low density detergent-insoluble membranes and are PKA or PKC-substrates possibly involved in sperm motility regulation.

  2013cites this paper
• Atg 38 is required for autophagy-specific phosphatidylinositol 3-kinase complex integrity

  2013cites this paper
• Atg 38 is required for autophagy-specific phosphatidylinositol 3-kinase complex integrity

  2013cites this paper
• Pexophagy: The Selective Degradation of Peroxisomes

  2012cites this paper
• Consumption of a grape extract supplement containing resveratrol decreases oxidized LDL and ApoB in patients undergoing primary prevention of cardiovascular disease: a triple-blind, 6-month follow-up, placebo-controlled, randomized trial.

  2012cites this paper
• Characterization of the mechanisms underlying alterations in macroautophagy and survival signalling in Huntington’s disease

  2012cites this paper
• Defining regulatory and phosphoinositide-binding sites in the human WIPI-1 β-propeller responsible for autophagosomal membrane localization downstream of mTORC1 inhibition

  2012cites this paper
• How phosphoinositide 3-phosphate controls growth downstream of amino acids and autophagy downstream of amino acid withdrawal.

  2012cites this paper
• KCS1 deletion in Saccharomyces cerevisiae leads to a defect in translocation of autophagic proteins and reduces autophagosome formation

  2012cites this paper
• Rational use of antioxidants in solid oral pharmaceutical preparations

  2012cites this paper
• Recent advances in cystic fibrosis.

  2012cites this paper
• LC3-Associated Phagocytosis (LAP): Connections with Host Autophagy

  2012cites this paper
• Functional similarity analysis of human virus-encoded miRNAs

  2011cites this paper
• A unique way to form a vesicle: aminopeptidase 1 aggregation and its binding to receptor ATG19 for recruitment of autophagic proteins to form a vesicle in the cytoplasm-to-vacuole-targeting pathway in yeast

  2011cites this paper
• Atg14: A Key Player in Orchestrating Autophagy

  2011cites this paper