Soybeans Grown in the Chernobyl Area Produce Fertile Seeds that Have Increased Heavy Metal Resistance and Modified Carbon Metabolism

Plants grow and reproduce in the radioactive Chernobyl area, however there has been no comprehensive characterization of these activities. Herein we report that life in this radioactive environment has led to alteration of the developing soybean seed proteome in a specific way that resulted in the production of fertile seeds with low levels of oil and β-conglycinin seed storage proteins. Soybean seeds were harvested at four, five, and six weeks after flowering, and at maturity from plants grown in either non-radioactive or radioactive plots in the Chernobyl area. The abundance of 211 proteins was determined. The results confirmed previous data indicating that alterations in the proteome include adaptation to heavy metal stress and mobilization of seed storage proteins. The results also suggest that there have been adjustments to carbon metabolism in the cytoplasm and plastids, increased activity of the tricarboxylic acid cycle, and decreased condensation of malonyl-acyl carrier protein during fatty acid biosynthesis.

• Publication year

  2012

• Venue

  PLoS ONE

• Publication date

  2012-10-26

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1371/journal.pone.0048169PMID 23110204PMCID 3482187
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• On the Discordance of Metabolomics with Proteomics and Transcriptomics: Coping with Increasing Complexity in Logic, Chemistry, and Network Interactions Scientific Correspondence

  2012cited by this paper
• Nucleotide diversity and gene expression of Catalase and Glutathione peroxidase in irradiated Scots pine (Pinus sylvestris L.) from the Chernobyl exclusion zone.

  2012cited by this paper
• Metabolic and phenotypic responses of greenhouse-grown maize hybrids to experimentally controlled drought stress.

  2012cited by this paper
• Using proteomics to study sexual reproduction in angiosperms

  2011cited by this paper
• Dynamic metabonomic responses of tobacco (Nicotiana tabacum) plants to salt stress.

  2011cited by this paper
• Silencing of Soybean Seed Storage Proteins Results in a Rebalanced Protein Composition Preserving Seed Protein Content without Major Collateral Changes in the Metabolome and Transcriptome[W][OA]

  2011cited by this paper
• Comprehensive analysis of mitochondria in roots and hypocotyls of soybean under flooding stress using proteomics and metabolomics techniques.

  2011cited by this paper
• Mutation rates in scots pine (Pinus sylvestris L.) from the Chernobyl exclusion zone evaluated with amplified fragment-length polymorphisms (AFLPs) and microsatellite markers.

  2011cited by this paper
• UV radiation reduces epidermal cell expansion in leaves of Arabidopsis thaliana.

  2010cited by this paper
• Plastidial glycolysis in developing Arabidopsis embryos.

  2010cited by this paper
• Proteomics analysis of flax grown in Chernobyl area suggests limited effect of contaminated environment on seed proteome.

  2010cited by this paper
• Meta-analysis of environmental effects on soybean seed composition

  2009cited by this paper
• Proteomic analysis of mature soybean seeds from the Chernobyl area suggests plant adaptation to the contaminated environment.

  2009influential reference
• Characteristic and composition of Jatropha curcas oil seed from Malaysia and its potential as biodiesel feedstock feedstock

  2009cited by this paper
• Proteome analysis of soybean hypocotyl and root under salt stress

  2008cited by this paper
• In-Depth Investigation of the Soybean Seed-Filling Proteome and Comparison with a Parallel Study of Rapeseed1[W][OA]

  2008influential reference
• BjDHNs Confer Heavy-metal Tolerance in Plants

  2008cited by this paper
• Cloning and expression analysis of SKn-type dehydrin gene from bean in response to heavy metals

  2006cited by this paper
• Proteomic Investigation of the Effect of Salicylic Acid on Arabidopsis Seed Germination and Establishment of Early Defense Mechanisms1[W][OA]

  2006cited by this paper
• UPLC/MS(E); a new approach for generating molecular fragment information for biomarker structure elucidation.

