An efficient gene deletion procedure for the mushroom-forming basidiomycete Schizophyllum commune

Gene deletion in Schizophyllum commune is hampered by a low incidence of homologous integration. As a consequence, extensive screening is required to identify a transformant with the desired genotype. To alleviate this and to facilitate the assembly of deletion plasmids, vector pDelcas was constructed. This construct has a set of restriction sites, which allows for directional cloning of the flanking sequences at both sides of a nourseothricin resistance cassette. Moreover, it contains a phleomycin resistance cassette elsewhere in the plasmid, which is used to screen for transformants with an ectopic integration of the pDelcas derivative. The use of pDelcas derivatives in combination with an improved PCR screening protocol permitted the efficient identification of S. commune deletion strains. This procedure may also function in other basidiomycetes.

• Publication year

  2010

• Venue

  World Journal of Microbiology & Biotechnology

• Publication date

  2010-02-27

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1007/s11274-010-0356-0PMID 20930926PMCID 2940052
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Phleomycin Increases Transformation Efficiency and Promotes Single Integrations in Schizophyllum commune

  2008influential reference
• Ras GTPase-Activating Protein Gap1 of the Homobasidiomycete Schizophyllum commune Regulates Hyphal Growth Orientation and Sexual Development

  2006cited by this paper
• The Aα mating-type locus ofSchizophyflum commune: Structure and function of geneX

  2005cited by this paper
• The SC 15 protein of Schizophyllum commune mediates formation of aerial hyphae and attachment in the absence of the SC 3 hydrophobin

  2004cited by this paper
• The SC15 protein of Schizophyllum commune mediates formation of aerial hyphae and attachment in the absence of the SC3 hydrophobin

  2004cited by this paper
• Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining.

  2004cited by this paper
• Highly Efficient Production of Laccase by the Basidiomycete Pycnoporus cinnabarinus

  2004cited by this paper
• SC3 and SC4 hydrophobins have distinct roles in formation of aerial structures in dikaryons of Schizophyllum commune

  2000cited by this paper
• Mated: a putative peptide transporter of Schizophyllum commune expressed in dikaryons

  1999cited by this paper
• Multiple sex pheromones and receptors of a mushroom-producing fungus elicit mating in yeast.

  1999cited by this paper
• Identification and characterization of brt1, a gene down-regulated during B-regulated development in Schizophyllum commune

  1999cited by this paper
• Regulation of dikaryon-expressed genes by FRT1 in the basidiomycete Schizophyllum commune.

  1999cited by this paper
• Deletion of the Schizophyllum commune A alpha locus: the roles of A alpha Y and Z mating-type genes.

  1996cited by this paper
• Deletion of the Schizophyllum Commune Aα Locus: The Roles of Aα Y and Z Mating-Type Genes

  1996cited by this paper
• The A alpha mating-type locus of Schizophyllum commune: structure and function of gene X.

  1996cited by this paper
• Targeted mutation of the SC3 hydrophobin gene of Schizophyllum commune affects formation of aerial hyphae

  1996cited by this paper
• Research letterTargeted mutation of the SC3 hydrophobin gene of Schizophyllum commune affects formation of aerial hyphae

  1996cited by this paper
• Highly-efficient transformation of the homobasidiomycete Schizophyllum commune to phleomycin resistance

  1994cited by this paper
• THE FLR1 GENE, A USEFUL SYSTEM FOR RAPID SCREENING OF TRYPTOPHAN AUXOTROPHS IN SCHIZOPHYLLUM COMMUNE

  1993cited by this paper
• Characterization of the genome of the basidiomycete Schizophyllum commune.

  1979cited by this paper

• Comparative transcriptome analyses and CRISPR/Cas9-mediated functional study of Tfsdh1 reveal insights into the interaction between Tremella fuciformis and Annulohypoxylon stygium

  2026cites this paper
• Functions of the cell wall polysaccharide schizophyllan during vegetative growth of Schizophyllum commune

  2025cites this paper
• Strongest untreated mycelium materials produced by Schizophyllum commune dikaryons

  2025influential citation
• Targeted insertion of heterogenous DNA using Cas9-gRNA ribonucleoprotein-mediated gene editing in Ganoderma lucidum

  2025cites this paper
• CRISPR/Cas9-mediated genome editing in Ganoderma lucidum: recent advances and biotechnological opportunities

  2025influential citation
• Transposition of a non-autonomous element into the Gβ gene of Schizophyllum commune causes the streak mutation.

  2025cites this paper
• CRISPR/Cas9-mediated gene editing in filamentous fungi, focusing on mushrooms and plant-pathogenic fungi

  2025cites this paper
• Transcriptomics reveals the regulation of the immune system of the mushroom-forming fungus Schizophyllum commune during interaction with four competitors.

  2024cites this paper
• Effect of a Mating Type Gene Editing in Lentinula edodes Using RNP/Nanoparticle Complex

  2024cites this paper
• Biochemical characterization of an organic solvent- and salt-tolerant xylanase and its application of arabinoxylan-oligosaccharides production from corn fiber gum.

  2024cites this paper
• A journey with psychedelic mushrooms: From historical relevance to biology, cultivation, medicinal uses, biotechnology, and beyond.

