Parallel induction of epithelial surface‐associated chemokine and proteoglycan by cellular hypoxia: implications for neutrophil activation

Neutrophil‐induced damage to the protective epithelium has been implicated in mucosal disorders associated with hypoxia, and such damage may be initiated by epithelial‐derived chemokines. Because chemokines can bind to membrane proteoglycans, we hypothesized that chemokines may associate with epithelial surfaces and activate polymorphonuclear neutrophils (PMN). Epithelial hypoxia (pO2 20 torr) resulted in a time‐dependent induction of interleukin‐8 (IL‐8) mRNA, soluble protein, as well as surface protein. Such surface IL‐8 expression was demonstrated to be dependent on heparinase III expression, and extensions of these experiments indicated that hypoxia induces epithelial perlecan expression in parallel with IL‐8. Finally, co‐incubation of post‐hypoxic epithelia with human PMN induced IL‐8‐dependent expression of the PMN β2‐integrin CD11b/18. These data indicate that chemokines liberated from epithelia may exist in a surface‐bound, bioactive form and that hypoxia may regulate proteoglycan expression.

• Publication year

  2000

• Venue

  Journal of Leukocyte Biology

• Publication date

  2000-08-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1189/jlb.68.2.251PMID 10947070
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Critical Role of cAMP Response Element Binding Protein Expression in Hypoxia-elicited Induction of Epithelial Tumor Necrosis Factor-α*

  1999cited by this paper
• Stromal Cell-derived Factor-1α Associates with Heparan Sulfates through the First β-Strand of the Chemokine*

  1999cited by this paper
• Therapeutic Targets for Hypoxia-Elicited Pathways

  1999cited by this paper
• Structure of a CXC chemokine-receptor fragment in complex with interleukin-8.

  1999cited by this paper
• Knowledge discovery in gene-expression-microarray data: mining the information output of the genome.

  1999cited by this paper
• Identification of a glycosaminoglycan binding surface on human interleukin-8.

  1998cited by this paper
• Proteoglycans: master regulators of molecular encounter?

  1998cited by this paper
• Activated endothelial cells elicit paracrine induction of epithelial chloride secretion. 6-Keto-PGF1alpha is an epithelial secretagogue.

  1998cited by this paper
• Autocrine regulation of epithelial permeability by hypoxia: role for polarized release of tumor necrosis factor alpha.

  1998cited by this paper
• The Central Role of Chemokines (Chemotactic Cytokines) in the Immunopathogenesis of Ulcerative Colitis and Crohn's Disease

  1998cited by this paper
• Hypoxia inhibits cyclic nucleotide-stimulated epithelial ion transport: role for nucleotide cyclases as oxygen sensors.

  1998cited by this paper
• Hypoxia Induces Phosphorylation of the Cyclic AMP Response Element-binding Protein by a Novel Signaling Mechanism*

  1998influential reference
• Chemokines and leukocyte traffic

  1998cited by this paper
• Epithelial cells as sensors for microbial infection.

  1997cited by this paper
• Molecular events in neutrophil transepithelial migration

  1997cited by this paper
• Epithelial exposure to hypoxia modulates neutrophil transepithelial migration

  1996cited by this paper
• Expression monitoring by hybridization to high-density oligonucleotide arrays

  1996cited by this paper
• Cyclic AMP regulates basement membrane heparan sulfate proteoglycan, perlecan, metabolism in rat glomerular epithelial cells

  1996cited by this paper
• CD47 mediates post-adhesive events required for neutrophil migration across polarized intestinal epithelia

  1996cited by this paper
• Hypoxia enhances stimulus-dependent induction of E-selectin on aortic endothelial cells.

  1996cited by this paper
• Receptors Involved in Carbohydrate Binding Modulate Intestinal Epithelial-Neutrophil Interactions

  1995cited by this paper
• Heparin is an adhesive ligand for the leukocyte integrin Mac-1 (CD11b/CD1)

  1995cited by this paper
• The IP-10 chemokine binds to a specific cell surface heparan sulfate site shared with platelet factor 4 and inhibits endothelial cell proliferation

  1995cited by this paper
• Inherited disorders of coagulation appear to protect against inflammatory bowel disease.

  1995cited by this paper
• Endothelial cell tolerance to hypoxia. Potential role of purine nucleotide phosphates.

  1995cited by this paper
• Intestinal epithelia (T84) possess basolateral ligands for CD11b/CD18-mediated neutrophil adherence.