  2006cited by this paper
• Proteomic Analysis of Seed Filling in Brassica napus. Developmental Characterization of Metabolic Isozymes Using High-Resolution Two-Dimensional Gel Electrophoresis1[W]

  2006cited by this paper
• A technique for the determination of 90Sr, plutonium and americium isotopes in environmental samples

  2005cited by this paper
• Molecular physiology of legume seed development.

  2005cited by this paper
• A Systematic Proteomic Study of Seed Filling in Soybean. Establishment of High-Resolution Two-Dimensional Reference Maps, Expression Profiles, and an Interactive Proteome Database1[w]

  2005cited by this paper
• Network biology: understanding the cell's functional organization

  2004cited by this paper
• Respiratory metabolism: glycolysis, the TCA cycle and mitochondrial electron transport.

  2004cited by this paper
• Control of storage-product synthesis in seeds.

  2004cited by this paper
• Molecular Aspects of Plant Adaptation to Life in the Chernobyl Zone1[w]

  2004influential reference
• Extremely complex pattern of microsatellite mutation in the germline of wheat exposed to the post-Chernobyl radioactive contamination.

  2003cited by this paper
• Genome hypermethylation in Pinus silvestris of Chernobyl--a mechanism for radiation adaptation?

  2003cited by this paper
• Transgenic tobacco plants expressing a rice cysteine synthase gene are tolerant to toxic levels of cadmium

  2001cited by this paper
• Are storage 2S albumins also defensive proteins

  2001cited by this paper
• The glycine decarboxylase system: a fascinating complex.

  2001cited by this paper
• Germline DNA: Wheat mutation rate after Chernobyl

  2000cited by this paper
• Sucrose transporters in plants: update on function and structure.

  2000cited by this paper
• Overexpression of mitochondrial citrate synthase in Arabidopsis thaliana improved growth on a phosphorus-limited soil.

  2000cited by this paper
• Sugar transporters in higher plants--a diversity of roles and complex regulation.

  2000cited by this paper
• Do legume storage proteins play a role in defending seeds against bruchids?

  2000cited by this paper
• Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana

  1998cited by this paper
• Understanding the Control of Metabolism

  1996cited by this paper
• THE ORGANIZATION AND REGULATION OF PLANT GLYCOLYSIS.

  1996cited by this paper
• Acetyl-coenzyme a can regulate activity of the mitochondrial pyruvate dehydrogenase complex in situ.

  1991cited by this paper
• The Heavy Metal-Binding Peptides of Plants

  1990cited by this paper
• Identification of membrane protein associated with sucrose transport into cells of developing soybean cotyledons.

  1988cited by this paper
• β-ketoacyl-ACP synthase I of Escherichia coli: Nucleotide sequence of thefabB gene and identification of the cerulenin binding residue

  1988cited by this paper
• Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis.

  1986cited by this paper
• Control of Lipid Synthesis during Soybean Seed Development: Enzymic and Immunochemical Assay of Acyl Carrier Protein.

  1984cited by this paper
• Interactions of phosphoenolpyruvate carboxylase and pyruvic kinase in developing soybean seeds

  1981cited by this paper
• Expression of storage-protein genes during soybean seed development

  1981cited by this paper
• Fundamentals of Enzyme Kinetics

  1979cited by this paper
• Pyruvate dehydrogenase complex from higher plant mitochondria and proplastids.

  1977cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• A Theoretical Background to the Use of Measured Concentrations of Intermediates in Study of the Control of Intermediary Metabolism

  1972cited by this paper
• THE ORIGIN OF THE REACTION OF HARDEN AND YOUNG IN CELL-FREE ALCOHOLIC FERMENTATION

  1945cited by this paper
• THE EQUILIBRIA OF ISOMERASE AND ALDOLASE, AND THE PROBLEM OF THE PHOSPHORYLATION OF GLYCERALDEHYDE PHOSPHATE

  1943cited by this paper
• STIMULATORY EFFECTS OF ULTRA-VIOLET RADIATION UPON HIGHER PLANTS.