  2023cites this paper
• Optimization of protein hydrolysate preparation from split gill mushroom Schizophyllum commune

  2023cites this paper
• The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments

  2023cites this paper
• Genome, genetic evolution, and environmental adaptation mechanisms of Schizophyllum commune in deep subseafloor coal-bearing sediments

  2022influential citation
• CRISPR-Cas9 assisted in-situ complementation of functional genes in the basidiomycete Ganoderma lucidum

  2022cites this paper
• Preassembled Cas9 Ribonucleoprotein-Mediated Gene Deletion Identifies the Carbon Catabolite Repressor and Its Target Genes in Coprinopsis cinerea

  2022cites this paper
• Visualising long distance sugar transport in fungi using infrared fluorescence scanning imaging.

  2022cites this paper
• A screening method for decoloration of xenobiotic dyes by fungi.

  2021cites this paper
• Targeted gene knock-in reduces variation between transformants in the mushroom-forming fungus Schizophyllum commune

  2021cites this paper
• Microbial hosts for metabolic engineering of lignin bioconversion to renewable chemicals

  2021cites this paper
• Comparison of cell wall polysaccharides in Schizophyllum commune after changing phenotype by mutation.

  2021cites this paper
• H3K4me2 ChIP-Seq reveals the epigenetic landscape during mushroom formation and novel developmental regulators of Schizophyllum commune

  2021cites this paper
• Fungal evolution: cellular, genomic and metabolic complexity

  2020cites this paper
• CRISPR-Cas9 system for fungi genome engineering toward industrial applications

  2020cites this paper
• Wood-Rotting Fungi for Biofuel Production

  2020cites this paper
• Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research

  2020cites this paper
• Interruption of an MSH4 homolog blocks meiosis in metaphase I and eliminates spore formation in Pleurotus ostreatus

  2020cites this paper
• Fungi in Fuel Biotechnology

  2020cites this paper
• Electron beam irradiation on Fuzhuan brick-tea: Effects on sensory quality and chemical compositions

  2020cites this paper
• CRISPR-Cas9 assisted functional gene editing in the mushroom Ganoderma lucidum

  2019cites this paper
• High-throughput targeted gene deletion in the model mushroom Schizophyllum commune using pre-assembled Cas9 ribonucleoproteins

  2019cites this paper
• The regulator of G‐protein signalling Thn1 links pheromone response to volatile production in Schizophyllum commune

  2018cites this paper
• Schizophyllum commune: An unexploited source for lignocellulose degrading enzymes

  2018cites this paper
• Bioinformatic Analysis of Genomic and Transcriptomic Variation in Fungi

  2018cites this paper
• Nucleus-specific expression in the multinuclear mushroom-forming fungus Agaricus bisporus reveals different nuclear regulatory programs

  2017cites this paper
• Variants in RNA-Seq data show a continued mutation rate during strain preservation of Schizophyllum commune

  2017cites this paper
• Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth

  2017cites this paper
• CRISPR-Cas9 assisted gene disruption in the higher fungus Ganoderma species

  2017cites this paper
• Production of Useful Terpenoids by Higher-Fungus Cell Factory and Synthetic Biology Approaches.

  2016cites this paper
• The good, the bad and the tasty: The many roles of mushrooms

  2016cites this paper
• Schizophyllum commune has an extensive and functional alternative splicing repertoire

  2016cites this paper
• Genome engineering of woody plants: past, present and future

  2016cites this paper
• Cell Factories of Higher Fungi for Useful Metabolite Production.

  2015cites this paper
• Mating-type genes and hyphal fusions in filamentous basidiomycetes

  2015cites this paper
• Production of (+)-valencene in the mushroom-forming fungus S. commune

  2014cites this paper
• VeA of Aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture

  2014cites this paper
• Inhibition of secretion by asexual reproduction in Aspergillus niger

  2014cites this paper
• The blue light receptor complex WC-1/2 of Schizophyllum commune is involved in mushroom formation and protection against phototoxicity.

  2013influential citation
• N-GLYCOSYLATION AND GLYCOENGINEERING IN THE MUSHROOM FORMING FUNGUS SCHIZOPHYLLUM COMMUNE

  2013cites this paper
• Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the Δalg3 knockout mutant.

  2013cites this paper
• Xenomic networks variability and adaptation traits in wood decaying fungi

  2013cites this paper
• Diversité fonctionelle des Glutation Transférases fongiques : caractérisation des classes Ure2p et GTT2 de Phanerochaete chrysosporium

  2013cites this paper
• Caractérisation structurale et enzymatique, cibles potentielles et rôles physiologiques de glutathion transférases à cystéine catalytique de Phanerochaete chrysosporium

  2012cites this paper
• The function of Schizophyllum commune agglutinin 1 in the defense against fungivorous nematodes Ditylenchus myceliophagus

  2012cites this paper
• Sexual selection in fungi

  2012cites this paper
• Transcription factor genes of Schizophyllum commune involved in regulation of mushroom formation

  2011influential citation
• Formation of Mushrooms and Lignocellulose Degradation Encoded in the Genomes Sequence of Schizophyllum Commune

  2011cites this paper
• Regulation of mushroom formation in Schizophyllum commune

  2010cites this paper
• Inactivation of ku80 in the mushroom-forming fungus Schizophyllum commune increases the relative incidence of homologous recombination.

  2010influential citation
• Genome sequence of the model mushroom Schizophyllum commune

  2010cites this paper