Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development

Developmentally regulated delayed rectifier potassium currents determine the waveform of the action potential in all Xenopus embryonic primary spinal neurons. To examine this developmental program at the molecular level, we have isolated Xenopus Kv2 potassium channel genes Kv2.1 and Kv2.2. Both genes induce functional heterologous expression of delayed rectifier potassium currents. Transcripts from both Kv2 genes are present in developing embryos; however, only Kv2.2 mRNA is detectable in embryonic spinal neurons. Notably, Kv2.2 transcripts localize to ventral spinal neurons, whereas previously described Kv1.1 transcripts are found in dorsal spinal neurons. Thus, spinal neuron subtypes express distinct potassium channel genes, yet they temporally coordinate functional expression of delayed rectifier potassium currents.

• Publication year

  1996

• Venue

  Journal of Neuroscience

• Publication date

  1996-02-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1523/JNEUROSCI.16-04-01412.1996PMID 8778292PMCID PMC6578550
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Homogeneous development of electrical excitability via heterogeneous ion channel expression

  1996cited by this paper
• The major delayed rectifier in both Drosophila neurons and muscle is encoded by Shab

  1995cited by this paper
• Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein.

  1995influential reference
• Overexpression of a potassium channel gene perturbs neural differentiation

  1994influential reference
• A Shab potassium channel contributes to action potential broadening in peptidergic neurons.

  1994cited by this paper
• Properties of Kv2.1 K+ channels expressed in transfected mammalian cells.

  1994cited by this paper
• Inactivation properties of voltage-gated K+ channels altered by presence of β-subunit

  1994cited by this paper
• Gating and conductance properties of a human delayed rectifier K+ channel expressed in frog oocytes.

  1994influential reference
• Regulation of Shaker K+ channel inactivation gating by the cAMP-dependent protein kinase.

  1994cited by this paper
• Contrasting immunohistochemical localizations in rat brain of two novel K+ channels of the Shab subfamily

  1993cited by this paper
• Cloning and characterization of a human delayed rectifier potassium channel gene.

  1993influential reference
• Primary sensory neurons express a Shaker-like potassium channel gene

  1993cited by this paper
• Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast

  1993cited by this paper
• A novel K+ channel with unique localizations in mammalian brain: molecular cloning and characterization.

  1992influential reference
• Distinct spatial and temporal expression patterns of K+ channel mRNAs from different subfamilies

  1992cited by this paper
• Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.

  1992influential reference
• Developmental sequence of expression of voltage-dependent currents in embryonic Xenopus laevis myocytes.

  1992cited by this paper
• Developmental regulation of potassium channels and the impact on neuronal differentiation.

  1992cited by this paper
• Immunological identification and characterization of a delayed rectifier K+ channel polypeptide in rat brain.

  1991cited by this paper
• In situ hybridization: an improved whole-mount method for Xenopus embryos.

  1991cited by this paper
• Differentiation of delayed rectifier potassium current in embryonic amphibian myocytes.

  1991cited by this paper
• A mouse brain homolog of the Drosophila Shab K+ channel with conserved delayed-rectifier properties

  1991cited by this paper
• Simplified gene nomenclature

  1991cited by this paper
• Role of calcium and protein kinase C in development of the delayed rectifier potassium current in Xenopus spinal neurons.

  1991cited by this paper
• Phosphorylation affects voltage gating of the delayed rectifier K+ channel by electrostatic interactions.

  1990cited by this paper
• Protein kinase recognition sequence motifs.

  1990cited by this paper
• A delayed rectifier potassium current in Xenopus oocytes.

  1990cited by this paper
• A potassium channel gene is expressed at neural induction.

  1990influential reference
• The mRNA encoding elongation factor 1-alpha (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus.

  1989cited by this paper
• A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning

  1989influential reference
• A critical period of transcription required for differentiation of the action potential of spinal neurons.

  1989influential reference
• A family of putative potassium channel genes in Drosophila.

  1989cited by this paper
• The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus

  1989cited by this paper
• Modulation of ion channels in neurons and other cells.

  1988cited by this paper
• Development of voltage-dependent calcium, sodium, and potassium currents in Xenopus spinal neurons

  1988cited by this paper
• Expression of Xenopus N-CAM RNA in ectoderm is an early response to neural induction.

  1987cited by this paper
• Assembly of asparagine-linked oligosaccharides.

  1985cited by this paper
• Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

  1985cited by this paper
• A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.

  1982cited by this paper
• DNA sequencing with chain-terminating inhibitors.

  1977influential reference
• Normal Table of Xenopus laevis

  1957cited by this paper

• Electrical Properties of Adult Mammalian Motoneurons.

  2022cites this paper
• Location, location, location: the organization and roles of potassium channels in mammalian motoneurons

  2021cites this paper
• A molecular rheostat: Kv2.1 currents maintain or suppress repetitive firing in motoneurons

  2019cites this paper
• HCN2 Rescues brain defects by enforcing endogenous voltage pre-patterns

  2018cites this paper
• Xenopus laevis as a Model Organism for the Study of Spinal Cord Formation, Development, Function and Regeneration

  2017cites this paper
• Title Xenopus laevis as a Model Organism for the Study of Spinal Cord Formation , Development , Function and Regeneration Permalink

  2017cites this paper
• Dissecting the Molecular Relationship Among Various Cardiogenic Progenitor Cells

  2013cites this paper
• Xaml1/Runx1 is required for the specification of Rohon-Beard sensory neurons in Xenopus.