  1930cited by this paper

• Adaptive Responses in High-Radiation Environments: Insights From Chernobyl Wildlife and Ramsar Residents

  2025cites this paper
• Basidiomycetes to the rescue: Mycoremediation of metal-organics co-contaminated soils.

  2024cites this paper
• Complexity of responses to ionizing radiation in plants, and the impact on interacting biotic factors.

  2024cites this paper
• Biological impact of Chornobyl radiation: a review of recent progress

  2024cites this paper
• Effects of foliar applications of ascobin on seed protein accumulation in soybean (Glycine max (L.) Merrill) JS 1215 grown under cadmium stress

  2023cites this paper
• Changes in DNA Methylation in Arabidopsis thaliana Plants Exposed Over Multiple Generations to Gamma Radiation

  2021influential citation
• What Have We Learned about the Biological Effects of Radiation from the 35 Years of Analysis of the Consequences of the Chernobyl NPP Accident?

  2021cites this paper
• Radioactive contamination in Chernobyl and (epi)genetic stability of plants – A review

  2020cites this paper
• Soybean recovery from stress imposed by multigenerational growth in contaminated Chernobyl environment.

  2020cites this paper
• Fatty acid desaturase 3A gene-specific DNA methylation patterns in flax seeds harvested from a radioactive area near Chernobyl

  2020cites this paper
• Implications of Ionizing Radiation on Pollen Performance in Comparison with Diverse Models of Polar Cell Growth.

  2020cites this paper
• Inter-retrotransposon amplified polymorphism markers revealed long terminal repeat retrotransposon insertion polymorphism in flax cultivated on the experimental fields around Chernobyl

  2020cites this paper
• A review of the impact on the ecosystem after ionizing irradiation wildlife population

  2020cites this paper
• Natural ecotype of Arabidopsis thaliana (L.) Heynh (Chernobyl-07) respond to cadmium stress more intensively than the sensitive ecotypes Oasis and Columbia.

  2019cites this paper
• Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses

  2018cites this paper
• DNA damage, repair monitoring and epigenetic DNA methylation changes in seedlings of Chernobyl soybeans.

  2017cites this paper
• International Plant Proteomics Organization (INPPO) World Congress 2014

  2017influential citation
• The Chernobyl Herbarium: Fragments of an Exploded Consciousness

  2016cites this paper
• Are Organisms Adapting to Ionizing Radiation at Chernobyl?

  2016cites this paper
• Long term effects of Chernobyl contamination on DNA repair function and plant resistance to different biotic and abiotic stress factors

  2016cites this paper
• Systems biology is an efficient tool for investigation of low-dose chronic irradiation influence on plants in the Chernobyl zone

  2016cites this paper
• Do Cupins Have a Function Beyond Being Seed Storage Proteins?

  2016cites this paper
• Long Term Effects of Chernobyl Contamination on DNA Repair Function and Plant Resistance to Different Biotic and Abiotic Stress Factors1

  2016cites this paper
• Lower prevalence but similar fitness in a parasitic fungus at higher radiation levels near Chernobyl

  2016cites this paper
• Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts

  2015cites this paper
• Chernobyl seed project. Advances in the identification of differentially abundant proteins in a radio-contaminated environment

  2015influential citation
• A comparative proteomic analysis of responses to high temperature stress in hypocotyl of Canola (Brassica napus L.).

  2015cites this paper
• Proteomics of field samples in radioactive Chernobyl area.

  2014cites this paper
• Proteomic insights into intra- and intercellular plant-bacteria symbiotic association during root nodule formation

  2013cites this paper
• Radioactive Chernobyl environment has produced high-oil flax seeds that show proteome alterations related to carbon metabolism during seed development.

  2013cites this paper
• Transgenic soybeans and soybean protein analysis: an overview.

  2013cites this paper
• Seeds in Chernobyl: the database on proteome response on radioactive environment

  2012cites this paper