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Reduced mineral oil ratio improves blastocyst yield in well-ofthe-well ( WOW ) and polyester mesh ( PM ) single-embryo cultures-short communication

B. E. Pereda-Espinoza,M. Burrola-Barraza,F. Rodríguez-Almeida,J. Antillón-Ruiz,A. Anchondo-Garay

Published 2016 in Unknown venue

ABSTRACT

It is common to apply a mineral oil (MO) overlay to in vitro embryo cultures, even though the use of MO may be harmful to embryo development. To examine whether the volume of MO used in overlays for well-of-the-well (WOW) and polyester mesh (PM) single-embryo culture systems can be reduced, two sets of experiments were performed and the resulting blastocyst formation rates were compared with a conventional group (CG) embryo culture system. In Experiment 1, groups of 20 embryos in 100 μL microdrops of CDM-2 medium were plated in Petri dishes and then were covered with 3.5 mL of MO (resulting in a 1:35 ratio of medium to MO). Groups of 20 embryos were also placed in four-well plates in 400 μL CDM-2 medium per well, and the wells were covered with 400 μL MO (resulting in a 1:1 ratio of medium to MO). In Experiment 2, groups of 20 embryos were plated in four-well plates with 400 μL CDM-2 medium per well, and 1 mL of purifi ed water was added to the center hole of the plate. Two sets of these plates were set up in parallel, and only one of the plates received an additional 400 μL of MO per well (resulting in a 1:1 ratio of medium to MO). A greater percentage of embryos reached the blastocyst stage when they were cultured in a 1:1 ratio of medium to MO, compared with embryos that were cultured in a 1:35 ratio of medium to MO (55 % vs. 41 %, respectively; P<0.01). In addition, a greater percentage of embryos reached the blastocyst stage in the WOW and PM systems that included a 1:1 ratio of medium to MO overlay than the embryos that were cultured in the presence of water in the central hole of a four-well plate without MO (48 % vs. 39 %, respectively; P<0.01). Therefore, a 1:1 ratio of medium to MO was found to improve blastocyst rates in both WOW and PM embryo culture systems.

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