  2012cites this paper
• Subunit-Dependent Axonal Trafficking of Distinct α Heteromeric Potassium Channel Complexes

  2011influential citation
• Neurite Outgrowth and In Vivo Sensory Innervation Mediated by a CaV2.2–Laminin β2 Stop Signal

  2008cites this paper
• Localization of Kv2.2 protein in Xenopus laevis embryos and tadpoles

  2008influential citation
• NeuriteOutgrowthandInVivoSensoryInnervation MediatedbyaCa V 2.2-Laminin2StopSignal

  2008cites this paper
• Dorsal–Ventral Gradient for Neuronal Plasticity in the Embryonic Spinal Cord

  2008influential citation
• Kv1 potassium channel complexes in vivo require Kvbeta2 subunits in dorsal spinal neurons.

  2008cites this paper
• Voltage-Gated K+ Channel Dysfunction in Myocytes from a Dog Model of Subarachnoid Hemorrhage

  2008cites this paper
• Evolution of the Action Potential

  2007cites this paper
• Developmental regulation of the expression of sodium currents in Xenopus primary neurons.

  2006cites this paper
• Voltage‐gated potassium channels regulate the response of retinal growth cones to axon extension and guidance cues

  2005cites this paper
• Running Head: In situ regulation of Kv2 channel function

  2004cites this paper
• Carboxyl tail region of the Kv2.2 subunit mediates novel developmental regulation of channel density.

  2004cites this paper
• Properties of Xenopus Kv1.10 channels expressed in HEK293 cells.

  2004cites this paper
• Selective regulation of xSlo splice variants during Xenopus embryogenesis.

  2003cites this paper
• Early embryonic expression of ion channels and pumps in chick and Xenopus development

  2002cites this paper
• Expression of voltage‐dependent potassium channels in the developing visual system of Xenopus laevis

  2002cites this paper
• In vivo analysis of Kvβ2 function in Xenopus embryonic myocytes

  2002cites this paper
• A Shaker homologue encodes an A-type current in Xenopus laevis.

  2002cites this paper
• In vivo analysis of Kvbeta2 function in Xenopus embryonic myocytes.

  2002cites this paper
• Repressor Element-1 Silencing Transcription/Neuron-Restrictive Silencer Factor Is Required for Neural Sodium Channel Expression during Development of Xenopus

  2002cites this paper
• Physiological effects of sustained blockade of excitatory synaptic transmission on spontaneously active developing neuronal networks--an inquiry into the reciprocal linkage between intrinsic biorhythms and neuroplasticity in early ontogeny.

  2002cites this paper
• Perinatal changes of I(h) in phrenic motoneurons.

  2001cites this paper
• Molecular cloning and functional characterization of a novel delayed rectifier potassium channel from channel catfish (Ictalurus punctatus): expression in taste buds

  2001cites this paper
• Kv2 Channels Form Delayed-Rectifier Potassium Channels In Situ

  2001influential citation
• Perinatal changes of Ih in phrenic motoneurons

  2001cites this paper
• Cloning and expression of three K+ channel cDNAs from Xenopus muscle.

  2001cites this paper
• Sustained upregulation in embryonic spinal neurons of a Kv3.1 potassium channel gene encoding a delayed rectifier current.

  2000cites this paper
• Antisense Suppression of Potassium Channel Expression Demonstrates Its Role in Maturation of the Action Potential

  2000influential citation
• Regulation of Calcineurin by Growth Cone Calcium Waves Controls Neurite Extension

  2000cites this paper
• Expression of Shal potassium channel subunits in the adult and developing cochlear nucleus of the mouse.

  2000cites this paper
• Specific frequencies of spontaneous Ca2+ transients upregulate GAD 67 transcripts in embryonic spinal neurons.

  2000cites this paper
• A Role for Voltage-Gated Potassium Channels in the Outgrowth of Retinal Axons in the Developing Visual System

  2000cites this paper
• Differentiation of electrical excitability in motoneurons.

  2000cites this paper
• Development of potassium conductances in perinatal rat phrenic motoneurons.

  2000cites this paper
• Subfamily-Specific Posttranscriptional Mechanism Underlies K+ Channel Expression in a Developing Neuronal Blastomere

  1999cites this paper
• Xenopus Embryonic Spinal Neurons Express Potassium Channel Kvβ Subunits

  1999influential citation
• Potassium Currents in Developing Neurons

  1999cites this paper
• VLG K Kv1-Shak: Vertebrate K+ channels related to Drosophila Shaker (Kvα subunits encoded by gene subfamily Kv1)

  1999cites this paper
• Detection of transcripts for delayed rectifier potassium channels in the Xenopus laevis inner ear.

  1998cites this paper
• The Pharmacology and Roles of two K+ Channels in Motor Pattern Generation in the Xenopus Embryo

  1998cites this paper
• Development of electrical excitability in embryonic neurons: mechanisms and roles.

  1998cites this paper
• Expression of Kv1.1, a Shaker-Like Potassium Channel, Is Temporally Regulated in Embryonic Neurons and Glia

  1998cites this paper
• Expression of Kv 1 . 1 , a Shaker-Like Potassium Channel , Is Temporally Regulated in Embryonic Neurons and Glia

  1998cites this paper
• Heteromultimeric Potassium Channels Formed by Members of the Kv2 Subfamily

  1998influential citation
• RNA editing generates a diverse array of transcripts encoding squid Kv2 K+ channels with altered functional properties.

  1997cites this paper
• The Drosophila islet gene governs axon pathfinding and neurotransmitter identity.

  1997cites this paper
• Temporal Regulation of Shaker- andShab-Like Potassium Channel Gene Expression in Single Embryonic Spinal Neurons during K+ Current Development

  1996influential citation
• Probing molecular identity of native single potassium channels by overexpression of dominant negative subunits.

  1996cites this paper