  1995cited by this paper
• Surface attachment of Salmonella typhimurium to intestinal epithelia imprints the subepithelial matrix with gradients chemotactic for neutrophils

  1995influential reference
• Hypoxic induction of interleukin-8 gene expression in human endothelial cells.

  1994cited by this paper
• Interleukin-8 and related chemotactic cytokines--CXC and CC chemokines.

  1994cited by this paper
• Interleukin-8 and related chemotactic cytokines. The Giles Filley Lecture.

  1994cited by this paper
• Differential binding of chemokines to glycosaminoglycan subpopulations.

  1994influential reference
• Neutrophil migration across cultured intestinal epithelial monolayers is modulated by epithelial exposure to IFN-gamma in a highly polarized fashion

  1993cited by this paper
• Restoration of the cAMP second messenger pathway enhances cardiac preservation for transplantation in a heterotopic rat model.

  1993cited by this paper
• Structural characterization of the complete human perlecan gene and its promoter.

  1993cited by this paper
• A simple approach to measurement of electrical parameters of cultured epithelial monolayers: Use in assessing neutrophil-epithelial interactions

  1992cited by this paper
• Granulocyte accumulation in postischemic intestine: role of leukocyte adhesion glycoprotein CD11/CD18.

  1992cited by this paper
• Biology and biochemistry of the chemokines: a family of chemotactic and inflammatory cytokines.

  1992cited by this paper
• Pathophysiology of ischaemia reperfusion injury: Central role of the neutrophil

  1991cited by this paper
• In vitro model of intestinal crypt abscess. A novel neutrophil-derived secretagogue activity.

  1991cited by this paper
• Neutrophil migration across a cultured intestinal epithelium. Dependence on a CD11b/CD18-mediated event and enhanced efficiency in physiological direction.

  1991cited by this paper
• Established intestinal cell lines as model systems for electrolyte transport studies.

  1990cited by this paper
• Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8.

  1989cited by this paper
• Pathogenesis of Crohn's disease: multifocal gastrointestinal infarction.

  1989cited by this paper
• Vectorial targeting of apical and basolateral plasma membrane proteins in a human adenocarcinoma epithelial cell line.

  1989cited by this paper
• On the pathophysiology of intestinal ischemic injury. Clinical review.

  1987cited by this paper
• Effects of polymorphonuclear leukocyte transmigration on the barrier function of cultured intestinal epithelial monolayers.

  1987cited by this paper
• Contributions of ischemia and reperfusion to mucosal lesion formation.

  1986cited by this paper
• Electrolyte transport across colonic mucosa from patients with inflammatory bowel disease.

  1980cited by this paper
• Stimulation of human neutrophils by soluble and insoluble immunoglobulin aggregates. Secretion of granule constituents and increased oxidation of glucose.

  1975cited by this paper
• recurrence: microvascular ischaemia and anastornotic healing in an animal model

  year unknowncited by this paper

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  2017cites this paper
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  2014cites this paper
• Microparticles derived from stimulation of human umbilical endothelium

  2013cites this paper
• Die Auswirkung von Hypoxie auf die intestinale Tight Junction: Rolle des Transkriptionsfaktors Hypoxia Inducible Factor 1 (HIF-1)

  2011cites this paper
• Hypoxia inducible factor‐1 (HIF‐l)‐mediated repression of cystic fibrosis transmembrane conductance regulator (CFTR) in the intestinal epithelium

  2009cites this paper
• Butyrate Regulates the Expression of Pathogen-Triggered IL-8 in Intestinal Epithelia

  2007cites this paper
• Mechanisms of Cell Growth Regulation by Heparin and Heparan Sulfate

  2005cites this paper
• Endothelial activation and increased heparan sulfate expression in cystic fibrosis.

  2005cites this paper
• Implications of hypoxia on mucosal barrier function.

  2003cites this paper
• Syndecans in inflammation

  2003cites this paper
• Epithelial Cell-Neutrophil Interactions in the Alimentary Tract: A Complex Dialog in Mucosal Surveillance and Inflammation

  2002cites this paper
• Fractalkine-mediated signals regulate cell-survival and immune-modulatory responses in intestinal epithelial cells.

  2002cites this paper
• During Hypoxia Expression α Proliferator-Activated Receptor Inhibition of Peroxisome Hypoxia-Inducible Factor 1-Mediated

  2001cites this paper
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  2001cites this paper
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  2001cites this paper